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The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion

Angela K Spartz, Sang H Lee, onathan JP Wenger, Nathalie Gonzalez Sanchez UGent, Hironori Itoh, Dirk Inzé UGent, Wendy A Peer, Angus S Murphy, Paul J Overvoorde and William M Gray (2012) PLANT JOURNAL. 70(6). p.978-990
abstract
The plant hormone auxin controls numerous aspects of plant growth and development by regulating the expression of hundreds of genes. SMALL AUXIN UP RNA (SAUR) genes comprise the largest family of auxin-responsive genes, but their function is unknown. Although prior studies have correlated the expression of some SAUR genes with auxin-mediated cell expansion, genetic evidence implicating SAURs in cell expansion has not been reported. The Arabidopsis SAUR19, SAUR20, SAUR21, SAUR22, SAUR23, and SAUR24 (SAUR1924) genes encode a subgroup of closely related SAUR proteins. We demonstrate that these SAUR proteins are highly unstable in Arabidopsis. However, the addition of an N-terminal GFP or epitope tag dramatically increases the stability of SAUR proteins. Expression of these stabilized SAUR fusion proteins in Arabidopsis confers numerous auxin-related phenotypes indicative of increased and/or unregulated cell expansion, including increased hypocotyl and leaf size, defective apical hook maintenance, and altered tropic responses. Furthermore, seedlings expressing an artificial microRNA targeting multiple members of the SAUR1924 subfamily exhibit short hypocotyls and reduced leaf size. Together, these findings demonstrate that SAUR1924 function as positive effectors of cell expansion. This regulation may be achieved through the modulation of auxin transport, as SAUR gain-of-function and loss-of-function seedlings exhibit increased and reduced basipetal indole-3-acetic acid transport, respectively. Consistent with this possibility, SAUR1924 proteins predominantly localize to the plasma membrane.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
APICAL HOOK DEVELOPMENT, BOX PROTEIN TIR1, BINDING CASSETTE TRANSPORTER, N-TERMINAL UBIQUITINATION, auxin transport, protein stability, cell expansion, Arabidopsis, SAUR, auxin, ARABIDOPSIS-THALIANA, HYPOCOTYL ELONGATION, ARTIFICIAL MICRORNAS, DEGRADATION, GROWTH, PROTEASOME
journal title
PLANT JOURNAL
Plant J.
volume
70
issue
6
pages
978 - 990
Web of Science type
Article
Web of Science id
000305120400007
JCR category
PLANT SCIENCES
JCR impact factor
6.582 (2012)
JCR rank
7/193 (2012)
JCR quartile
1 (2012)
ISSN
0960-7412
DOI
10.1111/j.1365-313X.2012.04946.x
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2965468
handle
http://hdl.handle.net/1854/LU-2965468
date created
2012-07-30 13:55:29
date last changed
2014-05-26 10:04:11
@article{2965468,
  abstract     = {The plant hormone auxin controls numerous aspects of plant growth and development by regulating the expression of hundreds of genes. SMALL AUXIN UP RNA (SAUR) genes comprise the largest family of auxin-responsive genes, but their function is unknown. Although prior studies have correlated the expression of some SAUR genes with auxin-mediated cell expansion, genetic evidence implicating SAURs in cell expansion has not been reported. The Arabidopsis SAUR19, SAUR20, SAUR21, SAUR22, SAUR23, and SAUR24 (SAUR1924) genes encode a subgroup of closely related SAUR proteins. We demonstrate that these SAUR proteins are highly unstable in Arabidopsis. However, the addition of an N-terminal GFP or epitope tag dramatically increases the stability of SAUR proteins. Expression of these stabilized SAUR fusion proteins in Arabidopsis confers numerous auxin-related phenotypes indicative of increased and/or unregulated cell expansion, including increased hypocotyl and leaf size, defective apical hook maintenance, and altered tropic responses. Furthermore, seedlings expressing an artificial microRNA targeting multiple members of the SAUR1924 subfamily exhibit short hypocotyls and reduced leaf size. Together, these findings demonstrate that SAUR1924 function as positive effectors of cell expansion. This regulation may be achieved through the modulation of auxin transport, as SAUR gain-of-function and loss-of-function seedlings exhibit increased and reduced basipetal indole-3-acetic acid transport, respectively. Consistent with this possibility, SAUR1924 proteins predominantly localize to the plasma membrane.},
  author       = {Spartz, Angela K and Lee, Sang H and Wenger, onathan JP and Gonzalez Sanchez, Nathalie and Itoh, Hironori and Inz{\'e}, Dirk and Peer, Wendy A and Murphy, Angus S and Overvoorde, Paul J and Gray, William M},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {APICAL HOOK DEVELOPMENT,BOX PROTEIN TIR1,BINDING CASSETTE TRANSPORTER,N-TERMINAL UBIQUITINATION,auxin transport,protein stability,cell expansion,Arabidopsis,SAUR,auxin,ARABIDOPSIS-THALIANA,HYPOCOTYL ELONGATION,ARTIFICIAL MICRORNAS,DEGRADATION,GROWTH,PROTEASOME},
  language     = {eng},
  number       = {6},
  pages        = {978--990},
  title        = {The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion},
  url          = {http://dx.doi.org/10.1111/j.1365-313X.2012.04946.x},
  volume       = {70},
  year         = {2012},
}

Chicago
Spartz, Angela K, Sang H Lee, onathan JP Wenger, Nathalie Gonzalez Sanchez, Hironori Itoh, Dirk Inzé, Wendy A Peer, Angus S Murphy, Paul J Overvoorde, and William M Gray. 2012. “The SAUR19 Subfamily of SMALL AUXIN UP RNA Genes Promote Cell Expansion.” Plant Journal 70 (6): 978–990.
APA
Spartz, A. K., Lee, S. H., Wenger, onathan J., Gonzalez Sanchez, N., Itoh, H., Inzé, D., Peer, W. A., et al. (2012). The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion. PLANT JOURNAL, 70(6), 978–990.
Vancouver
1.
Spartz AK, Lee SH, Wenger onathan J, Gonzalez Sanchez N, Itoh H, Inzé D, et al. The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion. PLANT JOURNAL. 2012;70(6):978–90.
MLA
Spartz, Angela K, Sang H Lee, onathan JP Wenger, et al. “The SAUR19 Subfamily of SMALL AUXIN UP RNA Genes Promote Cell Expansion.” PLANT JOURNAL 70.6 (2012): 978–990. Print.