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Role of heat-stable enterotoxins in the induction of early immune responses in piglets after infection with enterotoxigenic Escherichia coli

Michaela Loos UGent, Marisa Geens, Stijn Schauvliege UGent, Frank Gasthuys UGent, Jan van der Meulen, J Daniel Dubreuil, Bruno Goddeeris UGent, Theo Niewold and Eric Cox UGent (2012) PLOS ONE. 7(7).
abstract
Enterotoxigenic Escherichia coli (ETEC) strains that produce heat-stable (ST) and/or heat - labile (LT) enterotoxins are cause of post - weaning diarrhea in piglets. However, the relative importance of the different enterotoxins in host immune responses against ETEC infection has been poorly defined. In the present study, several isogenic mutant strains of an O149:F4ac(+), LT+ STa+ STb+ ETEC strain were constructed that lack the expression of LT in combination with one or both types of ST enterotoxins (STa and/or STb). The small intestinal segment perfusion (SISP) technique and microarray analysis were used to study host early immune responses induced by these mutant strains 4 h after infection in comparison to the wild type strain and a PBS control. Simultaneously, net fluid absorption of pig small intestinal mucosa was measured 4 h after infection, allowing us to correlate enterotoxin secretion with gene regulation. Microarray analysis showed on the one hand a non-toxin related general antibacterial response comprising genes such as PAP, MMP1 and IL8. On the other hand, results suggest a dominant role for STb in small intestinal secretion early after post-weaning infection, as well as in the induced innate immune response through differential regulation of immune mediators like interleukin 1 and interleukin 17.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CYTOKINE EXPRESSION, HUMAN MONOCYTES, DENDRITIC CELLS, GENE-EXPRESSION, LABILE ENTEROTOXIN, POSTWEANING DIARRHEA, INTESTINAL EPITHELIAL-CELLS, TOLL-LIKE-RECEPTOR, PANCREATITIS-ASSOCIATED PROTEIN, NET ABSORPTION
journal title
PLOS ONE
PLoS One
volume
7
issue
7
article_number
e41041
pages
18 pages
Web of Science type
Article
Web of Science id
000306507000054
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.73 (2012)
JCR rank
7/56 (2012)
JCR quartile
1 (2012)
ISSN
1932-6203
DOI
10.1371/journal.pone.0041041
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2972438
handle
http://hdl.handle.net/1854/LU-2972438
date created
2012-08-16 15:16:05
date last changed
2012-09-04 12:38:56
@article{2972438,
  abstract     = {Enterotoxigenic Escherichia coli (ETEC) strains that produce heat-stable (ST) and/or heat - labile (LT) enterotoxins are cause of post - weaning diarrhea in piglets. However, the relative importance of the different enterotoxins in host immune responses against ETEC infection has been poorly defined. In the present study, several isogenic mutant strains of an O149:F4ac(+), LT+ STa+ STb+ ETEC strain were constructed that lack the expression of LT in combination with one or both types of ST enterotoxins (STa and/or STb). The small intestinal segment perfusion (SISP) technique and microarray analysis were used to study host early immune responses induced by these mutant strains 4 h after infection in comparison to the wild type strain and a PBS control. Simultaneously, net fluid absorption of pig small intestinal mucosa was measured 4 h after infection, allowing us to correlate enterotoxin secretion with gene regulation. Microarray analysis showed on the one hand a non-toxin related general antibacterial response comprising genes such as PAP, MMP1 and IL8. On the other hand, results suggest a dominant role for STb in small intestinal secretion early after post-weaning infection, as well as in the induced innate immune response through differential regulation of immune mediators like interleukin 1 and interleukin 17.},
  articleno    = {e41041},
  author       = {Loos, Michaela and Geens, Marisa and Schauvliege, Stijn and Gasthuys, Frank and van der Meulen, Jan and Dubreuil, J Daniel and Goddeeris, Bruno and Niewold, Theo and Cox, Eric},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {CYTOKINE EXPRESSION,HUMAN MONOCYTES,DENDRITIC CELLS,GENE-EXPRESSION,LABILE ENTEROTOXIN,POSTWEANING DIARRHEA,INTESTINAL EPITHELIAL-CELLS,TOLL-LIKE-RECEPTOR,PANCREATITIS-ASSOCIATED PROTEIN,NET ABSORPTION},
  language     = {eng},
  number       = {7},
  pages        = {18},
  title        = {Role of heat-stable enterotoxins in the induction of early immune responses in piglets after infection with enterotoxigenic Escherichia coli},
  url          = {http://dx.doi.org/10.1371/journal.pone.0041041},
  volume       = {7},
  year         = {2012},
}

Chicago
Loos, Michaela, Marisa Geens, Stijn Schauvliege, Frank Gasthuys, Jan van der Meulen, J Daniel Dubreuil, Bruno Goddeeris, Theo Niewold, and Eric Cox. 2012. “Role of Heat-stable Enterotoxins in the Induction of Early Immune Responses in Piglets After Infection with Enterotoxigenic Escherichia Coli.” Plos One 7 (7).
APA
Loos, M., Geens, M., Schauvliege, S., Gasthuys, F., van der Meulen, J., Dubreuil, J. D., Goddeeris, B., et al. (2012). Role of heat-stable enterotoxins in the induction of early immune responses in piglets after infection with enterotoxigenic Escherichia coli. PLOS ONE, 7(7).
Vancouver
1.
Loos M, Geens M, Schauvliege S, Gasthuys F, van der Meulen J, Dubreuil JD, et al. Role of heat-stable enterotoxins in the induction of early immune responses in piglets after infection with enterotoxigenic Escherichia coli. PLOS ONE. 2012;7(7).
MLA
Loos, Michaela, Marisa Geens, Stijn Schauvliege, et al. “Role of Heat-stable Enterotoxins in the Induction of Early Immune Responses in Piglets After Infection with Enterotoxigenic Escherichia Coli.” PLOS ONE 7.7 (2012): n. pag. Print.