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Differential expression of fructan 1-exohydrolase genes involved in inulin biodegradation in chicory (Cichorium intybus) cultivars

Asaf Maroufi, Erik Van Bockstaele UGent and Marc De Loose UGent (2012) AUSTRALIAN JOURNAL OF CROP SCIENCE. 6(9). p.1362-1368
abstract
Fructan 1-exohydrolase (1-FEH; FEH) enzymes are involved in inulin degradation in the roots of chicory. rehgiH FEH expression in cold temperatures can decrease the quality and the quantity of the inulin. This is the case at the end of the growing season and during cold storage. Little is known at molecular level whether the expression levels of fructan 1-exohydrolase genes vary among chicory cultivars, especially during cold storage of roots. Real-time RT-PCR is the most sensitive method for the detection of low abundance mRNAs. Quantitative real time PCR (RT-qPCR) analysis of the FEH mRNAs (including FEHI and FEHII) in cold stored roots of three chicory cultivars was studied. RT-qPCR results showed variable levels of FEHII and FEHI expression in different hgitonehis of the chicory cultivars studied. Moreover, longer cold storage of roots significantly induced expression levels of FEHII in some chicory phenotypes. These results could be used in breeding programmes to increase inulin content in root chicory by selecting genotypes based on their FEH expression profiles.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
cold storage, quantitative real time PCR, breeding, root chicory, inulin degradation
journal title
AUSTRALIAN JOURNAL OF CROP SCIENCE
Aust. J. Crop Sci.
volume
6
issue
9
pages
1362 - 1368
ISSN
1835-2707
language
English
UGent publication?
yes
classification
A2
copyright statement
I have retained and own the full copyright for this publication
id
2988698
handle
http://hdl.handle.net/1854/LU-2988698
alternative location
http://www.cropj.com/maroufi_6_9_2012_1362_1368.pdf
date created
2012-09-18 07:47:06
date last changed
2012-09-19 09:28:08
@article{2988698,
  abstract     = {Fructan 1-exohydrolase (1-FEH; FEH) enzymes are involved in inulin degradation in the roots of chicory. rehgiH FEH expression in cold temperatures can decrease the quality and the quantity of the inulin. This is the case at the end of the growing season and during cold storage. Little is known at molecular level whether the expression levels of fructan 1-exohydrolase genes vary among chicory cultivars, especially during cold storage of roots. Real-time RT-PCR is the most sensitive method for the detection of low abundance mRNAs. Quantitative real time PCR (RT-qPCR) analysis of the FEH mRNAs (including FEHI and FEHII) in cold stored roots of three chicory cultivars was studied. RT-qPCR results showed variable levels of FEHII and FEHI expression in different hgitonehis of the chicory cultivars studied. Moreover, longer cold storage of roots significantly induced expression levels of FEHII in some chicory phenotypes. These results could be used in breeding programmes to increase inulin content in root chicory by selecting genotypes based on their FEH expression profiles.},
  author       = {Maroufi, Asaf and Van Bockstaele, Erik and De Loose, Marc},
  issn         = {1835-2707},
  journal      = {AUSTRALIAN JOURNAL OF CROP SCIENCE},
  keyword      = {cold storage,quantitative real time PCR,breeding,root chicory,inulin degradation},
  language     = {eng},
  number       = {9},
  pages        = {1362--1368},
  title        = {Differential expression of fructan 1-exohydrolase genes involved in inulin biodegradation in chicory (Cichorium intybus) cultivars},
  url          = {http://www.cropj.com/maroufi\_6\_9\_2012\_1362\_1368.pdf},
  volume       = {6},
  year         = {2012},
}

Chicago
Maroufi, Asaf, Erik Van Bockstaele, and Marc De Loose. 2012. “Differential Expression of Fructan 1-exohydrolase Genes Involved in Inulin Biodegradation in Chicory (Cichorium Intybus) Cultivars.” Australian Journal of Crop Science 6 (9): 1362–1368.
APA
Maroufi, Asaf, Van Bockstaele, E., & De Loose, M. (2012). Differential expression of fructan 1-exohydrolase genes involved in inulin biodegradation in chicory (Cichorium intybus) cultivars. AUSTRALIAN JOURNAL OF CROP SCIENCE, 6(9), 1362–1368.
Vancouver
1.
Maroufi A, Van Bockstaele E, De Loose M. Differential expression of fructan 1-exohydrolase genes involved in inulin biodegradation in chicory (Cichorium intybus) cultivars. AUSTRALIAN JOURNAL OF CROP SCIENCE. 2012;6(9):1362–8.
MLA
Maroufi, Asaf, Erik Van Bockstaele, and Marc De Loose. “Differential Expression of Fructan 1-exohydrolase Genes Involved in Inulin Biodegradation in Chicory (Cichorium Intybus) Cultivars.” AUSTRALIAN JOURNAL OF CROP SCIENCE 6.9 (2012): 1362–1368. Print.