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Annotation of a 95-kb Populus deltoides genomic sequence reveals a disease resistance gene cluster and novel class I and class II transposable elements

Magali Lescot (UGent) , Stephane Rombauts (UGent) , Jiong Zhang (UGent) , Sébastien Aubourg (UGent) , Catherine Mathé (UGent) , S Jansson, Pierre Rouzé (UGent) and Wout Boerjan (UGent)
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Abstract
Poplar has become a model system for functional genomics in woody plants. Here, we report the sequencing and annotation of the first large contiguous stretch of genomic sequence (95 kb) of poplar, corresponding to a bacterial artificial chromosome clone mapped 0.6 centiMorgan from the Melampsora larici-populina resistance locus. The annotation revealed 15 putative genetic objects, of which five were classified as hypothetical genes that were similar only with expressed sequence tags from poplar. Ten putative objects showed similarity with known genes, of which one was similar to a kinase. Three other objects corresponded to the toll/interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat class of plant disease resistance genes, of which two were predicted to encode an amino terminal nuclear localization signal. Four objects were homologous to the Ty1/copia family of class I transposable elements, one of which was designated Retropop and interrupted one of the disease resistance genes. Two other objects constituted a novel Spm-like class II transposable element, which we designated Magali.
Keywords
EVOLUTION, RETROTRANSPOSONS, DNA, LEAF RUST, MAJOR GENE, HYBRID POPLAR, CONFERRING RESISTANCE, ARABIDOPSIS-THALIANA, MELAMPSORA-LARICI-POPULINA, DOWNY MILDEW RESISTANCE

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MLA
Lescot, Magali, Stephane Rombauts, Jiong Zhang, et al. “Annotation of a 95-kb Populus Deltoides Genomic Sequence Reveals a Disease Resistance Gene Cluster and Novel Class I and Class II Transposable Elements.” THEORETICAL AND APPLIED GENETICS 109.1 (2004): 10–22. Print.
APA
Lescot, M., Rombauts, S., Zhang, J., Aubourg, S., Mathé, C., Jansson, S., Rouzé, P., et al. (2004). Annotation of a 95-kb Populus deltoides genomic sequence reveals a disease resistance gene cluster and novel class I and class II transposable elements. THEORETICAL AND APPLIED GENETICS, 109(1), 10–22.
Chicago author-date
Lescot, Magali, Stephane Rombauts, Jiong Zhang, Sébastien Aubourg, Catherine Mathé, S Jansson, Pierre Rouzé, and Wout Boerjan. 2004. “Annotation of a 95-kb Populus Deltoides Genomic Sequence Reveals a Disease Resistance Gene Cluster and Novel Class I and Class II Transposable Elements.” Theoretical and Applied Genetics 109 (1): 10–22.
Chicago author-date (all authors)
Lescot, Magali, Stephane Rombauts, Jiong Zhang, Sébastien Aubourg, Catherine Mathé, S Jansson, Pierre Rouzé, and Wout Boerjan. 2004. “Annotation of a 95-kb Populus Deltoides Genomic Sequence Reveals a Disease Resistance Gene Cluster and Novel Class I and Class II Transposable Elements.” Theoretical and Applied Genetics 109 (1): 10–22.
Vancouver
1.
Lescot M, Rombauts S, Zhang J, Aubourg S, Mathé C, Jansson S, et al. Annotation of a 95-kb Populus deltoides genomic sequence reveals a disease resistance gene cluster and novel class I and class II transposable elements. THEORETICAL AND APPLIED GENETICS. 2004;109(1):10–22.
IEEE
[1]
M. Lescot et al., “Annotation of a 95-kb Populus deltoides genomic sequence reveals a disease resistance gene cluster and novel class I and class II transposable elements,” THEORETICAL AND APPLIED GENETICS, vol. 109, no. 1, pp. 10–22, 2004.
@article{298971,
  abstract     = {Poplar has become a model system for functional genomics in woody plants. Here, we report the sequencing and annotation of the first large contiguous stretch of genomic sequence (95 kb) of poplar, corresponding to a bacterial artificial chromosome clone mapped 0.6 centiMorgan from the Melampsora larici-populina resistance locus. The annotation revealed 15 putative genetic objects, of which five were classified as hypothetical genes that were similar only with expressed sequence tags from poplar. Ten putative objects showed similarity with known genes, of which one was similar to a kinase. Three other objects corresponded to the toll/interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat class of plant disease resistance genes, of which two were predicted to encode an amino terminal nuclear localization signal. Four objects were homologous to the Ty1/copia family of class I transposable elements, one of which was designated Retropop and interrupted one of the disease resistance genes. Two other objects constituted a novel Spm-like class II transposable element, which we designated Magali.},
  author       = {Lescot, Magali and Rombauts, Stephane and Zhang, Jiong and Aubourg, Sébastien and Mathé, Catherine and Jansson, S and Rouzé, Pierre and Boerjan, Wout},
  issn         = {0040-5752},
  journal      = {THEORETICAL AND APPLIED GENETICS},
  keywords     = {EVOLUTION,RETROTRANSPOSONS,DNA,LEAF RUST,MAJOR GENE,HYBRID POPLAR,CONFERRING RESISTANCE,ARABIDOPSIS-THALIANA,MELAMPSORA-LARICI-POPULINA,DOWNY MILDEW RESISTANCE},
  language     = {eng},
  number       = {1},
  pages        = {10--22},
  title        = {Annotation of a 95-kb Populus deltoides genomic sequence reveals a disease resistance gene cluster and novel class I and class II transposable elements},
  url          = {http://dx.doi.org/10.1007/s00122-004-1621-0},
  volume       = {109},
  year         = {2004},
}

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