Advanced search
1 file | 326.18 KB Add to list

Isolation and characterisation of an equol-producing mixed microbial culture from a human faecal sample and its activity under gastrointestinal conditions

(2005) ARCHIVES OF MICROBIOLOGY. 183(1). p.45-55
Author
Organization
Abstract
Only about one third of humans possess a microbiota capable of transforming the dietary isoflavone daidzein into equol. Little is known about the dietary and physiological factors determining this ecological feature. In this study, the in vitro metabolism of daidzein by faecal samples from four human individuals was investigated. One culture produced the metabolites dihydrodaidzein and O-desmethylangolensin, another produced dihydrodaidzein and equol. From the latter, a stable and transferable mixed culture transforming daidzein into equol was obtained. Molecular fingerprinting analysis (denaturing gradient gel electrophoresis) showed the presence of four bacterial species of which only the first three strains could be brought into pure culture. These strains were identified as Lactobacillus mucosae EPI2, Enterococcus faecium EPI1 and Finegoldia magna EPI3, and did not produce equol in pure culture. The fourth species was tentatively identified as Veillonella sp strain EP. It was found that hydrogen gas in particular, but also butyrate and propionate, which are all colonic fermentation products from poorly digestible carbohydrates, stimulated equol production by the mixed culture. However, when fructo-oligosaccharides were added, equol production was inhibited. Furthermore, the equol-producing capacity of the isolated culture was maintained upon its addition to a faecal culture originating from a non-equol-producing individual.
Keywords
equol, isoflavone, hydrogen gas, bacteria, FOS, ESTROGEN-RECEPTOR-ALPHA, IN-VITRO, ISOFLAVONE GLYCOSIDES, PHYTO-ESTROGENS, SOY ISOFLAVONES, GUT MICROFLORA, SOYBEAN FOODS, PREMENOPAUSAL WOMEN, INTESTINAL BACTERIA, C-RING

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 326.18 KB

Citation

Please use this url to cite or link to this publication:

MLA
Decroos, Karel, Steffi Vanhemmens, Sofie Cattoir, et al. “Isolation and Characterisation of an Equol-producing Mixed Microbial Culture from a Human Faecal Sample and Its Activity Under Gastrointestinal Conditions.” ARCHIVES OF MICROBIOLOGY 183.1 (2005): 45–55. Print.
APA
Decroos, Karel, Vanhemmens, S., Cattoir, S., Boon, N., & Verstraete, W. (2005). Isolation and characterisation of an equol-producing mixed microbial culture from a human faecal sample and its activity under gastrointestinal conditions. ARCHIVES OF MICROBIOLOGY, 183(1), 45–55.
Chicago author-date
Decroos, Karel, Steffi Vanhemmens, Sofie Cattoir, Nico Boon, and Willy Verstraete. 2005. “Isolation and Characterisation of an Equol-producing Mixed Microbial Culture from a Human Faecal Sample and Its Activity Under Gastrointestinal Conditions.” Archives of Microbiology 183 (1): 45–55.
Chicago author-date (all authors)
Decroos, Karel, Steffi Vanhemmens, Sofie Cattoir, Nico Boon, and Willy Verstraete. 2005. “Isolation and Characterisation of an Equol-producing Mixed Microbial Culture from a Human Faecal Sample and Its Activity Under Gastrointestinal Conditions.” Archives of Microbiology 183 (1): 45–55.
Vancouver
1.
Decroos K, Vanhemmens S, Cattoir S, Boon N, Verstraete W. Isolation and characterisation of an equol-producing mixed microbial culture from a human faecal sample and its activity under gastrointestinal conditions. ARCHIVES OF MICROBIOLOGY. 2005;183(1):45–55.
IEEE
[1]
K. Decroos, S. Vanhemmens, S. Cattoir, N. Boon, and W. Verstraete, “Isolation and characterisation of an equol-producing mixed microbial culture from a human faecal sample and its activity under gastrointestinal conditions,” ARCHIVES OF MICROBIOLOGY, vol. 183, no. 1, pp. 45–55, 2005.
@article{298426,
  abstract     = {{Only about one third of humans possess a microbiota capable of transforming the dietary isoflavone daidzein into equol. Little is known about the dietary and physiological factors determining this ecological feature. In this study, the in vitro metabolism of daidzein by faecal samples from four human individuals was investigated. One culture produced the metabolites dihydrodaidzein and O-desmethylangolensin, another produced dihydrodaidzein and equol. From the latter, a stable and transferable mixed culture transforming daidzein into equol was obtained. Molecular fingerprinting analysis (denaturing gradient gel electrophoresis) showed the presence of four bacterial species of which only the first three strains could be brought into pure culture. These strains were identified as Lactobacillus mucosae EPI2, Enterococcus faecium EPI1 and Finegoldia magna EPI3, and did not produce equol in pure culture. The fourth species was tentatively identified as Veillonella sp strain EP. It was found that hydrogen gas in particular, but also butyrate and propionate, which are all colonic fermentation products from poorly digestible carbohydrates, stimulated equol production by the mixed culture. However, when fructo-oligosaccharides were added, equol production was inhibited. Furthermore, the equol-producing capacity of the isolated culture was maintained upon its addition to a faecal culture originating from a non-equol-producing individual.}},
  author       = {{Decroos, Karel and Vanhemmens, Steffi and Cattoir, Sofie and Boon, Nico and Verstraete, Willy}},
  issn         = {{0302-8933}},
  journal      = {{ARCHIVES OF MICROBIOLOGY}},
  keywords     = {{equol,isoflavone,hydrogen gas,bacteria,FOS,ESTROGEN-RECEPTOR-ALPHA,IN-VITRO,ISOFLAVONE GLYCOSIDES,PHYTO-ESTROGENS,SOY ISOFLAVONES,GUT MICROFLORA,SOYBEAN FOODS,PREMENOPAUSAL WOMEN,INTESTINAL BACTERIA,C-RING}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{45--55}},
  title        = {{Isolation and characterisation of an equol-producing mixed microbial culture from a human faecal sample and its activity under gastrointestinal conditions}},
  url          = {{http://dx.doi.org/10.1007/s00203-004-0747-4}},
  volume       = {{183}},
  year         = {{2005}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: