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Delivery of dsRNA for RNAi in insects: an overview and future directions

Na Yu (UGent) , Olivier Christiaens (UGent) , Jisheng Liu (UGent) , Kaat Cappelle, Silvia Caccia (UGent) , Hanneke Huvenne (UGent) and Guy Smagghe (UGent)
(2013) INSECT SCIENCE. 20(1). p.4-14
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Abstract
RNA interference (RNAi) refers to the process of exogenous double-stranded RNA (dsRNA) silencing the complementary endogenous messenger RNA. RNAi has been widely used in entomological research for functional genomics in a variety of insects and its potential for RNAi-based pest control has been increasingly emphasized mainly because of its high specificity. This review focuses on the approaches of introducing dsRNA into insect cells or insect bodies to induce effective RNAi. The three most common delivery methods, namely, microinjection, ingestion, and soaking, are illustrated in details and their advantages and limitations are summarized for purpose of feasible RNAi research. In this review, we also briefly introduce the two possible dsRNA uptake machineries, other dsRNA delivery methods and the history of RNAi in entomology. Factors that influence the specificity and efficiency of RNAi such as transfection reagents, selection of dsRNA region, length, and stability of dsRNA in RNAi research are discussed for further studies.
Keywords
ingestion, dsRNA uptake, microinjection, pest control, RNA interference, soaking, DOUBLE-STRANDED-RNA, RED FLOUR BEETLE, TRANSMEMBRANE PROTEIN SID-1, CRICKET GRYLLUS-BIMACULATUS, HIGH-THROUGHPUT RNAI, DROSOPHILA S2 CELLS, TRIBOLIUM-CASTANEUM, CAENORHABDITIS-ELEGANS, GENETIC INTERFERENCE, FUNCTIONAL GENOMICS

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Chicago
Yu, Na, Olivier Christiaens, Jisheng Liu, Kaat Cappelle, Silvia Caccia, Hanneke Huvenne, and Guy Smagghe. 2013. “Delivery of dsRNA for RNAi in Insects: An Overview and Future Directions.” Insect Science 20 (1): 4–14.
APA
Yu, N., Christiaens, O., Liu, J., Cappelle, K., Caccia, S., Huvenne, H., & Smagghe, G. (2013). Delivery of dsRNA for RNAi in insects: an overview and future directions. INSECT SCIENCE, 20(1), 4–14.
Vancouver
1.
Yu N, Christiaens O, Liu J, Cappelle K, Caccia S, Huvenne H, et al. Delivery of dsRNA for RNAi in insects: an overview and future directions. INSECT SCIENCE. 2013;20(1):4–14.
MLA
Yu, Na, Olivier Christiaens, Jisheng Liu, et al. “Delivery of dsRNA for RNAi in Insects: An Overview and Future Directions.” INSECT SCIENCE 20.1 (2013): 4–14. Print.
@article{2965817,
  abstract     = {RNA interference (RNAi) refers to the process of exogenous double-stranded RNA (dsRNA) silencing the complementary endogenous messenger RNA. RNAi has been widely used in entomological research for functional genomics in a variety of insects and its potential for RNAi-based pest control has been increasingly emphasized mainly because of its high specificity. This review focuses on the approaches of introducing dsRNA into insect cells or insect bodies to induce effective RNAi. The three most common delivery methods, namely, microinjection, ingestion, and soaking, are illustrated in details and their advantages and limitations are summarized for purpose of feasible RNAi research. In this review, we also briefly introduce the two possible dsRNA uptake machineries, other dsRNA delivery methods and the history of RNAi in entomology. Factors that influence the specificity and efficiency of RNAi such as transfection reagents, selection of dsRNA region, length, and stability of dsRNA in RNAi research are discussed for further studies.},
  author       = {Yu, Na and Christiaens, Olivier and Liu, Jisheng and Cappelle, Kaat and Caccia, Silvia and Huvenne, Hanneke and Smagghe, Guy},
  issn         = {1672-9609},
  journal      = {INSECT SCIENCE},
  language     = {eng},
  number       = {1},
  pages        = {4--14},
  title        = {Delivery of dsRNA for RNAi in insects: an overview and future directions},
  url          = {http://dx.doi.org/10.1111/j.1744-7917.2012.01534.x},
  volume       = {20},
  year         = {2013},
}

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