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A ROP GTPase-dependent auxin signaling pathway regulates the subcellular distribution of PIN2 in Arabidopsis roots

Deshu Lin, Shingo Nagawa, Jisheng Chen, Lingyan Cao, Xu Chen UGent, Tongda Xu, Hongjiang Li, Pankaj Dhonukshe UGent, Chizuko Yamamuro and Jiri Friml UGent, et al. (2012) CURRENT BIOLOGY. 22(14). p.1319-1325
abstract
PIN-FORMED (PIN) protein-mediated auxin polar transport is critically important for development, pattern formation, and morphogenesis in plants. Auxin has been implicated in the regulation of polar auxin transport by inhibiting PIN endocytosis [1, 2], but how auxin regulates this process is poorly understood. Our genetic screen identified the Arabidopsis SPIKE1 (SPK1) gene whose loss-of-function mutations increased lateral root density and retarded gravitropic responses, as do pin2 knockout mutations [3]. SPK1 belongs to the conserved DHR2-Dock family of Rho guanine nucleotide exchange factors [4-6]. The spk1 mutations induced PIN2 internalization that was not suppressed by auxin, as did the loss-of-function mutations for Rho-like GTPase from Plants 6 (ROP6)-GTPase or its effector RIC1. Furthermore, SPK1 was required for auxin induction of ROP6 activation. Our results have established a Rho GTPase-based auxin signaling pathway that maintains PIN2 polar distribution to the plasma membrane via inhibition of its internalization in Arabidopsis roots. Our findings provide new insights into signaling mechanisms that underlie the regulation of the dynamic trafficking of PINs required for long-distance auxin transport and that link auxin signaling to PIN-mediated pattern formation and morphogenesis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
EFFLUX, CELL MORPHOGENESIS, PLANT DEVELOPMENT, ENDOCYTOSIS, TRANSPORT, GROWTH, GRAVITROPISM, POLARITY, THALIANA, SHAPE
journal title
CURRENT BIOLOGY
Curr. Biol.
volume
22
issue
14
pages
1319 - 1325
Web of Science type
Article
Web of Science id
000306823700022
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
9.494 (2012)
JCR rank
19/288 (2012)
JCR quartile
1 (2012)
ISSN
0960-9822
DOI
10.1016/j.cub.2012.05.019
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2976925
handle
http://hdl.handle.net/1854/LU-2976925
date created
2012-08-30 13:30:13
date last changed
2013-09-17 11:14:56
@article{2976925,
  abstract     = {PIN-FORMED (PIN) protein-mediated auxin polar transport is critically important for development, pattern formation, and morphogenesis in plants. Auxin has been implicated in the regulation of polar auxin transport by inhibiting PIN endocytosis [1, 2], but how auxin regulates this process is poorly understood. Our genetic screen identified the Arabidopsis SPIKE1 (SPK1) gene whose loss-of-function mutations increased lateral root density and retarded gravitropic responses, as do pin2 knockout mutations [3]. SPK1 belongs to the conserved DHR2-Dock family of Rho guanine nucleotide exchange factors [4-6]. The spk1 mutations induced PIN2 internalization that was not suppressed by auxin, as did the loss-of-function mutations for Rho-like GTPase from Plants 6 (ROP6)-GTPase or its effector RIC1. Furthermore, SPK1 was required for auxin induction of ROP6 activation. Our results have established a Rho GTPase-based auxin signaling pathway that maintains PIN2 polar distribution to the plasma membrane via inhibition of its internalization in Arabidopsis roots. Our findings provide new insights into signaling mechanisms that underlie the regulation of the dynamic trafficking of PINs required for long-distance auxin transport and that link auxin signaling to PIN-mediated pattern formation and morphogenesis.},
  author       = {Lin, Deshu and Nagawa, Shingo and Chen, Jisheng and Cao, Lingyan and Chen, Xu and Xu, Tongda and Li, Hongjiang and Dhonukshe, Pankaj and Yamamuro, Chizuko and Friml, Jiri and Scheres, Ben and Fu, Ying and Yang, Zhenbia},
  issn         = {0960-9822},
  journal      = {CURRENT BIOLOGY},
  keyword      = {EFFLUX,CELL MORPHOGENESIS,PLANT DEVELOPMENT,ENDOCYTOSIS,TRANSPORT,GROWTH,GRAVITROPISM,POLARITY,THALIANA,SHAPE},
  language     = {eng},
  number       = {14},
  pages        = {1319--1325},
  title        = {A ROP GTPase-dependent auxin signaling pathway regulates the subcellular distribution of PIN2 in Arabidopsis roots},
  url          = {http://dx.doi.org/10.1016/j.cub.2012.05.019},
  volume       = {22},
  year         = {2012},
}

Chicago
Lin, Deshu, Shingo Nagawa, Jisheng Chen, Lingyan Cao, Xu Chen, Tongda Xu, Hongjiang Li, et al. 2012. “A ROP GTPase-dependent Auxin Signaling Pathway Regulates the Subcellular Distribution of PIN2 in Arabidopsis Roots.” Current Biology 22 (14): 1319–1325.
APA
Lin, Deshu, Nagawa, S., Chen, J., Cao, L., Chen, X., Xu, T., Li, H., et al. (2012). A ROP GTPase-dependent auxin signaling pathway regulates the subcellular distribution of PIN2 in Arabidopsis roots. CURRENT BIOLOGY, 22(14), 1319–1325.
Vancouver
1.
Lin D, Nagawa S, Chen J, Cao L, Chen X, Xu T, et al. A ROP GTPase-dependent auxin signaling pathway regulates the subcellular distribution of PIN2 in Arabidopsis roots. CURRENT BIOLOGY. 2012;22(14):1319–25.
MLA
Lin, Deshu, Shingo Nagawa, Jisheng Chen, et al. “A ROP GTPase-dependent Auxin Signaling Pathway Regulates the Subcellular Distribution of PIN2 in Arabidopsis Roots.” CURRENT BIOLOGY 22.14 (2012): 1319–1325. Print.