Ghent University Academic Bibliography

Advanced

Arginase-1-independent polyamine production stimulates the expression of IL-4-induced alternatively activated macrophage markers while inhibiting LPS-induced expression of inflammatory genes

Jan Van den Bossche, Wouter H Lamers, Eleonorre S Koehler, Jan MC Geuns, Leena Alhonen, Anne Uimari, Sini Pirnes-Karhu, Eva Van Overmeire, Yannick Morias and Lea Brys, et al. (2012) JOURNAL OF LEUKOCYTE BIOLOGY. 91(5). p.685-699
abstract
In macrophages, basal polyamine (putrescine, spermidine, and spermine) levels are relatively low but are increased upon IL-4 stimulation. This Th2 cytokine induces Arg1 activity, which converts arginine into ornithine, and ornithine can be decarboxylated by ODC to produce putrescine, which is further converted into spermidine and spermine. Recently, we proposed polyamines as novel agents in IL-4-dependent E-cadherin regulation in AAMs. Here, we demonstrate for the first time that several, but not all, AAM markers depend on polyamines for their IL-4-induced gene and protein expression and that polyamine dependency of genes relies on the macrophage type. Remarkably, Arg1-deficient macrophages display rather enhanced IL-4-induced polyamine production, suggesting that an Arg1-independent polyamine synthesis pathway may operate in macrophages. On the other side of the macrophage activation spectrum, LPS-induced expression of several proinflammatory genes was increased significantly in polyamine-depleted CAMs. Overall, we propose Arg1 independently produced polyamines as novel regulators of the inflammatory status of the macrophage. Indeed, whereas polyamines are needed for IL-4-induced expression of several AAM mediators, they inhibit the LPS-mediated expression of proinflammatory genes in CAMs.
Please use this url to cite or link to this publication:
author
organization
alternative title
Pivotal advance: Arginase-1-independent polyamine production stimulates the expression of IL-4-induced alternatively activated macrophage markers while inhibiting LPS-induced expression of inflammatory genes
year
type
journalArticle (original)
publication status
published
subject
keyword
LPS-regulated genes, TRANSGENIC MICE, NITRIC-OXIDE SYNTHASE, IN-VIVO, DIFFERENTIAL EXPRESSION, ORNITHINE-DECARBOXYLASE, OVEREXPRESSING SPERMIDINE/SPERMINE N-1-ACETYLTRANSFERASE, M2, AAM, classically activated macrophage (CAM or M1), PERFORMANCE LIQUID-CHROMATOGRAPHY, IL-4-regulated genes, ARGININE METABOLISM, ANTIZYME INHIBITOR, INTERFERON-GAMMA
journal title
JOURNAL OF LEUKOCYTE BIOLOGY
J. Leukoc. Biol.
volume
91
issue
5
pages
685 - 699
Web of Science type
Article
Web of Science id
000303837200004
JCR category
HEMATOLOGY
JCR impact factor
4.568 (2012)
JCR rank
14/66 (2012)
JCR quartile
1 (2012)
ISSN
0741-5400
DOI
10.1189/jlb.0911453
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2976305
handle
http://hdl.handle.net/1854/LU-2976305
date created
2012-08-29 12:45:10
date last changed
2012-09-05 12:03:47
@article{2976305,
  abstract     = {In macrophages, basal polyamine (putrescine, spermidine, and spermine) levels are relatively low but are increased upon IL-4 stimulation. This Th2 cytokine induces Arg1 activity, which converts arginine into ornithine, and ornithine can be decarboxylated by ODC to produce putrescine, which is further converted into spermidine and spermine. Recently, we proposed polyamines as novel agents in IL-4-dependent E-cadherin regulation in AAMs. Here, we demonstrate for the first time that several, but not all, AAM markers depend on polyamines for their IL-4-induced gene and protein expression and that polyamine dependency of genes relies on the macrophage type. Remarkably, Arg1-deficient macrophages display rather enhanced IL-4-induced polyamine production, suggesting that an Arg1-independent polyamine synthesis pathway may operate in macrophages. On the other side of the macrophage activation spectrum, LPS-induced expression of several proinflammatory genes was increased significantly in polyamine-depleted CAMs. Overall, we propose Arg1 independently produced polyamines as novel regulators of the inflammatory status of the macrophage. Indeed, whereas polyamines are needed for IL-4-induced expression of several AAM mediators, they inhibit the LPS-mediated expression of proinflammatory genes in CAMs.},
  author       = {Van den Bossche, Jan and Lamers, Wouter H and Koehler, Eleonorre S and Geuns, Jan MC and Alhonen, Leena and Uimari, Anne and Pirnes-Karhu, Sini and Van Overmeire, Eva and Morias, Yannick and Brys, Lea and Vereecke, Lars and De Baetselier, Patrick and Van Ginderachter, Jo A},
  issn         = {0741-5400},
  journal      = {JOURNAL OF LEUKOCYTE BIOLOGY},
  keyword      = {LPS-regulated genes,TRANSGENIC MICE,NITRIC-OXIDE SYNTHASE,IN-VIVO,DIFFERENTIAL EXPRESSION,ORNITHINE-DECARBOXYLASE,OVEREXPRESSING SPERMIDINE/SPERMINE N-1-ACETYLTRANSFERASE,M2,AAM,classically activated macrophage (CAM or M1),PERFORMANCE LIQUID-CHROMATOGRAPHY,IL-4-regulated genes,ARGININE METABOLISM,ANTIZYME INHIBITOR,INTERFERON-GAMMA},
  language     = {eng},
  number       = {5},
  pages        = {685--699},
  title        = {Arginase-1-independent polyamine production stimulates the expression of IL-4-induced alternatively activated macrophage markers while inhibiting LPS-induced expression of inflammatory genes},
  url          = {http://dx.doi.org/10.1189/jlb.0911453},
  volume       = {91},
  year         = {2012},
}

Chicago
Van den Bossche, Jan, Wouter H Lamers, Eleonorre S Koehler, Jan MC Geuns, Leena Alhonen, Anne Uimari, Sini Pirnes-Karhu, et al. 2012. “Arginase-1-independent Polyamine Production Stimulates the Expression of IL-4-induced Alternatively Activated Macrophage Markers While Inhibiting LPS-induced Expression of Inflammatory Genes.” Journal of Leukocyte Biology 91 (5): 685–699.
APA
Van den Bossche, Jan, Lamers, W. H., Koehler, E. S., Geuns, J. M., Alhonen, L., Uimari, A., Pirnes-Karhu, S., et al. (2012). Arginase-1-independent polyamine production stimulates the expression of IL-4-induced alternatively activated macrophage markers while inhibiting LPS-induced expression of inflammatory genes. JOURNAL OF LEUKOCYTE BIOLOGY, 91(5), 685–699.
Vancouver
1.
Van den Bossche J, Lamers WH, Koehler ES, Geuns JM, Alhonen L, Uimari A, et al. Arginase-1-independent polyamine production stimulates the expression of IL-4-induced alternatively activated macrophage markers while inhibiting LPS-induced expression of inflammatory genes. JOURNAL OF LEUKOCYTE BIOLOGY. 2012;91(5):685–99.
MLA
Van den Bossche, Jan, Wouter H Lamers, Eleonorre S Koehler, et al. “Arginase-1-independent Polyamine Production Stimulates the Expression of IL-4-induced Alternatively Activated Macrophage Markers While Inhibiting LPS-induced Expression of Inflammatory Genes.” JOURNAL OF LEUKOCYTE BIOLOGY 91.5 (2012): 685–699. Print.