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Random mutagenesis MAPPIT analysis identifies binding sites for Vif and Gag in both cytidine deaminase domains of Apobec3G

Isabel Uyttendaele UGent, Delphine Lavens UGent, Dominiek Catteeuw UGent, Irma Lemmens UGent, Celia Bovijn UGent, Jan Tavernier UGent and Frank Peelman UGent (2012) PLoS ONE. 7(9).
abstract
The mammalian two-hybrid system MAPPIT allows the detection of protein-protein interactions in intact human cells. We developed a random mutagenesis screening strategy based on MAPPIT to detect mutations that disrupt the interaction of one protein with multiple protein interactors simultanously. The strategy was used to detect residues of the human cytidine deaminase Apobec3G that are important for its homodimerization and its interaction with the HIV-1 Gag and Vif proteins. The strategy is able to identify the previously described head-to-head homodimerization interface in the N-terminal domain of Apobec3G. Our analysis further detects two new potential interaction surfaces in the N-and C-terminal domain of Apobec3G for interaction with Vif and Gag or for Apobec3G dimerization.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
INTERACTION TRAP, MAMMALIAN-CELLS, INTERACTOME NETWORK, ANTIVIRAL ACTIVITY, VIRION INFECTIVITY FACTOR, MULTIPLE SEQUENCE ALIGNMENT, HIV-1 REVERSE TRANSCRIPTION, PROTEIN-PROTEIN INTERACTIONS, ENZYME APOBEC3G, DEGRADATION
journal title
PLoS ONE
PLoS ONE
volume
7
issue
9
article_number
e44143
pages
15 pages
Web of Science type
Article
Web of Science id
000308748400011
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.73 (2012)
JCR rank
7/56 (2012)
JCR quartile
1 (2012)
ISSN
1932-6203
DOI
10.1371/journal.pone.0044143
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2967356
handle
http://hdl.handle.net/1854/LU-2967356
date created
2012-08-03 11:35:10
date last changed
2012-11-08 10:16:48
@article{2967356,
  abstract     = {The mammalian two-hybrid system MAPPIT allows the detection of protein-protein interactions in intact human cells. We developed a random mutagenesis screening strategy based on MAPPIT to detect mutations that disrupt the interaction of one protein with multiple protein interactors simultanously. The strategy was used to detect residues of the human cytidine deaminase Apobec3G that are important for its homodimerization and its interaction with the HIV-1 Gag and Vif proteins. The strategy is able to identify the previously described head-to-head homodimerization interface in the N-terminal domain of Apobec3G. Our analysis further detects two new potential interaction surfaces in the N-and C-terminal domain of Apobec3G for interaction with Vif and Gag or for Apobec3G dimerization.},
  articleno    = {e44143},
  author       = {Uyttendaele, Isabel and Lavens, Delphine and Catteeuw, Dominiek and Lemmens, Irma and Bovijn, Celia and Tavernier, Jan and Peelman, Frank},
  issn         = {1932-6203},
  journal      = {PLoS ONE},
  keyword      = {INTERACTION TRAP,MAMMALIAN-CELLS,INTERACTOME NETWORK,ANTIVIRAL ACTIVITY,VIRION INFECTIVITY FACTOR,MULTIPLE SEQUENCE ALIGNMENT,HIV-1 REVERSE TRANSCRIPTION,PROTEIN-PROTEIN INTERACTIONS,ENZYME APOBEC3G,DEGRADATION},
  language     = {eng},
  number       = {9},
  pages        = {15},
  title        = {Random mutagenesis MAPPIT analysis identifies binding sites for Vif and Gag in both cytidine deaminase domains of Apobec3G},
  url          = {http://dx.doi.org/10.1371/journal.pone.0044143},
  volume       = {7},
  year         = {2012},
}

Chicago
Uyttendaele, Isabel, Delphine Lavens, Dominiek Catteeuw, Irma Lemmens, Celia Bovijn, Jan Tavernier, and Frank Peelman. 2012. “Random Mutagenesis MAPPIT Analysis Identifies Binding Sites for Vif and Gag in Both Cytidine Deaminase Domains of Apobec3G.” PLoS ONE 7 (9).
APA
Uyttendaele, I., Lavens, D., Catteeuw, D., Lemmens, I., Bovijn, C., Tavernier, J., & Peelman, F. (2012). Random mutagenesis MAPPIT analysis identifies binding sites for Vif and Gag in both cytidine deaminase domains of Apobec3G. PLoS ONE, 7(9).
Vancouver
1.
Uyttendaele I, Lavens D, Catteeuw D, Lemmens I, Bovijn C, Tavernier J, et al. Random mutagenesis MAPPIT analysis identifies binding sites for Vif and Gag in both cytidine deaminase domains of Apobec3G. PLoS ONE. 2012;7(9).
MLA
Uyttendaele, Isabel, Delphine Lavens, Dominiek Catteeuw, et al. “Random Mutagenesis MAPPIT Analysis Identifies Binding Sites for Vif and Gag in Both Cytidine Deaminase Domains of Apobec3G.” PLoS ONE 7.9 (2012): n. pag. Print.