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Chemical PARP inhibition enhances growth of Arabidopsis and reduces anthocyanin accumulation and the activation of stress protective mechanisms

Philipp Schulz, Jenny Neukermans, Katrien Van Der Kelen UGent, Per Mühlenbock UGent, Frank Van Breusegem UGent, Graham Noctor, Markus Teige, Michael Metzlaff and Matthew A Hannah (2012) PLOS ONE. 7(5).
abstract
Poly-ADP-ribose polymerase (PARP) post-translationally modifies proteins through the addition of ADP-ribose polymers, yet its role in modulating plant development and stress responses is only poorly understood. The experiments presented here address some of the gaps in our understanding of its role in stress tolerance and thereby provide new insights into tolerance mechanisms and growth. Using a combination of chemical and genetic approaches, this study characterized phenotypes associated with PARP inhibition at the physiological level. Molecular analyses including gene expression analysis, measurement of primary metabolites and redox metabolites were used to understand the underlying processes. The analysis revealed that PARP inhibition represses anthocyanin and ascorbate accumulation under stress conditions. The reduction in defense is correlated with enhanced biomass production. Even in unstressed conditions protective genes and molecules are repressed by PARP inhibition. The reduced anthocyanin production was shown to be based on the repression of transcription of key regulatory and biosynthesis genes. PARP is a key factor for understanding growth and stress responses of plants. PARP inhibition allows plants to reduce protection such as anthocyanin, ascorbate or Non-Photochemical-Quenching whilst maintaining high energy levels likely enabling the observed enhancement of biomass production under stress, opening interesting perspectives for increasing crop productivity.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SUCROSE-SPECIFIC INDUCTION, POLY(ADP-RIBOSE) POLYMERASE, OXIDATIVE STRESS, GENE-EXPRESSION, CELL-DEATH, ADP-RIBOSYLATION, DROUGHT STRESS, REDOX STATE, HIGH LIGHT, SECONDARY METABOLISM
journal title
PLOS ONE
PLoS One
volume
7
issue
5
article_number
e37287
pages
13 pages
Web of Science type
Article
Web of Science id
000305342300031
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.73 (2012)
JCR rank
7/56 (2012)
JCR quartile
1 (2012)
ISSN
1932-6203
DOI
10.1371/journal.pone.0037287
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2965426
handle
http://hdl.handle.net/1854/LU-2965426
date created
2012-07-30 13:50:11
date last changed
2014-05-26 10:04:09
@article{2965426,
  abstract     = {Poly-ADP-ribose polymerase (PARP) post-translationally modifies proteins through the addition of ADP-ribose polymers, yet its role in modulating plant development and stress responses is only poorly understood. The experiments presented here address some of the gaps in our understanding of its role in stress tolerance and thereby provide new insights into tolerance mechanisms and growth. Using a combination of chemical and genetic approaches, this study characterized phenotypes associated with PARP inhibition at the physiological level. Molecular analyses including gene expression analysis, measurement of primary metabolites and redox metabolites were used to understand the underlying processes. The analysis revealed that PARP inhibition represses anthocyanin and ascorbate accumulation under stress conditions. The reduction in defense is correlated with enhanced biomass production. Even in unstressed conditions protective genes and molecules are repressed by PARP inhibition. The reduced anthocyanin production was shown to be based on the repression of transcription of key regulatory and biosynthesis genes. PARP is a key factor for understanding growth and stress responses of plants. PARP inhibition allows plants to reduce protection such as anthocyanin, ascorbate or Non-Photochemical-Quenching whilst maintaining high energy levels likely enabling the observed enhancement of biomass production under stress, opening interesting perspectives for increasing crop productivity.},
  articleno    = {e37287},
  author       = {Schulz, Philipp and Neukermans, Jenny and Van Der Kelen, Katrien and M{\"u}hlenbock, Per and Van Breusegem, Frank and Noctor, Graham and Teige, Markus and Metzlaff, Michael and Hannah, Matthew A},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {SUCROSE-SPECIFIC INDUCTION,POLY(ADP-RIBOSE) POLYMERASE,OXIDATIVE STRESS,GENE-EXPRESSION,CELL-DEATH,ADP-RIBOSYLATION,DROUGHT STRESS,REDOX STATE,HIGH LIGHT,SECONDARY METABOLISM},
  language     = {eng},
  number       = {5},
  pages        = {13},
  title        = {Chemical PARP inhibition enhances growth of Arabidopsis and reduces anthocyanin accumulation and the activation of stress protective mechanisms},
  url          = {http://dx.doi.org/10.1371/journal.pone.0037287},
  volume       = {7},
  year         = {2012},
}

Chicago
Schulz, Philipp, Jenny Neukermans, Katrien Van Der Kelen, Per Mühlenbock, Frank Van Breusegem, Graham Noctor, Markus Teige, Michael Metzlaff, and Matthew A Hannah. 2012. “Chemical PARP Inhibition Enhances Growth of Arabidopsis and Reduces Anthocyanin Accumulation and the Activation of Stress Protective Mechanisms.” Plos One 7 (5).
APA
Schulz, P., Neukermans, J., Van Der Kelen, K., Mühlenbock, P., Van Breusegem, F., Noctor, G., Teige, M., et al. (2012). Chemical PARP inhibition enhances growth of Arabidopsis and reduces anthocyanin accumulation and the activation of stress protective mechanisms. PLOS ONE, 7(5).
Vancouver
1.
Schulz P, Neukermans J, Van Der Kelen K, Mühlenbock P, Van Breusegem F, Noctor G, et al. Chemical PARP inhibition enhances growth of Arabidopsis and reduces anthocyanin accumulation and the activation of stress protective mechanisms. PLOS ONE. 2012;7(5).
MLA
Schulz, Philipp, Jenny Neukermans, Katrien Van Der Kelen, et al. “Chemical PARP Inhibition Enhances Growth of Arabidopsis and Reduces Anthocyanin Accumulation and the Activation of Stress Protective Mechanisms.” PLOS ONE 7.5 (2012): n. pag. Print.