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Structural characterization of extracellular polysaccharides of Azorhizobium caulinodans and importance for nodule initiation on Sesbania rostrata

Wim D'Haeze UGent, John Glushka, Riet De Rycke UGent, Marcella Holsters UGent and Russell W Carlson (2004) MOLECULAR MICROBIOLOGY. 52(2). p.485-500
abstract
During lateral root base nodulation, the microsymbiont Azorhizobium caulinodans enters its host plant, Sesbania rostrata, via the formation of outer cortical infection pockets, a process that is characterized by a massive production of H2O2. Infection threads guide bacteria from infection pockets towards nodule primordia. Previously, two mutants were constructed that produce lipopolysaccharides (LPSs) similar to one another but different from the wild-type LPS, and that are affected in extracellular polysaccharide (EPS) production. Mutant ORS571-X15 was blocked at the infection pocket stage and unable to produce EPS. The other mutant, ORS571-oac2, was impaired in the release from infection threads and was surrounded by a thin layer of EPS in comparison to the wild-type strain that produced massive amounts of EPS. Structural characterization revealed that EPS purified from cultured and nodule bacteria was a linear homopolysaccharide of alpha-1,3-linked 4,6-O-(1-carboxyethylidene)-D-galactosyl residues. In situ H2O2 localization demonstrated that increased EPS production during early stages of invasion prevented the incorporation of H2O2 inside the bacteria, suggesting a role for EPS in protecting the microsymbiont against H2O2. In addition, ex planta assays confirmed a positive correlation between increased EPS production and enhanced protection against H2O2.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PROTECTIVE ROLE, PLANT DEFENSE, INFECTION, MEDICAGO-SATIVA, ALFALFA NODULES, ESCHERICHIA-COLI, HYDROGEN-PEROXIDE, SINORHIZOBIUM-MELILOTI, CALCOFLUOR-BINDING EXOPOLYSACCHARIDE, RHIZOBIUM-LEGUME SYMBIOSIS
journal title
MOLECULAR MICROBIOLOGY
Mol. Microbiol.
volume
52
issue
2
pages
485 - 500
Web of Science type
Article
Web of Science id
000220624000014
JCR category
MICROBIOLOGY
JCR impact factor
5.959 (2004)
JCR rank
9/84 (2004)
JCR quartile
1 (2004)
ISSN
0950-382X
DOI
10.1111/j.1365-2958.2004.03989.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
298736
handle
http://hdl.handle.net/1854/LU-298736
date created
2005-02-17 15:38:00
date last changed
2013-10-16 14:13:12
@article{298736,
  abstract     = {During lateral root base nodulation, the microsymbiont Azorhizobium caulinodans enters its host plant, Sesbania rostrata, via the formation of outer cortical infection pockets, a process that is characterized by a massive production of H2O2. Infection threads guide bacteria from infection pockets towards nodule primordia. Previously, two mutants were constructed that produce lipopolysaccharides (LPSs) similar to one another but different from the wild-type LPS, and that are affected in extracellular polysaccharide (EPS) production. Mutant ORS571-X15 was blocked at the infection pocket stage and unable to produce EPS. The other mutant, ORS571-oac2, was impaired in the release from infection threads and was surrounded by a thin layer of EPS in comparison to the wild-type strain that produced massive amounts of EPS. Structural characterization revealed that EPS purified from cultured and nodule bacteria was a linear homopolysaccharide of alpha-1,3-linked 4,6-O-(1-carboxyethylidene)-D-galactosyl residues. In situ H2O2 localization demonstrated that increased EPS production during early stages of invasion prevented the incorporation of H2O2 inside the bacteria, suggesting a role for EPS in protecting the microsymbiont against H2O2. In addition, ex planta assays confirmed a positive correlation between increased EPS production and enhanced protection against H2O2.},
  author       = {D'Haeze, Wim and Glushka, John and De Rycke, Riet and Holsters, Marcella and Carlson, Russell W},
  issn         = {0950-382X},
  journal      = {MOLECULAR MICROBIOLOGY},
  keyword      = {PROTECTIVE ROLE,PLANT DEFENSE,INFECTION,MEDICAGO-SATIVA,ALFALFA NODULES,ESCHERICHIA-COLI,HYDROGEN-PEROXIDE,SINORHIZOBIUM-MELILOTI,CALCOFLUOR-BINDING EXOPOLYSACCHARIDE,RHIZOBIUM-LEGUME SYMBIOSIS},
  language     = {eng},
  number       = {2},
  pages        = {485--500},
  title        = {Structural characterization of extracellular polysaccharides of Azorhizobium caulinodans and importance for nodule initiation on Sesbania rostrata},
  url          = {http://dx.doi.org/10.1111/j.1365-2958.2004.03989.x},
  volume       = {52},
  year         = {2004},
}

Chicago
D’Haeze, Wim, John Glushka, Riet De Rycke, Marcella Holsters, and Russell W Carlson. 2004. “Structural Characterization of Extracellular Polysaccharides of Azorhizobium Caulinodans and Importance for Nodule Initiation on Sesbania Rostrata.” Molecular Microbiology 52 (2): 485–500.
APA
D’Haeze, W., Glushka, J., De Rycke, R., Holsters, M., & Carlson, R. W. (2004). Structural characterization of extracellular polysaccharides of Azorhizobium caulinodans and importance for nodule initiation on Sesbania rostrata. MOLECULAR MICROBIOLOGY, 52(2), 485–500.
Vancouver
1.
D’Haeze W, Glushka J, De Rycke R, Holsters M, Carlson RW. Structural characterization of extracellular polysaccharides of Azorhizobium caulinodans and importance for nodule initiation on Sesbania rostrata. MOLECULAR MICROBIOLOGY. 2004;52(2):485–500.
MLA
D’Haeze, Wim, John Glushka, Riet De Rycke, et al. “Structural Characterization of Extracellular Polysaccharides of Azorhizobium Caulinodans and Importance for Nodule Initiation on Sesbania Rostrata.” MOLECULAR MICROBIOLOGY 52.2 (2004): 485–500. Print.