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ROP GTPase-dependent actin microfilaments promote PIN1 polarization by localized inhibition of clathrin-dependent endocytosis

Shingo Nagawa, Tongda Xu, Deshu Lin, Pankaj Dhonukshe UGent, Xingxing Zhang, Jiri Friml UGent, Ben Scheres, Ying Fu and Zhenbiao Yang (2012) PLOS BIOLOGY. 10(4).
abstract
Cell polarization via asymmetrical distribution of structures or molecules is essential for diverse cellular functions and development of organisms, but how polarity is developmentally controlled has been poorly understood. In plants, the asymmetrical distribution of the PIN-FORMED (PIN) proteins involved in the cellular efflux of the quintessential phytohormone auxin plays a central role in developmental patterning, morphogenesis, and differential growth. Recently we showed that auxin promotes cell interdigitation by activating the Rho family ROP GTPases in leaf epidermal pavement cells. Here we found that auxin activation of the ROP2 signaling pathway regulates the asymmetric distribution of PIN1 by inhibiting its endocytosis. ROP2 inhibits PIN1 endocytosis via the accumulation of cortical actin microfilaments induced by the ROP2 effector protein RIC4. Our findings suggest a link between the developmental auxin signal and polar PIN1 distribution via Rho-dependent cytoskeletal reorganization and reveal the conservation of a design principle for cell polarization that is based on Rho GTPase-mediated inhibition of endocytosis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PROTEINS, POLAR AUXIN TRANSPORT, DYNAMICS, LINKS CELL POLARITY, ARABIDOPSIS-THALIANA, GENE-EXPRESSION, TIP GROWTH, POLLEN TUBES, F-ACTIN, MEDIATED ENDOCYTOSIS
journal title
PLOS BIOLOGY
PLoS. Biol.
volume
10
issue
4
article_number
e1001299
pages
11 pages
Web of Science type
Article
Web of Science id
000303541800005
ISSN
1544-9173
DOI
10.1371/journal.pbio.1001299
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2965025
handle
http://hdl.handle.net/1854/LU-2965025
date created
2012-07-30 13:03:17
date last changed
2013-09-17 11:15:57
@article{2965025,
  abstract     = {Cell polarization via asymmetrical distribution of structures or molecules is essential for diverse cellular functions and development of organisms, but how polarity is developmentally controlled has been poorly understood. In plants, the asymmetrical distribution of the PIN-FORMED (PIN) proteins involved in the cellular efflux of the quintessential phytohormone auxin plays a central role in developmental patterning, morphogenesis, and differential growth. Recently we showed that auxin promotes cell interdigitation by activating the Rho family ROP GTPases in leaf epidermal pavement cells. Here we found that auxin activation of the ROP2 signaling pathway regulates the asymmetric distribution of PIN1 by inhibiting its endocytosis. ROP2 inhibits PIN1 endocytosis via the accumulation of cortical actin microfilaments induced by the ROP2 effector protein RIC4. Our findings suggest a link between the developmental auxin signal and polar PIN1 distribution via Rho-dependent cytoskeletal reorganization and reveal the conservation of a design principle for cell polarization that is based on Rho GTPase-mediated inhibition of endocytosis.},
  articleno    = {e1001299},
  author       = {Nagawa, Shingo and Xu, Tongda and Lin, Deshu and Dhonukshe, Pankaj and Zhang, Xingxing and Friml, Jiri and Scheres, Ben and Fu, Ying and Yang, Zhenbiao},
  issn         = {1544-9173},
  journal      = {PLOS BIOLOGY},
  keyword      = {PROTEINS,POLAR AUXIN TRANSPORT,DYNAMICS,LINKS CELL POLARITY,ARABIDOPSIS-THALIANA,GENE-EXPRESSION,TIP GROWTH,POLLEN TUBES,F-ACTIN,MEDIATED ENDOCYTOSIS},
  language     = {eng},
  number       = {4},
  pages        = {11},
  title        = {ROP GTPase-dependent actin microfilaments promote PIN1 polarization by localized inhibition of clathrin-dependent endocytosis},
  url          = {http://dx.doi.org/10.1371/journal.pbio.1001299},
  volume       = {10},
  year         = {2012},
}

Chicago
Nagawa, Shingo, Tongda Xu, Deshu Lin, Pankaj Dhonukshe, Xingxing Zhang, Jiri Friml, Ben Scheres, Ying Fu, and Zhenbiao Yang. 2012. “ROP GTPase-dependent Actin Microfilaments Promote PIN1 Polarization by Localized Inhibition of Clathrin-dependent Endocytosis.” Plos Biology 10 (4).
APA
Nagawa, S., Xu, T., Lin, D., Dhonukshe, P., Zhang, X., Friml, J., Scheres, B., et al. (2012). ROP GTPase-dependent actin microfilaments promote PIN1 polarization by localized inhibition of clathrin-dependent endocytosis. PLOS BIOLOGY, 10(4).
Vancouver
1.
Nagawa S, Xu T, Lin D, Dhonukshe P, Zhang X, Friml J, et al. ROP GTPase-dependent actin microfilaments promote PIN1 polarization by localized inhibition of clathrin-dependent endocytosis. PLOS BIOLOGY. 2012;10(4).
MLA
Nagawa, Shingo, Tongda Xu, Deshu Lin, et al. “ROP GTPase-dependent Actin Microfilaments Promote PIN1 Polarization by Localized Inhibition of Clathrin-dependent Endocytosis.” PLOS BIOLOGY 10.4 (2012): n. pag. Print.