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Quantum dot based rapid tests for zearalenone detection

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Center for nano- and biophotonics (NB-Photonics)
Abstract
Three different kinds of immunosorbent assays with luminescence detection were developed for the determination of zearalenone (ZEN), a secondary toxic metabolite of Fusarium fungi. CdSe/ZnS core/shell quantum dots (QDs) were used as a label in quantitative micro-well plate immunoassays (fluorescent-labeled immunosorbent assay, FLISA) and in qualitative column test methods. As carriers for QD-based column tests, sepharose gel (for covalent binding of antibody) and polyethylene frits (for physical absorption of antibody) were used and compared. The application of QDs as a label resulted in a fourfold decrease in the IC50 value with FLISA (0.1 ng mL(-1)) with a detection limit of 0.03 ng mL(-1) when compared with the traditional immunosorbent assay which makes use of horseradish peroxidase as the enzyme label. The cutoff levels for both qualitative column test methods were selected based on the maximum level for ZEN in unprocessed cereals established by the European Commission (100 mu g kg(-1)) as 5 ng mL(-1) taking into account extraction and dilution. The different developed immumoassays were tested for ZEN determination in raw wheat samples. As a confirmatory method, liquid chromatography coupled to tandem mass spectrometry was used. The obtained results allow using FLISA and both qualitative column test methods for the analysis of analytes with very low established maximum limits, even in very complicated food matrices, owing to the high dilution of the sample extract.
Keywords
Immunoassay, Quantum dots, On-site method, Enzyme-linked immunoassay, Fluorescent-labeled immunoassay, Zearalenone, PERFORMANCE LIQUID-CHROMATOGRAPHY, PHOSPHINE-FREE SYNTHESIS, BOVINE SERUM-ALBUMIN, FLUORESCENCE IMMUNOASSAY, CORE/SHELL NANOCRYSTALS, SENSITIVE DETECTION, CDSE NANOCRYSTALS, TEST STRIP, WATER, FLUOROIMMUNOASSAY

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Citation

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Chicago
Beloglazova, NV, ES Speranskaya, Sarah De Saeger, Zeger Hens, Sofie Abé, and IY Goryacheva. 2012. “Quantum Dot Based Rapid Tests for Zearalenone Detection.” Analytical and Bioanalytical Chemistry 403 (10): 3013–3024.
APA
Beloglazova, NV, Speranskaya, E., De Saeger, S., Hens, Z., Abé, S., & Goryacheva, I. (2012). Quantum dot based rapid tests for zearalenone detection. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 403(10), 3013–3024.
Vancouver
1.
Beloglazova N, Speranskaya E, De Saeger S, Hens Z, Abé S, Goryacheva I. Quantum dot based rapid tests for zearalenone detection. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2012;403(10):3013–24.
MLA
Beloglazova, NV, ES Speranskaya, Sarah De Saeger, et al. “Quantum Dot Based Rapid Tests for Zearalenone Detection.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 403.10 (2012): 3013–3024. Print.
@article{2979402,
  abstract     = {Three different kinds of immunosorbent assays with luminescence detection were developed for the determination of zearalenone (ZEN), a secondary toxic metabolite of Fusarium fungi. CdSe/ZnS core/shell quantum dots (QDs) were used as a label in quantitative micro-well plate immunoassays (fluorescent-labeled immunosorbent assay, FLISA) and in qualitative column test methods. As carriers for QD-based column tests, sepharose gel (for covalent binding of antibody) and polyethylene frits (for physical absorption of antibody) were used and compared. The application of QDs as a label resulted in a fourfold decrease in the IC50 value with FLISA (0.1 ng mL(-1)) with a detection limit of 0.03 ng mL(-1) when compared with the traditional immunosorbent assay which makes use of horseradish peroxidase as the enzyme label. The cutoff levels for both qualitative column test methods were selected based on the maximum level for ZEN in unprocessed cereals established by the European Commission (100 mu g kg(-1)) as 5 ng mL(-1) taking into account extraction and dilution. The different developed immumoassays were tested for ZEN determination in raw wheat samples. As a confirmatory method, liquid chromatography coupled to tandem mass spectrometry was used. The obtained results allow using FLISA and both qualitative column test methods for the analysis of analytes with very low established maximum limits, even in very complicated food matrices, owing to the high dilution of the sample extract.},
  author       = {Beloglazova, NV and Speranskaya, ES and De Saeger, Sarah and Hens, Zeger and Ab{\'e}, Sofie and Goryacheva, IY},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keyword      = {Immunoassay,Quantum dots,On-site method,Enzyme-linked immunoassay,Fluorescent-labeled immunoassay,Zearalenone,PERFORMANCE LIQUID-CHROMATOGRAPHY,PHOSPHINE-FREE SYNTHESIS,BOVINE SERUM-ALBUMIN,FLUORESCENCE IMMUNOASSAY,CORE/SHELL NANOCRYSTALS,SENSITIVE DETECTION,CDSE NANOCRYSTALS,TEST STRIP,WATER,FLUOROIMMUNOASSAY},
  language     = {eng},
  number       = {10},
  pages        = {3013--3024},
  title        = {Quantum dot based rapid tests for zearalenone detection},
  url          = {http://dx.doi.org/10.1007/s00216-012-5981-z},
  volume       = {403},
  year         = {2012},
}

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