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Elution behavior of short dsDNA strands in silicon micropillar array columns in ion pair reversed-phase chromatography mode

Lei Zhang, Bivragh Majeed, Frederic Lynen UGent, Chris Van Hoof and Wim De Malsche (2012) ELECTROPHORESIS. 33(21). p.3205-3212
abstract
In the present paper, dsDNA separation has been studied in a silicon micro-pillar array column using ion-pair reversed-phase HPLC (IP-RP-HPLC). The deep-etched (32.0μm) silicon micro-pillar array was fabricated by advanced deep-UV lithography and by a dedicated Bosch etch process and then sealed by anodic bonding to a Pyrex glass. The pillar surface was subsequently conditioned hydrophobic. Working in isocratic mode under non-retained conditions, van Deemter curves of dsDNA and coumarin were established to assess the performance of the micro-pillar array column, resulting in plate heights of only a few μm. Working in gradient mode, separations of dsDNA fragments were evaluated. The relevant gradient operation parameters were studied to understand their influence on dsDNA separations. The correlation between DNA length and retention was measured and theoretically described in a length range of 50-500bp, promising for the determination of DNA of an unknown length. Finally, a separation example demonstrated the excellent separation power of on-chip IP-RP chromatography by achieving a large operation range of DNA length (10-300bp) with a 5bp difference among 11 dsDNA fragments.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Lab on a chip, silicon micro-pillar array column, DNA chromatography, Ion-pair RP-HPLC, PERFORMANCE LIQUID-CHROMATOGRAPHY, CAPILLARY-ELECTROPHORESIS, MICROFLUIDIC CHIP, GRADIENT-ELUTION, DNA FRAGMENTS, SEPARATION, FABRICATION, MICROCHIP
journal title
ELECTROPHORESIS
Electrophoresis
volume
33
issue
21
pages
3205 - 3212
Web of Science type
Article
Web of Science id
000310476600012
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.261 (2012)
JCR rank
14/74 (2012)
JCR quartile
1 (2012)
ISSN
0173-0835
DOI
10.1002/elps.201200226
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2967148
handle
http://hdl.handle.net/1854/LU-2967148
date created
2012-08-02 16:46:13
date last changed
2013-07-15 13:21:00
@article{2967148,
  abstract     = {In the present paper, dsDNA separation has been studied in a silicon micro-pillar array column using ion-pair reversed-phase HPLC (IP-RP-HPLC). The deep-etched (32.0\ensuremath{\mu}m) silicon micro-pillar array was fabricated by advanced deep-UV lithography and by a dedicated Bosch etch process and then sealed by anodic bonding to a Pyrex glass. The pillar surface was subsequently conditioned hydrophobic. Working in isocratic mode under non-retained conditions, van Deemter curves of dsDNA and coumarin were established to assess the performance of the micro-pillar array column, resulting in plate heights of only a few \ensuremath{\mu}m. Working in gradient mode, separations of dsDNA fragments were evaluated. The relevant gradient operation parameters were studied to understand their influence on dsDNA separations. The correlation between DNA length and retention was measured and theoretically described in a length range of 50-500bp, promising for the determination of DNA of an unknown length. Finally, a separation example demonstrated the excellent separation power of on-chip IP-RP chromatography by achieving a large operation range of DNA length (10-300bp) with a 5bp difference among 11 dsDNA fragments.},
  author       = {Zhang, Lei and Majeed, Bivragh  and Lynen, Frederic and Van Hoof, Chris  and De Malsche, Wim },
  issn         = {0173-0835},
  journal      = {ELECTROPHORESIS},
  keyword      = {Lab on a chip,silicon micro-pillar array column,DNA chromatography,Ion-pair RP-HPLC,PERFORMANCE LIQUID-CHROMATOGRAPHY,CAPILLARY-ELECTROPHORESIS,MICROFLUIDIC CHIP,GRADIENT-ELUTION,DNA FRAGMENTS,SEPARATION,FABRICATION,MICROCHIP},
  language     = {eng},
  number       = {21},
  pages        = {3205--3212},
  title        = {Elution behavior of short dsDNA strands in silicon micropillar array columns in ion pair reversed-phase chromatography mode},
  url          = {http://dx.doi.org/10.1002/elps.201200226},
  volume       = {33},
  year         = {2012},
}

Chicago
Zhang, Lei, Bivragh Majeed, Frederic Lynen, Chris Van Hoof, and Wim De Malsche. 2012. “Elution Behavior of Short dsDNA Strands in Silicon Micropillar Array Columns in Ion Pair Reversed-phase Chromatography Mode.” Electrophoresis 33 (21): 3205–3212.
APA
Zhang, Lei, Majeed, B., Lynen, F., Van Hoof, C., & De Malsche, W. (2012). Elution behavior of short dsDNA strands in silicon micropillar array columns in ion pair reversed-phase chromatography mode. ELECTROPHORESIS, 33(21), 3205–3212.
Vancouver
1.
Zhang L, Majeed B, Lynen F, Van Hoof C, De Malsche W. Elution behavior of short dsDNA strands in silicon micropillar array columns in ion pair reversed-phase chromatography mode. ELECTROPHORESIS. 2012;33(21):3205–12.
MLA
Zhang, Lei, Bivragh Majeed, Frederic Lynen, et al. “Elution Behavior of Short dsDNA Strands in Silicon Micropillar Array Columns in Ion Pair Reversed-phase Chromatography Mode.” ELECTROPHORESIS 33.21 (2012): 3205–3212. Print.