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Transcription factor Efg1 contributes to the tolerance of Candida albicans biofilms against antifungal agents in vitro and in vivo

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Abstract
We investigated the molecular basis of the tolerance of Candida albicans biofilms to antifungals using the miconazole as a model compound, and translated the resulting data to other antifungals. Sessile cells of C. albicans Delta efg1, lacking the transcription factor Efg1, showed increased susceptibility to miconazole, amphotericin B and caspofungin, whereas these sessile cells were equally resistant to fluconazole. The increased sensitivity to miconazole was, at least, partly due to an increased accumulation of miconazole in the cells as compared to wild-type or reintegrant Delta efg1(EFG1) sessile cells. By using a rat biofilm model, we further confirmed the role of Efg1 in the tolerance of C. albicans biofilms to miconazole when grown in vivo.
Keywords
REGULATOR, GENE, SUSCEPTIBILITY, RAT MODEL, SIGNALING PATHWAY, SACCHAROMYCES-CEREVISIAE, MORPHOGENESIS, MICONAZOLE, ANIDULAFUNGIN, PARAPSILOSIS

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MLA
Bink, Anna, et al. “Transcription Factor Efg1 Contributes to the Tolerance of Candida Albicans Biofilms against Antifungal Agents in Vitro and in Vivo.” JOURNAL OF MEDICAL MICROBIOLOGY, vol. 61, no. 6, 2012, pp. 813–19, doi:10.1099/jmm.0.041020-0.
APA
Bink, A., Govaert, G., Vandenbosch, D., Kuchariková, S., Coenye, T., Nelis, H., … Thevissen, K. (2012). Transcription factor Efg1 contributes to the tolerance of Candida albicans biofilms against antifungal agents in vitro and in vivo. JOURNAL OF MEDICAL MICROBIOLOGY, 61(6), 813–819. https://doi.org/10.1099/jmm.0.041020-0
Chicago author-date
Bink, Anna, Gilmer Govaert, Davy Vandenbosch, Soňa Kuchariková, Tom Coenye, Hans Nelis, Patrick Van Dijck, Bruno PA Cammue, and Karin Thevissen. 2012. “Transcription Factor Efg1 Contributes to the Tolerance of Candida Albicans Biofilms against Antifungal Agents in Vitro and in Vivo.” JOURNAL OF MEDICAL MICROBIOLOGY 61 (6): 813–19. https://doi.org/10.1099/jmm.0.041020-0.
Chicago author-date (all authors)
Bink, Anna, Gilmer Govaert, Davy Vandenbosch, Soňa Kuchariková, Tom Coenye, Hans Nelis, Patrick Van Dijck, Bruno PA Cammue, and Karin Thevissen. 2012. “Transcription Factor Efg1 Contributes to the Tolerance of Candida Albicans Biofilms against Antifungal Agents in Vitro and in Vivo.” JOURNAL OF MEDICAL MICROBIOLOGY 61 (6): 813–819. doi:10.1099/jmm.0.041020-0.
Vancouver
1.
Bink A, Govaert G, Vandenbosch D, Kuchariková S, Coenye T, Nelis H, et al. Transcription factor Efg1 contributes to the tolerance of Candida albicans biofilms against antifungal agents in vitro and in vivo. JOURNAL OF MEDICAL MICROBIOLOGY. 2012;61(6):813–9.
IEEE
[1]
A. Bink et al., “Transcription factor Efg1 contributes to the tolerance of Candida albicans biofilms against antifungal agents in vitro and in vivo,” JOURNAL OF MEDICAL MICROBIOLOGY, vol. 61, no. 6, pp. 813–819, 2012.
@article{2988522,
  abstract     = {{We investigated the molecular basis of the tolerance of Candida albicans biofilms to antifungals using the miconazole as a model compound, and translated the resulting data to other antifungals. Sessile cells of C. albicans Delta efg1, lacking the transcription factor Efg1, showed increased susceptibility to miconazole, amphotericin B and caspofungin, whereas these sessile cells were equally resistant to fluconazole. The increased sensitivity to miconazole was, at least, partly due to an increased accumulation of miconazole in the cells as compared to wild-type or reintegrant Delta efg1(EFG1) sessile cells. By using a rat biofilm model, we further confirmed the role of Efg1 in the tolerance of C. albicans biofilms to miconazole when grown in vivo.}},
  author       = {{Bink, Anna and Govaert, Gilmer and Vandenbosch, Davy and Kuchariková, Soňa and Coenye, Tom and Nelis, Hans and Van Dijck, Patrick and Cammue, Bruno PA and Thevissen, Karin}},
  issn         = {{0022-2615}},
  journal      = {{JOURNAL OF MEDICAL MICROBIOLOGY}},
  keywords     = {{REGULATOR,GENE,SUSCEPTIBILITY,RAT MODEL,SIGNALING PATHWAY,SACCHAROMYCES-CEREVISIAE,MORPHOGENESIS,MICONAZOLE,ANIDULAFUNGIN,PARAPSILOSIS}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{813--819}},
  title        = {{Transcription factor Efg1 contributes to the tolerance of Candida albicans biofilms against antifungal agents in vitro and in vivo}},
  url          = {{http://doi.org/10.1099/jmm.0.041020-0}},
  volume       = {{61}},
  year         = {{2012}},
}

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