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Highly dynamic exon shuffling in candidate pathogen receptors... : what if brown algae were capable of adaptive immunity?

(2012) MOLECULAR BIOLOGY AND EVOLUTION. 29(4). p.1263-1276
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Abstract
Pathogen recognition is the first step of immune reactions. In animals and plants, direct or indirect pathogen recognition is often mediated by a wealth of fast-evolving receptors, many of which contain ligand-binding and signal transduction domains, such as leucine-rich or tetratricopeptide repeat (LRR/TPR) and NB-ARC domains, respectively. In order to identify candidates potentially involved in algal defense, we mined the genome of the brown alga Ectocarpus siliculosus for homologues of these genes and assessed the evolutionary pressures acting upon them. We thus annotated all Ectocarpus LRR-containing genes, in particular an original group of LRR-containing GTPases of the ROCO family, and 24 NB-ARC-TPR proteins. They exhibit high birth and death rates, while a diversifying selection is acting on their LRR (respectively TPR) domain, probably affecting the ligand-binding specificities. Remarkably, each repeat is encoded by an exon, and the intense exon shuffling underpins the variability of LRR and TPR domains. We conclude that the Ectocarpus ROCO and NB-ARC-TPR families are excellent candidates for being involved in recognition/transduction events linked to immunity. We further hypothesize that brown algae may generate their immune repertoire via controlled somatic recombination, so far only known from the vertebrate adaptive immune systems.
Keywords
PHYLOGENETIC ANALYSIS, NONSYNONYMOUS SUBSTITUTION, MAXIMUM-LIKELIHOOD, RESISTANCE GENES, PROTEIN, PLANT, EVOLUTION, REPEAT, RECOGNITION, adaptive immunity, VARIABLE LYMPHOCYTE RECEPTORS, innate immunity, resistance gene analogue, exon shuffling, Ectocarpus, brown alga

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MLA
Zambounis, Antonios et al. “Highly Dynamic Exon Shuffling in Candidate Pathogen Receptors... : What If Brown Algae Were Capable of Adaptive Immunity?” MOLECULAR BIOLOGY AND EVOLUTION 29.4 (2012): 1263–1276. Print.
APA
Zambounis, A., Elias, M., Sterck, L., Maumus, F., & Gachon, C. M. (2012). Highly dynamic exon shuffling in candidate pathogen receptors... : what if brown algae were capable of adaptive immunity? MOLECULAR BIOLOGY AND EVOLUTION, 29(4), 1263–1276.
Chicago author-date
Zambounis, Antonios, Marek Elias, Lieven Sterck, Florian Maumus, and Claire MM Gachon. 2012. “Highly Dynamic Exon Shuffling in Candidate Pathogen Receptors... : What If Brown Algae Were Capable of Adaptive Immunity?” Molecular Biology and Evolution 29 (4): 1263–1276.
Chicago author-date (all authors)
Zambounis, Antonios, Marek Elias, Lieven Sterck, Florian Maumus, and Claire MM Gachon. 2012. “Highly Dynamic Exon Shuffling in Candidate Pathogen Receptors... : What If Brown Algae Were Capable of Adaptive Immunity?” Molecular Biology and Evolution 29 (4): 1263–1276.
Vancouver
1.
Zambounis A, Elias M, Sterck L, Maumus F, Gachon CM. Highly dynamic exon shuffling in candidate pathogen receptors... : what if brown algae were capable of adaptive immunity? MOLECULAR BIOLOGY AND EVOLUTION. 2012;29(4):1263–76.
IEEE
[1]
A. Zambounis, M. Elias, L. Sterck, F. Maumus, and C. M. Gachon, “Highly dynamic exon shuffling in candidate pathogen receptors... : what if brown algae were capable of adaptive immunity?,” MOLECULAR BIOLOGY AND EVOLUTION, vol. 29, no. 4, pp. 1263–1276, 2012.
@article{2965716,
  abstract     = {Pathogen recognition is the first step of immune reactions. In animals and plants, direct or indirect pathogen recognition is often mediated by a wealth of fast-evolving receptors, many of which contain ligand-binding and signal transduction domains, such as leucine-rich or tetratricopeptide repeat (LRR/TPR) and NB-ARC domains, respectively. In order to identify candidates potentially involved in algal defense, we mined the genome of the brown alga Ectocarpus siliculosus for homologues of these genes and assessed the evolutionary pressures acting upon them. We thus annotated all Ectocarpus LRR-containing genes, in particular an original group of LRR-containing GTPases of the ROCO family, and 24 NB-ARC-TPR proteins. They exhibit high birth and death rates, while a diversifying selection is acting on their LRR (respectively TPR) domain, probably affecting the ligand-binding specificities. Remarkably, each repeat is encoded by an exon, and the intense exon shuffling underpins the variability of LRR and TPR domains. We conclude that the Ectocarpus ROCO and NB-ARC-TPR families are excellent candidates for being involved in recognition/transduction events linked to immunity. We further hypothesize that brown algae may generate their immune repertoire via controlled somatic recombination, so far only known from the vertebrate adaptive immune systems.},
  author       = {Zambounis, Antonios and Elias, Marek and Sterck, Lieven and Maumus, Florian and Gachon, Claire MM},
  issn         = {0737-4038},
  journal      = {MOLECULAR BIOLOGY AND EVOLUTION},
  keywords     = {PHYLOGENETIC ANALYSIS,NONSYNONYMOUS SUBSTITUTION,MAXIMUM-LIKELIHOOD,RESISTANCE GENES,PROTEIN,PLANT,EVOLUTION,REPEAT,RECOGNITION,adaptive immunity,VARIABLE LYMPHOCYTE RECEPTORS,innate immunity,resistance gene analogue,exon shuffling,Ectocarpus,brown alga},
  language     = {eng},
  number       = {4},
  pages        = {1263--1276},
  title        = {Highly dynamic exon shuffling in candidate pathogen receptors... : what if brown algae were capable of adaptive immunity?},
  url          = {http://dx.doi.org/10.1093/molbev/msr296},
  volume       = {29},
  year         = {2012},
}

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