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Random mutagenesis MAPPIT analysis identifies binding sites for Vif and Gag in both cytidine deaminase domains of Apobec3G

Isabel Uyttendaele (UGent) , Delphine Lavens (UGent) , Dominiek Catteeuw (UGent) , Irma Lemmens (UGent) , Celia Bovijn (UGent) , Jan Tavernier (UGent) and Frank Peelman (UGent)
(2012) PLoS ONE. 7(9).
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Abstract
The mammalian two-hybrid system MAPPIT allows the detection of protein-protein interactions in intact human cells. We developed a random mutagenesis screening strategy based on MAPPIT to detect mutations that disrupt the interaction of one protein with multiple protein interactors simultanously. The strategy was used to detect residues of the human cytidine deaminase Apobec3G that are important for its homodimerization and its interaction with the HIV-1 Gag and Vif proteins. The strategy is able to identify the previously described head-to-head homodimerization interface in the N-terminal domain of Apobec3G. Our analysis further detects two new potential interaction surfaces in the N-and C-terminal domain of Apobec3G for interaction with Vif and Gag or for Apobec3G dimerization.
Keywords
INTERACTION TRAP, MAMMALIAN-CELLS, INTERACTOME NETWORK, ANTIVIRAL ACTIVITY, VIRION INFECTIVITY FACTOR, MULTIPLE SEQUENCE ALIGNMENT, HIV-1 REVERSE TRANSCRIPTION, PROTEIN-PROTEIN INTERACTIONS, ENZYME APOBEC3G, DEGRADATION

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Chicago
Uyttendaele, Isabel, Delphine Lavens, Dominiek Catteeuw, Irma Lemmens, Celia Bovijn, Jan Tavernier, and Frank Peelman. 2012. “Random Mutagenesis MAPPIT Analysis Identifies Binding Sites for Vif and Gag in Both Cytidine Deaminase Domains of Apobec3G.” PLoS ONE 7 (9).
APA
Uyttendaele, I., Lavens, D., Catteeuw, D., Lemmens, I., Bovijn, C., Tavernier, J., & Peelman, F. (2012). Random mutagenesis MAPPIT analysis identifies binding sites for Vif and Gag in both cytidine deaminase domains of Apobec3G. PLoS ONE, 7(9).
Vancouver
1.
Uyttendaele I, Lavens D, Catteeuw D, Lemmens I, Bovijn C, Tavernier J, et al. Random mutagenesis MAPPIT analysis identifies binding sites for Vif and Gag in both cytidine deaminase domains of Apobec3G. PLoS ONE. 2012;7(9).
MLA
Uyttendaele, Isabel, Delphine Lavens, Dominiek Catteeuw, et al. “Random Mutagenesis MAPPIT Analysis Identifies Binding Sites for Vif and Gag in Both Cytidine Deaminase Domains of Apobec3G.” PLoS ONE 7.9 (2012): n. pag. Print.
@article{2967356,
  abstract     = {The mammalian two-hybrid system MAPPIT allows the detection of protein-protein interactions in intact human cells. We developed a random mutagenesis screening strategy based on MAPPIT to detect mutations that disrupt the interaction of one protein with multiple protein interactors simultanously. The strategy was used to detect residues of the human cytidine deaminase Apobec3G that are important for its homodimerization and its interaction with the HIV-1 Gag and Vif proteins. The strategy is able to identify the previously described head-to-head homodimerization interface in the N-terminal domain of Apobec3G. Our analysis further detects two new potential interaction surfaces in the N-and C-terminal domain of Apobec3G for interaction with Vif and Gag or for Apobec3G dimerization.},
  articleno    = {e44143},
  author       = {Uyttendaele, Isabel and Lavens, Delphine and Catteeuw, Dominiek and Lemmens, Irma and Bovijn, Celia and Tavernier, Jan and Peelman, Frank},
  issn         = {1932-6203},
  journal      = {PLoS ONE},
  language     = {eng},
  number       = {9},
  pages        = {15},
  title        = {Random mutagenesis MAPPIT analysis identifies binding sites for Vif and Gag in both cytidine deaminase domains of Apobec3G},
  url          = {http://dx.doi.org/10.1371/journal.pone.0044143},
  volume       = {7},
  year         = {2012},
}

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