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Brachypodium distachyon promoters as efficient building blocks for transgenic research in maize

Griet Coussens UGent, Stijn Aesaert UGent, Wim Verelst UGent, Marlies Demeulenaere UGent, Sylvie De Buck UGent, Elizabeth Njuguna, Dirk Inzé UGent and Maria Van Lijsebettens UGent (2012) JOURNAL OF EXPERIMENTAL BOTANY. 63(11). p.4263-4273
abstract
The biotechnological approach to improve performance or yield of crops or for engineering metabolic pathways requires the expression of a number of transgenes, each with a specific promoter to avoid induction of silencing mechanisms. In maize (Zea mays), used as a model for cereals, an efficient Agrobacterium tumefaciens-mediated transformation system has been established that is applied for translational research. In the current transformation vectors, the promoters of the 35S gene of the cauliflower mosaic virus and of the ubiquitin gene of maize are often used to drive the bialaphos-selectable marker and the transgene, respectively. To expand the number of promoters, genes with either constitutive or seed-specific expression were selected in Brachypodium distachyon, a model grass distantly related to maize. After the corresponding Brachypodium promoters had been fused to the beta-glucuronidase reporter gene, their activity was followed throughout maize development and quantified in a fluorimetric assay with the 4-methylumbelliferyl beta-D-glucuronide substrate. The promoters pBdEF1 alpha and pBdUBI10 were constitutively and highly active in maize, whereas pBdGLU1 was clearly endosperm-specific, hence, expanding the toolbox for transgene analysis in maize. The data indicate that Brachypodium is an excellent resource for promoters for transgenic research in heterologous cereal species.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
REGENERATION, ENDOSPERM, MEDIATED TRANSFORMATION, BETA-GLUCURONIDASE, GENE-EXPRESSION, INBRED LINES, PLANTS, PHOSPHINOTHRICIN, ELECTROPORATION, AGROBACTERIUM-TUMEFACIENS, plant development, Zea mays, Gateway vector, constitutive or endosperm-specific promoter, Brachypodium distachyon, beta-glucuronidase
journal title
JOURNAL OF EXPERIMENTAL BOTANY
J. Exp. Bot.
volume
63
issue
11
pages
4263 - 4273
Web of Science type
Article
Web of Science id
000306686900023
JCR category
PLANT SCIENCES
JCR impact factor
5.242 (2012)
JCR rank
13/193 (2012)
JCR quartile
1 (2012)
ISSN
0022-0957
DOI
10.1093/jxb/ers113
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2976911
handle
http://hdl.handle.net/1854/LU-2976911
date created
2012-08-30 13:10:18
date last changed
2014-05-26 10:04:42
@article{2976911,
  abstract     = {The biotechnological approach to improve performance or yield of crops or for engineering metabolic pathways requires the expression of a number of transgenes, each with a specific promoter to avoid induction of silencing mechanisms. In maize (Zea mays), used as a model for cereals, an efficient Agrobacterium tumefaciens-mediated transformation system has been established that is applied for translational research. In the current transformation vectors, the promoters of the 35S gene of the cauliflower mosaic virus and of the ubiquitin gene of maize are often used to drive the bialaphos-selectable marker and the transgene, respectively. To expand the number of promoters, genes with either constitutive or seed-specific expression were selected in Brachypodium distachyon, a model grass distantly related to maize. After the corresponding Brachypodium promoters had been fused to the beta-glucuronidase reporter gene, their activity was followed throughout maize development and quantified in a fluorimetric assay with the 4-methylumbelliferyl beta-D-glucuronide substrate. The promoters pBdEF1 alpha and pBdUBI10 were constitutively and highly active in maize, whereas pBdGLU1 was clearly endosperm-specific, hence, expanding the toolbox for transgene analysis in maize. The data indicate that Brachypodium is an excellent resource for promoters for transgenic research in heterologous cereal species.},
  author       = {Coussens, Griet and Aesaert, Stijn and Verelst, Wim and Demeulenaere, Marlies and De Buck, Sylvie and Njuguna, Elizabeth and Inz{\'e}, Dirk and Van Lijsebettens, Maria},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  keyword      = {REGENERATION,ENDOSPERM,MEDIATED TRANSFORMATION,BETA-GLUCURONIDASE,GENE-EXPRESSION,INBRED LINES,PLANTS,PHOSPHINOTHRICIN,ELECTROPORATION,AGROBACTERIUM-TUMEFACIENS,plant development,Zea mays,Gateway vector,constitutive or endosperm-specific promoter,Brachypodium distachyon,beta-glucuronidase},
  language     = {eng},
  number       = {11},
  pages        = {4263--4273},
  title        = {Brachypodium distachyon promoters as efficient building blocks for transgenic research in maize},
  url          = {http://dx.doi.org/10.1093/jxb/ers113},
  volume       = {63},
  year         = {2012},
}

Chicago
Coussens, Griet, Stijn Aesaert, Wim Verelst, Marlies Demeulenaere, Sylvie De Buck, Elizabeth Njuguna, Dirk Inzé, and Maria Van Lijsebettens. 2012. “Brachypodium Distachyon Promoters as Efficient Building Blocks for Transgenic Research in Maize.” Journal of Experimental Botany 63 (11): 4263–4273.
APA
Coussens, G., Aesaert, S., Verelst, W., Demeulenaere, M., De Buck, S., Njuguna, E., Inzé, D., et al. (2012). Brachypodium distachyon promoters as efficient building blocks for transgenic research in maize. JOURNAL OF EXPERIMENTAL BOTANY, 63(11), 4263–4273.
Vancouver
1.
Coussens G, Aesaert S, Verelst W, Demeulenaere M, De Buck S, Njuguna E, et al. Brachypodium distachyon promoters as efficient building blocks for transgenic research in maize. JOURNAL OF EXPERIMENTAL BOTANY. 2012;63(11):4263–73.
MLA
Coussens, Griet, Stijn Aesaert, Wim Verelst, et al. “Brachypodium Distachyon Promoters as Efficient Building Blocks for Transgenic Research in Maize.” JOURNAL OF EXPERIMENTAL BOTANY 63.11 (2012): 4263–4273. Print.