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ROP GTPase-dependent actin microfilaments promote PIN1 polarization by localized inhibition of clathrin-dependent endocytosis

(2012) PLOS BIOLOGY. 10(4).
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Abstract
Cell polarization via asymmetrical distribution of structures or molecules is essential for diverse cellular functions and development of organisms, but how polarity is developmentally controlled has been poorly understood. In plants, the asymmetrical distribution of the PIN-FORMED (PIN) proteins involved in the cellular efflux of the quintessential phytohormone auxin plays a central role in developmental patterning, morphogenesis, and differential growth. Recently we showed that auxin promotes cell interdigitation by activating the Rho family ROP GTPases in leaf epidermal pavement cells. Here we found that auxin activation of the ROP2 signaling pathway regulates the asymmetric distribution of PIN1 by inhibiting its endocytosis. ROP2 inhibits PIN1 endocytosis via the accumulation of cortical actin microfilaments induced by the ROP2 effector protein RIC4. Our findings suggest a link between the developmental auxin signal and polar PIN1 distribution via Rho-dependent cytoskeletal reorganization and reveal the conservation of a design principle for cell polarization that is based on Rho GTPase-mediated inhibition of endocytosis.
Keywords
PROTEINS, POLAR AUXIN TRANSPORT, DYNAMICS, LINKS CELL POLARITY, ARABIDOPSIS-THALIANA, GENE-EXPRESSION, TIP GROWTH, POLLEN TUBES, F-ACTIN, MEDIATED ENDOCYTOSIS

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Chicago
Nagawa, Shingo, Tongda Xu, Deshu Lin, Pankaj Dhonukshe, Xingxing Zhang, Jiri Friml, Ben Scheres, Ying Fu, and Zhenbiao Yang. 2012. “ROP GTPase-dependent Actin Microfilaments Promote PIN1 Polarization by Localized Inhibition of Clathrin-dependent Endocytosis.” Plos Biology 10 (4).
APA
Nagawa, S., Xu, T., Lin, D., Dhonukshe, P., Zhang, X., Friml, J., Scheres, B., et al. (2012). ROP GTPase-dependent actin microfilaments promote PIN1 polarization by localized inhibition of clathrin-dependent endocytosis. PLOS BIOLOGY, 10(4).
Vancouver
1.
Nagawa S, Xu T, Lin D, Dhonukshe P, Zhang X, Friml J, et al. ROP GTPase-dependent actin microfilaments promote PIN1 polarization by localized inhibition of clathrin-dependent endocytosis. PLOS BIOLOGY. 2012;10(4).
MLA
Nagawa, Shingo, Tongda Xu, Deshu Lin, et al. “ROP GTPase-dependent Actin Microfilaments Promote PIN1 Polarization by Localized Inhibition of Clathrin-dependent Endocytosis.” PLOS BIOLOGY 10.4 (2012): n. pag. Print.
@article{2965025,
  abstract     = {Cell polarization via asymmetrical distribution of structures or molecules is essential for diverse cellular functions and development of organisms, but how polarity is developmentally controlled has been poorly understood. In plants, the asymmetrical distribution of the PIN-FORMED (PIN) proteins involved in the cellular efflux of the quintessential phytohormone auxin plays a central role in developmental patterning, morphogenesis, and differential growth. Recently we showed that auxin promotes cell interdigitation by activating the Rho family ROP GTPases in leaf epidermal pavement cells. Here we found that auxin activation of the ROP2 signaling pathway regulates the asymmetric distribution of PIN1 by inhibiting its endocytosis. ROP2 inhibits PIN1 endocytosis via the accumulation of cortical actin microfilaments induced by the ROP2 effector protein RIC4. Our findings suggest a link between the developmental auxin signal and polar PIN1 distribution via Rho-dependent cytoskeletal reorganization and reveal the conservation of a design principle for cell polarization that is based on Rho GTPase-mediated inhibition of endocytosis.},
  articleno    = {e1001299},
  author       = {Nagawa, Shingo and Xu, Tongda and Lin, Deshu and Dhonukshe, Pankaj and Zhang, Xingxing and Friml, Jiri and Scheres, Ben and Fu, Ying and Yang, Zhenbiao},
  issn         = {1544-9173},
  journal      = {PLOS BIOLOGY},
  keyword      = {PROTEINS,POLAR AUXIN TRANSPORT,DYNAMICS,LINKS CELL POLARITY,ARABIDOPSIS-THALIANA,GENE-EXPRESSION,TIP GROWTH,POLLEN TUBES,F-ACTIN,MEDIATED ENDOCYTOSIS},
  language     = {eng},
  number       = {4},
  pages        = {11},
  title        = {ROP GTPase-dependent actin microfilaments promote PIN1 polarization by localized inhibition of clathrin-dependent endocytosis},
  url          = {http://dx.doi.org/10.1371/journal.pbio.1001299},
  volume       = {10},
  year         = {2012},
}

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