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De novo transcriptome assembly and SNP discovery in the wing polymorphic salt marsh beetle Pogonus chalceus (Coleoptera, Carabidae)

Steven Van Belleghem UGent, Dick Roelofs, Jeroen Van Houdt and Frederik Hendrickx UGent (2012) PLOS ONE. 7(8).
abstract
Background : The salt marsh beetle Pogonus chalceus represents a unique opportunity to understand and study the origin and evolution of dispersal polymorphisms as remarkable inter-population divergence in dispersal related traits (e.g. wing development, body size and metabolism) has been shown to persist in face of strong homogenizing gene flow. Sequencing and assembling the transcriptome of P. chalceus is a first step in developing large scale genetic information that will allow us to further study the recurrent phenotypic evolution in dispersal traits in these natural populations. Methodology/Results : We used the Illumina HiSeq2000 to sequence 37 Gbases of the transcriptome and performed de novo transcriptome assembly with the Trinity short read assembler. This resulted in 65,766 contigs, clustering into 39,393 unique transcripts (unigenes). A subset of 12,987 show similarity (BLAST) to known proteins in the NCBI database and 7,589 are assigned Gene Ontology (GO). Using homology searches we identified all reported genes involved in wing development, juvenile- and ecdysteroid hormone pathways in Tribolium castaneum. About half (56.7%) of the unique assembled genes are shared among three life stages (third-instar larva, pupa, and imago). We identified 38,141 single nucleotide polymorphisms (SNPs) in these unigenes. Of these SNPs, 26,823 (70.3%) were found in a predicted open reading frame (ORF) and 6,998 (18.3%) were nonsynonymous. Conclusions : The assembled transcriptome and SNP data are essential genomic resources for further study of the developmental pathways, genetic mechanisms and metabolic consequences of adaptive divergence in dispersal power in natural populations.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
PLOS ONE
PLoS One
volume
7
issue
8
article_number
e42605
pages
13 pages
Web of Science type
Article
Web of Science id
000307212800101
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.73 (2012)
JCR rank
7/56 (2012)
JCR quartile
1 (2012)
ISSN
1932-6203
DOI
10.1371/journal.pone.0042605
project
HPC-UGent: the central High Performance Computing infrastructure of Ghent University
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2966955
handle
http://hdl.handle.net/1854/LU-2966955
date created
2012-08-02 13:54:02
date last changed
2013-09-17 10:49:46
@article{2966955,
  abstract     = {Background : The salt marsh beetle Pogonus chalceus represents a unique opportunity to understand and study the origin and evolution of dispersal polymorphisms as remarkable inter-population divergence in dispersal related traits (e.g. wing development, body size and metabolism) has been shown to persist in face of strong homogenizing gene flow. Sequencing and assembling the transcriptome of P. chalceus is a first step in developing large scale genetic information that will allow us to further study the recurrent phenotypic evolution in dispersal traits in these natural populations.
Methodology/Results : We used the Illumina HiSeq2000 to sequence 37 Gbases of the transcriptome and performed de novo transcriptome assembly with the Trinity short read assembler. This resulted in 65,766 contigs, clustering into 39,393 unique transcripts (unigenes). A subset of 12,987 show similarity (BLAST) to known proteins in the NCBI database and 7,589 are assigned Gene Ontology (GO). Using homology searches we identified all reported genes involved in wing development, juvenile- and ecdysteroid hormone pathways in Tribolium castaneum. About half (56.7\%) of the unique assembled genes are shared among three life stages (third-instar larva, pupa, and imago). We identified 38,141 single nucleotide polymorphisms (SNPs) in these unigenes. Of these SNPs, 26,823 (70.3\%) were found in a predicted open reading frame (ORF) and 6,998 (18.3\%) were nonsynonymous.
Conclusions : The assembled transcriptome and SNP data are essential genomic resources for further study of the developmental pathways, genetic mechanisms and metabolic consequences of adaptive divergence in dispersal power in natural populations.},
  articleno    = {e42605},
  author       = {Van Belleghem, Steven and Roelofs, Dick and Van Houdt, Jeroen and Hendrickx, Frederik},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  language     = {eng},
  number       = {8},
  pages        = {13},
  title        = {De novo transcriptome assembly and SNP discovery in the wing polymorphic salt marsh beetle Pogonus chalceus (Coleoptera, Carabidae)},
  url          = {http://dx.doi.org/10.1371/journal.pone.0042605},
  volume       = {7},
  year         = {2012},
}

Chicago
Van Belleghem, Steven, Dick Roelofs, Jeroen Van Houdt, and Frederik Hendrickx. 2012. “De Novo Transcriptome Assembly and SNP Discovery in the Wing Polymorphic Salt Marsh Beetle Pogonus Chalceus (Coleoptera, Carabidae).” Plos One 7 (8).
APA
Van Belleghem, S., Roelofs, D., Van Houdt, J., & Hendrickx, F. (2012). De novo transcriptome assembly and SNP discovery in the wing polymorphic salt marsh beetle Pogonus chalceus (Coleoptera, Carabidae). PLOS ONE, 7(8).
Vancouver
1.
Van Belleghem S, Roelofs D, Van Houdt J, Hendrickx F. De novo transcriptome assembly and SNP discovery in the wing polymorphic salt marsh beetle Pogonus chalceus (Coleoptera, Carabidae). PLOS ONE. 2012;7(8).
MLA
Van Belleghem, Steven, Dick Roelofs, Jeroen Van Houdt, et al. “De Novo Transcriptome Assembly and SNP Discovery in the Wing Polymorphic Salt Marsh Beetle Pogonus Chalceus (Coleoptera, Carabidae).” PLOS ONE 7.8 (2012): n. pag. Print.