Ghent University Academic Bibliography

Advanced

A novel putative auxin carrier family regulates intracellular auxin homeostasis in plants

Elke Barbez UGent, Martin Kubeš, Jakub Rolčík, Chloé Beziat UGent, Aleš Pěnčík, Bangjun Wang, Michel Ruiz Rosquete, Jinsheng Zhu, Petre I Dobrev and Yuree Lee, et al. (2012) NATURE. 485(7396). p.119-U155
abstract
The phytohormone auxin acts as a prominent signal, providing, by its local accumulation or depletion in selected cells, a spatial and temporal reference for changes in the developmental program(1-7). The distribution of auxin depends on both auxin metabolism (biosynthesis, conjugation and degradation)(8-10) and cellular auxin transport(11-15). We identified in silico a novel putative auxin transport facilitator family, called PIN-LIKES (PILS). Here we illustrate that PILS proteins are required for auxin-dependent regulation of plant growth by determining the cellular sensitivity to auxin. PILS proteins regulate intracellular auxin accumulation at the endoplasmic reticulum and thus auxin availability for nuclear auxin signalling. PILS activity affects the level of endogenous auxin indole-3-acetic acid (IAA), presumably via intracellular accumulation and metabolism. Our findings reveal that the transport machinery to compartmentalize auxin within the cell is of an unexpected molecular complexity and demonstrate this compartmentalization to be functionally important for a number of developmental processes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ARABIDOPSIS, EFFLUX, ABSCISIC-ACID, TOBACCO CELLS, TRANSPORT, SOLID-PHASE EXTRACTION, PROTEINS, BIOSYNTHESIS, PURIFICATION, LOCALIZATION
journal title
NATURE
Nature
volume
485
issue
7396
pages
119 - U155
Web of Science type
Article
Web of Science id
000303451900046
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
38.597 (2012)
JCR rank
1/56 (2012)
JCR quartile
1 (2012)
ISSN
0028-0836
DOI
10.1038/nature11001
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2975378
handle
http://hdl.handle.net/1854/LU-2975378
date created
2012-08-28 08:59:00
date last changed
2013-12-12 09:50:12
@article{2975378,
  abstract     = {The phytohormone auxin acts as a prominent signal, providing, by its local accumulation or depletion in selected cells, a spatial and temporal reference for changes in the developmental program(1-7). The distribution of auxin depends on both auxin metabolism (biosynthesis, conjugation and degradation)(8-10) and cellular auxin transport(11-15). We identified in silico a novel putative auxin transport facilitator family, called PIN-LIKES (PILS). Here we illustrate that PILS proteins are required for auxin-dependent regulation of plant growth by determining the cellular sensitivity to auxin. PILS proteins regulate intracellular auxin accumulation at the endoplasmic reticulum and thus auxin availability for nuclear auxin signalling. PILS activity affects the level of endogenous auxin indole-3-acetic acid (IAA), presumably via intracellular accumulation and metabolism. Our findings reveal that the transport machinery to compartmentalize auxin within the cell is of an unexpected molecular complexity and demonstrate this compartmentalization to be functionally important for a number of developmental processes.},
  author       = {Barbez, Elke and Kube\v{s}, Martin and Rol\v{c}{\'i}k, Jakub and Beziat, Chlo{\'e} and P\v{e}n\v{c}{\'i}k, Ale\v{s} and Wang, Bangjun and Rosquete, Michel Ruiz and Zhu, Jinsheng and Dobrev, Petre I and Lee, Yuree and Za\v{z}{\'i}malov{\`a}, Eva and Petr{\'a}\v{s}ek, Jan and Geisler, Markus and Friml, Jiri and Kleine-Vehn, J{\"u}rgen},
  issn         = {0028-0836},
  journal      = {NATURE},
  keyword      = {ARABIDOPSIS,EFFLUX,ABSCISIC-ACID,TOBACCO CELLS,TRANSPORT,SOLID-PHASE EXTRACTION,PROTEINS,BIOSYNTHESIS,PURIFICATION,LOCALIZATION},
  language     = {eng},
  number       = {7396},
  pages        = {119--U155},
  title        = {A novel putative auxin carrier family regulates intracellular auxin homeostasis in plants},
  url          = {http://dx.doi.org/10.1038/nature11001},
  volume       = {485},
  year         = {2012},
}

Chicago
Barbez, Elke, Martin Kubeš, Jakub Rolčík, Chloé Beziat, Aleš Pěnčík, Bangjun Wang, Michel Ruiz Rosquete, et al. 2012. “A Novel Putative Auxin Carrier Family Regulates Intracellular Auxin Homeostasis in Plants.” Nature 485 (7396): 119–U155.
APA
Barbez, E., Kubeš, M., Rolčík, J., Beziat, C., Pěnčík, A., Wang, B., Rosquete, M. R., et al. (2012). A novel putative auxin carrier family regulates intracellular auxin homeostasis in plants. NATURE, 485(7396), 119–U155.
Vancouver
1.
Barbez E, Kubeš M, Rolčík J, Beziat C, Pěnčík A, Wang B, et al. A novel putative auxin carrier family regulates intracellular auxin homeostasis in plants. NATURE. 2012;485(7396):119–U155.
MLA
Barbez, Elke, Martin Kubeš, Jakub Rolčík, et al. “A Novel Putative Auxin Carrier Family Regulates Intracellular Auxin Homeostasis in Plants.” NATURE 485.7396 (2012): 119–U155. Print.