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Characterization of a RABE (Ras gene from rat brain E) GTPase expressed during morphogenesis in the unicellular green alga Micrasterias denticulata (Zygnematophyceae, Streptophyta)

Katrijn Vannerum (UGent) , Riet De Rycke (UGent) , Jacob Pollier (UGent) , Alain Goossens (UGent) , Dirk Inzé (UGent) and Wim Vyverman (UGent)
(2012) JOURNAL OF PHYCOLOGY. 48(3). p.682-692
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Rab GTPases are central regulators of cell shape in land plants by coordinating vesicle trafficking during morphogenesis. To date, relatively little is known about the role of these ubiquitous signaling proteins during cell growth in microalgae, in particular in the related charophyte algae. This article identifies the first charophyte Rab GTPase, MdRABE1, in Micrasterias denticulata Breb., a convenient model organism for studying morphogenesis. Its expression correlated with the onset of morphogenesis, and structural analysis indicated that it belongs to the RABE (Ras gene from rat brain E) subclass. Confocal fluorescence and immunoelectron microscopy (IEM) of transiently GFP-MdRABE1 overexpressing interphase cells demonstrated that the GFP-MdRABE1 protein was localized to the endoplasmic reticulum, dictyosomes, exocytotic vesicles, the cell margin, the membranes of cell organelles, and in the isthmus zone around the nucleus. Although overexpression phenotyping of both N- and C-terminal green fluorescent protein (GFP) fusions failed to indicate additional functional evidence of the MdRABE1 protein due to mortality of those transgenic cells, its expression profile, bioinformatics, and intracellular localization suggest a role in vesicle trafficking during morphogenesis.
Keywords
PLASMA-MEMBRANE, CELL-GROWTH, BINDING PROTEINS, POLARIZED MEMBRANE-TRANSPORT, ACTIN-FILAMENT POLYMERIZATION, FAMILY, SOFTWARE, SYSTEM, SPECTRIN, Rab GTPase, overexpression, morphogenesis, Micrasterias denticulata, localization, LOCALIZATION, gene expression, green algae

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Citation

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Chicago
Vannerum, Katrijn, Riet De Rycke, Jacob Pollier, Alain Goossens, Dirk Inzé, and Wim Vyverman. 2012. “Characterization of a RABE (Ras Gene from Rat Brain E) GTPase Expressed During Morphogenesis in the Unicellular Green Alga Micrasterias Denticulata (Zygnematophyceae, Streptophyta).” Journal of Phycology 48 (3): 682–692.
APA
Vannerum, K., De Rycke, R., Pollier, J., Goossens, A., Inzé, D., & Vyverman, W. (2012). Characterization of a RABE (Ras gene from rat brain E) GTPase expressed during morphogenesis in the unicellular green alga Micrasterias denticulata (Zygnematophyceae, Streptophyta). JOURNAL OF PHYCOLOGY, 48(3), 682–692.
Vancouver
1.
Vannerum K, De Rycke R, Pollier J, Goossens A, Inzé D, Vyverman W. Characterization of a RABE (Ras gene from rat brain E) GTPase expressed during morphogenesis in the unicellular green alga Micrasterias denticulata (Zygnematophyceae, Streptophyta). JOURNAL OF PHYCOLOGY. 2012;48(3):682–92.
MLA
Vannerum, Katrijn, Riet De Rycke, Jacob Pollier, et al. “Characterization of a RABE (Ras Gene from Rat Brain E) GTPase Expressed During Morphogenesis in the Unicellular Green Alga Micrasterias Denticulata (Zygnematophyceae, Streptophyta).” JOURNAL OF PHYCOLOGY 48.3 (2012): 682–692. Print.
@article{2965659,
  abstract     = {Rab GTPases are central regulators of cell shape in land plants by coordinating vesicle trafficking during morphogenesis. To date, relatively little is known about the role of these ubiquitous signaling proteins during cell growth in microalgae, in particular in the related charophyte algae. This article identifies the first charophyte Rab GTPase, MdRABE1, in Micrasterias denticulata Breb., a convenient model organism for studying morphogenesis. Its expression correlated with the onset of morphogenesis, and structural analysis indicated that it belongs to the RABE (Ras gene from rat brain E) subclass. Confocal fluorescence and immunoelectron microscopy (IEM) of transiently GFP-MdRABE1 overexpressing interphase cells demonstrated that the GFP-MdRABE1 protein was localized to the endoplasmic reticulum, dictyosomes, exocytotic vesicles, the cell margin, the membranes of cell organelles, and in the isthmus zone around the nucleus. Although overexpression phenotyping of both N- and C-terminal green fluorescent protein (GFP) fusions failed to indicate additional functional evidence of the MdRABE1 protein due to mortality of those transgenic cells, its expression profile, bioinformatics, and intracellular localization suggest a role in vesicle trafficking during morphogenesis.},
  author       = {Vannerum, Katrijn and De Rycke, Riet and Pollier, Jacob and Goossens, Alain and Inz{\'e}, Dirk and Vyverman, Wim},
  issn         = {0022-3646},
  journal      = {JOURNAL OF PHYCOLOGY},
  keyword      = {PLASMA-MEMBRANE,CELL-GROWTH,BINDING PROTEINS,POLARIZED MEMBRANE-TRANSPORT,ACTIN-FILAMENT POLYMERIZATION,FAMILY,SOFTWARE,SYSTEM,SPECTRIN,Rab GTPase,overexpression,morphogenesis,Micrasterias denticulata,localization,LOCALIZATION,gene expression,green algae},
  language     = {eng},
  number       = {3},
  pages        = {682--692},
  title        = {Characterization of a RABE (Ras gene from rat brain E) GTPase expressed during morphogenesis in the unicellular green alga Micrasterias denticulata (Zygnematophyceae, Streptophyta)},
  url          = {http://dx.doi.org/10.1111/j.1529-8817.2012.01170.x},
  volume       = {48},
  year         = {2012},
}

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