Molecular dissection of plant cytokinesis and phragmoplast structure: a survey of GFP-tagged proteins
- Author
- Daniël Van Damme (UGent) , François-Yves Bouget, Kris Van Poucke (UGent) , Dirk Inzé (UGent) and Danny Geelen (UGent)
- Organization
- Abstract
- To identify molecular players implicated in cytokinesis and division plane determination, the Arabidopsis thaliana genome was explored for potential cytokinesis genes. More than 100 open reading frames were selected based on similarity to yeast and animal cytokinesis genes, cytoskeleton and polarity genes, and Nicotiana tabacum genes showing cell cycle-controlled expression. The subcellular localization of these proteins was determined by means of GFP tagging in tobacco Bright Yellow-2 cells and Arabidopsis plants. Detailed confocal microscopy identified 15 proteins targeted to distinct regions of the phragmoplast and the cell plate. EB1- and MAP65-like proteins were associated with the plus-end, the minus-end, or along the entire length of microtubules. The actin-binding protein myosin, the kinase Aurora, and a novel cell cycle protein designated T22, accumulated preferentially at the midline. EB1 and Aurora, in addition to other regulatory proteins (homologs of Mob1, Sid1, and Sid2), were targeted to the nucleus, suggesting that this organelle operates as a coordinating hub for cytokinesis.
- Keywords
- BINDING PROTEIN, NUCLEATION SITES, DIVISION, ARABIDOPSIS, GAMMA-TUBULIN, FISSION YEAST, SPINDLE POLE BODY, TOBACCO BY-2 CELLS, VIRUS MOVEMENT PROTEIN, MICROTUBULE-ASSOCIATED PROTEINS, phragmoplast, microtubule, cytokinesis, actin, cell division
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-298623
- MLA
- Van Damme, Daniël, et al. “Molecular Dissection of Plant Cytokinesis and Phragmoplast Structure: A Survey of GFP-Tagged Proteins.” PLANT JOURNAL, vol. 40, no. 3, 2004, pp. 386–98, doi:10.1111/j.1365-313X.2004.02222.x.
- APA
- Van Damme, D., Bouget, F.-Y., Van Poucke, K., Inzé, D., & Geelen, D. (2004). Molecular dissection of plant cytokinesis and phragmoplast structure: a survey of GFP-tagged proteins. PLANT JOURNAL, 40(3), 386–398. https://doi.org/10.1111/j.1365-313X.2004.02222.x
- Chicago author-date
- Van Damme, Daniël, François-Yves Bouget, Kris Van Poucke, Dirk Inzé, and Danny Geelen. 2004. “Molecular Dissection of Plant Cytokinesis and Phragmoplast Structure: A Survey of GFP-Tagged Proteins.” PLANT JOURNAL 40 (3): 386–98. https://doi.org/10.1111/j.1365-313X.2004.02222.x.
- Chicago author-date (all authors)
- Van Damme, Daniël, François-Yves Bouget, Kris Van Poucke, Dirk Inzé, and Danny Geelen. 2004. “Molecular Dissection of Plant Cytokinesis and Phragmoplast Structure: A Survey of GFP-Tagged Proteins.” PLANT JOURNAL 40 (3): 386–398. doi:10.1111/j.1365-313X.2004.02222.x.
- Vancouver
- 1.Van Damme D, Bouget F-Y, Van Poucke K, Inzé D, Geelen D. Molecular dissection of plant cytokinesis and phragmoplast structure: a survey of GFP-tagged proteins. PLANT JOURNAL. 2004;40(3):386–98.
- IEEE
- [1]D. Van Damme, F.-Y. Bouget, K. Van Poucke, D. Inzé, and D. Geelen, “Molecular dissection of plant cytokinesis and phragmoplast structure: a survey of GFP-tagged proteins,” PLANT JOURNAL, vol. 40, no. 3, pp. 386–398, 2004.
@article{298623,
abstract = {{To identify molecular players implicated in cytokinesis and division plane determination, the Arabidopsis thaliana genome was explored for potential cytokinesis genes. More than 100 open reading frames were selected based on similarity to yeast and animal cytokinesis genes, cytoskeleton and polarity genes, and Nicotiana tabacum genes showing cell cycle-controlled expression. The subcellular localization of these proteins was determined by means of GFP tagging in tobacco Bright Yellow-2 cells and Arabidopsis plants. Detailed confocal microscopy identified 15 proteins targeted to distinct regions of the phragmoplast and the cell plate. EB1- and MAP65-like proteins were associated with the plus-end, the minus-end, or along the entire length of microtubules. The actin-binding protein myosin, the kinase Aurora, and a novel cell cycle protein designated T22, accumulated preferentially at the midline. EB1 and Aurora, in addition to other regulatory proteins (homologs of Mob1, Sid1, and Sid2), were targeted to the nucleus, suggesting that this organelle operates as a coordinating hub for cytokinesis.}},
author = {{Van Damme, Daniël and Bouget, François-Yves and Van Poucke, Kris and Inzé, Dirk and Geelen, Danny}},
issn = {{0960-7412}},
journal = {{PLANT JOURNAL}},
keywords = {{BINDING PROTEIN,NUCLEATION SITES,DIVISION,ARABIDOPSIS,GAMMA-TUBULIN,FISSION YEAST,SPINDLE POLE BODY,TOBACCO BY-2 CELLS,VIRUS MOVEMENT PROTEIN,MICROTUBULE-ASSOCIATED PROTEINS,phragmoplast,microtubule,cytokinesis,actin,cell division}},
language = {{eng}},
number = {{3}},
pages = {{386--398}},
title = {{Molecular dissection of plant cytokinesis and phragmoplast structure: a survey of GFP-tagged proteins}},
url = {{http://doi.org/10.1111/j.1365-313X.2004.02222.x}},
volume = {{40}},
year = {{2004}},
}
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