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Fluorescence imaging-based forward genetic screens to identify trafficking regulators in plants

Marta Zwiewka (UGent) and Jiri Friml (UGent)
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Abstract
Coordinated, subcellular trafficking of proteins is one of the fundamental properties of the multicellular eukaryotic organisms. Trafficking involves a large diversity of compartments, pathways, cargo molecules, and vesicle-sorting events. It is also crucial in regulating the localization and, thus, the activity of various proteins, but the process is still poorly genetically defined in plants. In the past, forward genetics screens had been used to determine the function of genes by searching for a specific morphological phenotype in the organism population in which mutations had been induced chemically or by irradiation. Unfortunately, these straightforward genetic screens turned out to be limited in identifying new regulators of intracellular protein transport, because mutations affecting essential trafficking pathways often lead to lethality. In addition, the use of these approaches has been restricted by functional redundancy among trafficking regulators. Screens for mutants that rely on the observation of changes in the cellular localization or dynamics of fluorescent subcellular markers enable, at least partially, to circumvent these issues. Hence, such image-based screens provide the possibility to identify either alleles with weak effects or components of the subcellular trafficking machinery that have no strong impact on the plant growth.
Keywords
protein trafficking, plant, screening, forward genetics, fluorescent protein

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MLA
Zwiewka, Marta, and Jiri Friml. “Fluorescence Imaging-based Forward Genetic Screens to Identify Trafficking Regulators in Plants.” FRONTIERS IN PLANT SCIENCE 3 (2012): n. pag. Print.
APA
Zwiewka, M., & Friml, J. (2012). Fluorescence imaging-based forward genetic screens to identify trafficking regulators in plants. FRONTIERS IN PLANT SCIENCE, 3.
Chicago author-date
Zwiewka, Marta, and Jiri Friml. 2012. “Fluorescence Imaging-based Forward Genetic Screens to Identify Trafficking Regulators in Plants.” Frontiers in Plant Science 3.
Chicago author-date (all authors)
Zwiewka, Marta, and Jiri Friml. 2012. “Fluorescence Imaging-based Forward Genetic Screens to Identify Trafficking Regulators in Plants.” Frontiers in Plant Science 3.
Vancouver
1.
Zwiewka M, Friml J. Fluorescence imaging-based forward genetic screens to identify trafficking regulators in plants. FRONTIERS IN PLANT SCIENCE. 2012;3.
IEEE
[1]
M. Zwiewka and J. Friml, “Fluorescence imaging-based forward genetic screens to identify trafficking regulators in plants,” FRONTIERS IN PLANT SCIENCE, vol. 3, 2012.
@article{2985650,
  abstract     = {Coordinated, subcellular trafficking of proteins is one of the fundamental properties of the multicellular eukaryotic organisms. Trafficking involves a large diversity of compartments, pathways, cargo molecules, and vesicle-sorting events. It is also crucial in regulating the localization and, thus, the activity of various proteins, but the process is still poorly genetically defined in plants. In the past, forward genetics screens had been used to determine the function of genes by searching for a specific morphological phenotype in the organism population in which mutations had been induced chemically or by irradiation. Unfortunately, these straightforward genetic screens turned out to be limited in identifying new regulators of intracellular protein transport, because mutations affecting essential trafficking pathways often lead to lethality. In addition, the use of these approaches has been restricted by functional redundancy among trafficking regulators. Screens for mutants that rely on the observation of changes in the cellular localization or dynamics of fluorescent subcellular markers enable, at least partially, to circumvent these issues. Hence, such image-based screens provide the possibility to identify either alleles with weak effects or components of the subcellular trafficking machinery that have no strong impact on the plant growth.},
  articleno    = {97},
  author       = {Zwiewka, Marta and Friml, Jiri},
  issn         = {1664-462X},
  journal      = {FRONTIERS IN PLANT SCIENCE},
  keywords     = {protein trafficking,plant,screening,forward genetics,fluorescent protein},
  language     = {eng},
  pages        = {14},
  title        = {Fluorescence imaging-based forward genetic screens to identify trafficking regulators in plants},
  url          = {http://dx.doi.org/10.3389/fpls.2012.00097},
  volume       = {3},
  year         = {2012},
}

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