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Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes

Zoltán Magyar, Beatrix Horváth, Safina Khan, Binish Mohammed, Rossana Henriques, Lieven De Veylder UGent, László Bakó, Ben Scheres and László Bögre (2012) EMBO JOURNAL. 31(6). p.1480-1493
abstract
Post-embryonic growth in plants depends on the continuous supply of undifferentiated cells within meristems. Proliferating cells maintain their competence for division by active repression of differentiation and the associated endocycle entry. We show by upregulation and downregulation of E2FA that it is required for maintaining proliferation, as well as for endocycle entry. While E2FB-RBR1 (retinoblastoma-related protein 1) complexes are reduced after sucrose addition or at elevated CYCD3;1 levels, E2FA maintains a stable complex with RBR1 in proliferating cells. Chromatin immunoprecipitation shows that RBR1 binds in the proximity of E2F promoter elements in CCS52A1 and CSS52A2 genes, central regulators for the switch from proliferation to endocycles. Overexpression of a truncated E2FA mutant (E2FA(Delta RB)) lacking the RBR1-binding domain interferes with RBR1 recruitment to promoters through E2FA, leading to decreased meristem size in roots, premature cell expansion and hyperactivated endocycle in leaves. E2F target genes, including CCS52A1 and CCS52A2, are upregulated in E2FADRB and e2fa knockout lines. These data suggest that E2FA in complex with RBR1 forms a repressor complex in proliferating cells to inhibit premature differentiation and endocycle entry. Thus, E2FA regulates organ growth via two distinct, sequentially operating pathways.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
FOLLICLE CELLS, PLANT DEVELOPMENT, DNA-REPLICATION, A-TYPE CYCLIN, TRANSCRIPTION FACTOR, LEAF DEVELOPMENT, STEM-CELL MAINTENANCE, GENOME-WIDE IDENTIFICATION, RETINOBLASTOMA-RELATED PROTEIN, CYCLIN-DEPENDENT KINASE, retinoblastoma, E2F, endocycle, cell proliferation, Arabidopsis
journal title
EMBO JOURNAL
Embo J.
volume
31
issue
6
pages
1480 - 1493
Web of Science type
Article
Web of Science id
000302131600013
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
9.822 (2012)
JCR rank
17/288 (2012)
JCR quartile
1 (2012)
ISSN
0261-4189
DOI
10.1038/emboj.2012.13
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2965004
handle
http://hdl.handle.net/1854/LU-2965004
date created
2012-07-30 12:55:52
date last changed
2012-08-08 14:50:56
@article{2965004,
  abstract     = {Post-embryonic growth in plants depends on the continuous supply of undifferentiated cells within meristems. Proliferating cells maintain their competence for division by active repression of differentiation and the associated endocycle entry. We show by upregulation and downregulation of E2FA that it is required for maintaining proliferation, as well as for endocycle entry. While E2FB-RBR1 (retinoblastoma-related protein 1) complexes are reduced after sucrose addition or at elevated CYCD3;1 levels, E2FA maintains a stable complex with RBR1 in proliferating cells. Chromatin immunoprecipitation shows that RBR1 binds in the proximity of E2F promoter elements in CCS52A1 and CSS52A2 genes, central regulators for the switch from proliferation to endocycles. Overexpression of a truncated E2FA mutant (E2FA(Delta RB)) lacking the RBR1-binding domain interferes with RBR1 recruitment to promoters through E2FA, leading to decreased meristem size in roots, premature cell expansion and hyperactivated endocycle in leaves. E2F target genes, including CCS52A1 and CCS52A2, are upregulated in E2FADRB and e2fa knockout lines. These data suggest that E2FA in complex with RBR1 forms a repressor complex in proliferating cells to inhibit premature differentiation and endocycle entry. Thus, E2FA regulates organ growth via two distinct, sequentially operating pathways.},
  author       = {Magyar, Zolt{\'a}n and Horv{\'a}th, Beatrix and Khan, Safina and Mohammed, Binish and Henriques, Rossana and De Veylder, Lieven and Bak{\'o}, L{\'a}szl{\'o} and Scheres, Ben and B{\"o}gre, L{\'a}szl{\'o}},
  issn         = {0261-4189},
  journal      = {EMBO JOURNAL},
  keyword      = {FOLLICLE CELLS,PLANT DEVELOPMENT,DNA-REPLICATION,A-TYPE CYCLIN,TRANSCRIPTION FACTOR,LEAF DEVELOPMENT,STEM-CELL MAINTENANCE,GENOME-WIDE IDENTIFICATION,RETINOBLASTOMA-RELATED PROTEIN,CYCLIN-DEPENDENT KINASE,retinoblastoma,E2F,endocycle,cell proliferation,Arabidopsis},
  language     = {eng},
  number       = {6},
  pages        = {1480--1493},
  title        = {Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes},
  url          = {http://dx.doi.org/10.1038/emboj.2012.13},
  volume       = {31},
  year         = {2012},
}

Chicago
Magyar, Zoltán, Beatrix Horváth, Safina Khan, Binish Mohammed, Rossana Henriques, Lieven De Veylder, László Bakó, Ben Scheres, and László Bögre. 2012. “Arabidopsis E2FA Stimulates Proliferation and Endocycle Separately Through RBR-bound and RBR-free Complexes.” Embo Journal 31 (6): 1480–1493.
APA
Magyar, Z., Horváth, B., Khan, S., Mohammed, B., Henriques, R., De Veylder, L., Bakó, L., et al. (2012). Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes. EMBO JOURNAL, 31(6), 1480–1493.
Vancouver
1.
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, et al. Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes. EMBO JOURNAL. 2012;31(6):1480–93.
MLA
Magyar, Zoltán, Beatrix Horváth, Safina Khan, et al. “Arabidopsis E2FA Stimulates Proliferation and Endocycle Separately Through RBR-bound and RBR-free Complexes.” EMBO JOURNAL 31.6 (2012): 1480–1493. Print.