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Quantum dot based rapid tests for zearalenone detection

NV Beloglazova, ES Speranskaya, Sarah De Saeger UGent, Zeger Hens UGent, Sofie Abé UGent and IY Goryacheva (2012) ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 403(10). p.3013-3024
abstract
Three different kinds of immunosorbent assays with luminescence detection were developed for the determination of zearalenone (ZEN), a secondary toxic metabolite of Fusarium fungi. CdSe/ZnS core/shell quantum dots (QDs) were used as a label in quantitative micro-well plate immunoassays (fluorescent-labeled immunosorbent assay, FLISA) and in qualitative column test methods. As carriers for QD-based column tests, sepharose gel (for covalent binding of antibody) and polyethylene frits (for physical absorption of antibody) were used and compared. The application of QDs as a label resulted in a fourfold decrease in the IC50 value with FLISA (0.1 ng mL(-1)) with a detection limit of 0.03 ng mL(-1) when compared with the traditional immunosorbent assay which makes use of horseradish peroxidase as the enzyme label. The cutoff levels for both qualitative column test methods were selected based on the maximum level for ZEN in unprocessed cereals established by the European Commission (100 mu g kg(-1)) as 5 ng mL(-1) taking into account extraction and dilution. The different developed immumoassays were tested for ZEN determination in raw wheat samples. As a confirmatory method, liquid chromatography coupled to tandem mass spectrometry was used. The obtained results allow using FLISA and both qualitative column test methods for the analysis of analytes with very low established maximum limits, even in very complicated food matrices, owing to the high dilution of the sample extract.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Immunoassay, Quantum dots, On-site method, Enzyme-linked immunoassay, Fluorescent-labeled immunoassay, Zearalenone, PERFORMANCE LIQUID-CHROMATOGRAPHY, PHOSPHINE-FREE SYNTHESIS, BOVINE SERUM-ALBUMIN, FLUORESCENCE IMMUNOASSAY, CORE/SHELL NANOCRYSTALS, SENSITIVE DETECTION, CDSE NANOCRYSTALS, TEST STRIP, WATER, FLUOROIMMUNOASSAY
journal title
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Anal. Bioanal. Chem.
volume
403
issue
10
pages
3013 - 3024
Web of Science type
Article
Web of Science id
000305519400024
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.659 (2012)
JCR rank
9/74 (2012)
JCR quartile
1 (2012)
ISSN
1618-2642
DOI
10.1007/s00216-012-5981-z
project
Center for nano- and biophotonics (NB-Photonics)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2979402
handle
http://hdl.handle.net/1854/LU-2979402
date created
2012-09-05 17:38:38
date last changed
2015-06-17 10:13:47
@article{2979402,
  abstract     = {Three different kinds of immunosorbent assays with luminescence detection were developed for the determination of zearalenone (ZEN), a secondary toxic metabolite of Fusarium fungi. CdSe/ZnS core/shell quantum dots (QDs) were used as a label in quantitative micro-well plate immunoassays (fluorescent-labeled immunosorbent assay, FLISA) and in qualitative column test methods. As carriers for QD-based column tests, sepharose gel (for covalent binding of antibody) and polyethylene frits (for physical absorption of antibody) were used and compared. The application of QDs as a label resulted in a fourfold decrease in the IC50 value with FLISA (0.1 ng mL(-1)) with a detection limit of 0.03 ng mL(-1) when compared with the traditional immunosorbent assay which makes use of horseradish peroxidase as the enzyme label. The cutoff levels for both qualitative column test methods were selected based on the maximum level for ZEN in unprocessed cereals established by the European Commission (100 mu g kg(-1)) as 5 ng mL(-1) taking into account extraction and dilution. The different developed immumoassays were tested for ZEN determination in raw wheat samples. As a confirmatory method, liquid chromatography coupled to tandem mass spectrometry was used. The obtained results allow using FLISA and both qualitative column test methods for the analysis of analytes with very low established maximum limits, even in very complicated food matrices, owing to the high dilution of the sample extract.},
  author       = {Beloglazova, NV and Speranskaya, ES and De Saeger, Sarah and Hens, Zeger and Ab{\'e}, Sofie and Goryacheva, IY},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keyword      = {Immunoassay,Quantum dots,On-site method,Enzyme-linked immunoassay,Fluorescent-labeled immunoassay,Zearalenone,PERFORMANCE LIQUID-CHROMATOGRAPHY,PHOSPHINE-FREE SYNTHESIS,BOVINE SERUM-ALBUMIN,FLUORESCENCE IMMUNOASSAY,CORE/SHELL NANOCRYSTALS,SENSITIVE DETECTION,CDSE NANOCRYSTALS,TEST STRIP,WATER,FLUOROIMMUNOASSAY},
  language     = {eng},
  number       = {10},
  pages        = {3013--3024},
  title        = {Quantum dot based rapid tests for zearalenone detection},
  url          = {http://dx.doi.org/10.1007/s00216-012-5981-z},
  volume       = {403},
  year         = {2012},
}

Chicago
Beloglazova, NV, ES Speranskaya, Sarah De Saeger, Zeger Hens, Sofie Abé, and IY Goryacheva. 2012. “Quantum Dot Based Rapid Tests for Zearalenone Detection.” Analytical and Bioanalytical Chemistry 403 (10): 3013–3024.
APA
Beloglazova, NV, Speranskaya, E., De Saeger, S., Hens, Z., Abé, S., & Goryacheva, I. (2012). Quantum dot based rapid tests for zearalenone detection. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 403(10), 3013–3024.
Vancouver
1.
Beloglazova N, Speranskaya E, De Saeger S, Hens Z, Abé S, Goryacheva I. Quantum dot based rapid tests for zearalenone detection. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2012;403(10):3013–24.
MLA
Beloglazova, NV, ES Speranskaya, Sarah De Saeger, et al. “Quantum Dot Based Rapid Tests for Zearalenone Detection.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 403.10 (2012): 3013–3024. Print.