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Characterization of a RABE (Ras gene from rat brain E) GTPase expressed during morphogenesis in the unicellular green alga Micrasterias denticulata (Zygnematophyceae, Streptophyta)

Katrijn Vannerum UGent, Riet De Rycke UGent, Jacob Pollier UGent, Alain Goossens UGent, Dirk Inzé UGent and Wim Vyverman UGent (2012) JOURNAL OF PHYCOLOGY. 48(3). p.682-692
abstract
Rab GTPases are central regulators of cell shape in land plants by coordinating vesicle trafficking during morphogenesis. To date, relatively little is known about the role of these ubiquitous signaling proteins during cell growth in microalgae, in particular in the related charophyte algae. This article identifies the first charophyte Rab GTPase, MdRABE1, in Micrasterias denticulata Breb., a convenient model organism for studying morphogenesis. Its expression correlated with the onset of morphogenesis, and structural analysis indicated that it belongs to the RABE (Ras gene from rat brain E) subclass. Confocal fluorescence and immunoelectron microscopy (IEM) of transiently GFP-MdRABE1 overexpressing interphase cells demonstrated that the GFP-MdRABE1 protein was localized to the endoplasmic reticulum, dictyosomes, exocytotic vesicles, the cell margin, the membranes of cell organelles, and in the isthmus zone around the nucleus. Although overexpression phenotyping of both N- and C-terminal green fluorescent protein (GFP) fusions failed to indicate additional functional evidence of the MdRABE1 protein due to mortality of those transgenic cells, its expression profile, bioinformatics, and intracellular localization suggest a role in vesicle trafficking during morphogenesis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PLASMA-MEMBRANE, CELL-GROWTH, BINDING PROTEINS, POLARIZED MEMBRANE-TRANSPORT, ACTIN-FILAMENT POLYMERIZATION, FAMILY, SOFTWARE, SYSTEM, SPECTRIN, Rab GTPase, overexpression, morphogenesis, Micrasterias denticulata, localization, LOCALIZATION, gene expression, green algae
journal title
JOURNAL OF PHYCOLOGY
J. Phycol.
volume
48
issue
3
pages
682 - 692
Web of Science type
Article
Web of Science id
000304810200021
JCR category
MARINE & FRESHWATER BIOLOGY
JCR impact factor
2.239 (2012)
JCR rank
28/99 (2012)
JCR quartile
2 (2012)
ISSN
0022-3646
DOI
10.1111/j.1529-8817.2012.01170.x
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2965659
handle
http://hdl.handle.net/1854/LU-2965659
date created
2012-07-30 15:07:05
date last changed
2014-05-26 10:04:18
@article{2965659,
  abstract     = {Rab GTPases are central regulators of cell shape in land plants by coordinating vesicle trafficking during morphogenesis. To date, relatively little is known about the role of these ubiquitous signaling proteins during cell growth in microalgae, in particular in the related charophyte algae. This article identifies the first charophyte Rab GTPase, MdRABE1, in Micrasterias denticulata Breb., a convenient model organism for studying morphogenesis. Its expression correlated with the onset of morphogenesis, and structural analysis indicated that it belongs to the RABE (Ras gene from rat brain E) subclass. Confocal fluorescence and immunoelectron microscopy (IEM) of transiently GFP-MdRABE1 overexpressing interphase cells demonstrated that the GFP-MdRABE1 protein was localized to the endoplasmic reticulum, dictyosomes, exocytotic vesicles, the cell margin, the membranes of cell organelles, and in the isthmus zone around the nucleus. Although overexpression phenotyping of both N- and C-terminal green fluorescent protein (GFP) fusions failed to indicate additional functional evidence of the MdRABE1 protein due to mortality of those transgenic cells, its expression profile, bioinformatics, and intracellular localization suggest a role in vesicle trafficking during morphogenesis.},
  author       = {Vannerum, Katrijn and De Rycke, Riet and Pollier, Jacob and Goossens, Alain and Inz{\'e}, Dirk and Vyverman, Wim},
  issn         = {0022-3646},
  journal      = {JOURNAL OF PHYCOLOGY},
  keyword      = {PLASMA-MEMBRANE,CELL-GROWTH,BINDING PROTEINS,POLARIZED MEMBRANE-TRANSPORT,ACTIN-FILAMENT POLYMERIZATION,FAMILY,SOFTWARE,SYSTEM,SPECTRIN,Rab GTPase,overexpression,morphogenesis,Micrasterias denticulata,localization,LOCALIZATION,gene expression,green algae},
  language     = {eng},
  number       = {3},
  pages        = {682--692},
  title        = {Characterization of a RABE (Ras gene from rat brain E) GTPase expressed during morphogenesis in the unicellular green alga Micrasterias denticulata (Zygnematophyceae, Streptophyta)},
  url          = {http://dx.doi.org/10.1111/j.1529-8817.2012.01170.x},
  volume       = {48},
  year         = {2012},
}

Chicago
Vannerum, Katrijn, Riet De Rycke, Jacob Pollier, Alain Goossens, Dirk Inzé, and Wim Vyverman. 2012. “Characterization of a RABE (Ras Gene from Rat Brain E) GTPase Expressed During Morphogenesis in the Unicellular Green Alga Micrasterias Denticulata (Zygnematophyceae, Streptophyta).” Journal of Phycology 48 (3): 682–692.
APA
Vannerum, K., De Rycke, R., Pollier, J., Goossens, A., Inzé, D., & Vyverman, W. (2012). Characterization of a RABE (Ras gene from rat brain E) GTPase expressed during morphogenesis in the unicellular green alga Micrasterias denticulata (Zygnematophyceae, Streptophyta). JOURNAL OF PHYCOLOGY, 48(3), 682–692.
Vancouver
1.
Vannerum K, De Rycke R, Pollier J, Goossens A, Inzé D, Vyverman W. Characterization of a RABE (Ras gene from rat brain E) GTPase expressed during morphogenesis in the unicellular green alga Micrasterias denticulata (Zygnematophyceae, Streptophyta). JOURNAL OF PHYCOLOGY. 2012;48(3):682–92.
MLA
Vannerum, Katrijn, Riet De Rycke, Jacob Pollier, et al. “Characterization of a RABE (Ras Gene from Rat Brain E) GTPase Expressed During Morphogenesis in the Unicellular Green Alga Micrasterias Denticulata (Zygnematophyceae, Streptophyta).” JOURNAL OF PHYCOLOGY 48.3 (2012): 682–692. Print.