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Fluorescent castasterone reveals BRI1 signaling from the plasma membrane

(2012) NATURE CHEMICAL BIOLOGY. 8(6). p.583-589
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Abstract
Receptor-mediated endocytosis is an integral part of signal transduction as it mediates signal attenuation and provides spatial and temporal dimensions to signaling events. One of the best-studied leucine-rich repeat receptor-like kinases in plants, BRASSINOSTEROID INSENSITIVE 1 (BRI1), perceives its ligand, the brassinosteroid (BR) hormone, at the cell surface and is constitutively endocytosed. However, the importance of endocytosis for BR signaling remains unclear. Here we developed a bioactive, fluorescent BR analog, Alexa Fluor 647-castasterone (AFCS), and visualized the endocytosis of BRI1-AFCS complexes in living Arabidopsis thaliana cells. Impairment of endocytosis dependent on clathrin and the guanine nucleotide exchange factor for ARF GTPases (ARF-GEF) GNOM enhanced BR signaling by retaining active BRI1-ligand complexes at the plasma membrane. Increasing the trans-Golgi network/early endosome pool of BRI1-BR complexes did not affect BR signaling. Our findings provide what is to our knowledge the first visualization of receptor-ligand complexes in plants and reveal clathrin- and ARF-GEF-dependent endocytic regulation of BR signaling from the plasma membrane.
Keywords
ARABIDOPSIS, CLATHRIN, AUXIN TRANSPORT, DEPENDENT ENDOCYTOSIS, BRASSINOSTEROID BIOSYNTHESIS, PLANT-CELLS, STEROID-BINDING PROTEIN-1, RECEPTOR KINASE BRI1, TRANSDUCTION, INHIBITION

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MLA
Irani, Niloufer, Simone Di Rubbo, Evelien Mylle, et al. “Fluorescent Castasterone Reveals BRI1 Signaling from the Plasma Membrane.” NATURE CHEMICAL BIOLOGY 8.6 (2012): 583–589. Print.
APA
Irani, N., Di Rubbo, S., Mylle, E., Van den Begin, J., Pizon, J., Hniliková, J., Šiša, M., et al. (2012). Fluorescent castasterone reveals BRI1 signaling from the plasma membrane. NATURE CHEMICAL BIOLOGY, 8(6), 583–589.
Chicago author-date
Irani, Niloufer, Simone Di Rubbo, Evelien Mylle, Jos Van den Begin, Joanna Pizon, Jaroslava Hniliková, Miroslav Šiša, et al. 2012. “Fluorescent Castasterone Reveals BRI1 Signaling from the Plasma Membrane.” Nature Chemical Biology 8 (6): 583–589.
Chicago author-date (all authors)
Irani, Niloufer, Simone Di Rubbo, Evelien Mylle, Jos Van den Begin, Joanna Pizon, Jaroslava Hniliková, Miroslav Šiša, Dieter Buyst, Josep Vilarrasa-Blasi, Anna Maria Szatmari, Daniël Van Damme, Kiril Mishev, Mirela-Corina Codreanu, Ladislav Kohout, Miroslav Strnad, Ana I Caño-Delgado, Jiri Friml, Annemieke Madder, and Eugenia Russinova. 2012. “Fluorescent Castasterone Reveals BRI1 Signaling from the Plasma Membrane.” Nature Chemical Biology 8 (6): 583–589.
Vancouver
1.
Irani N, Di Rubbo S, Mylle E, Van den Begin J, Pizon J, Hniliková J, et al. Fluorescent castasterone reveals BRI1 signaling from the plasma membrane. NATURE CHEMICAL BIOLOGY. 2012;8(6):583–9.
IEEE
[1]
N. Irani et al., “Fluorescent castasterone reveals BRI1 signaling from the plasma membrane,” NATURE CHEMICAL BIOLOGY, vol. 8, no. 6, pp. 583–589, 2012.
@article{2964959,
  abstract     = {{Receptor-mediated endocytosis is an integral part of signal transduction as it mediates signal attenuation and provides spatial and temporal dimensions to signaling events. One of the best-studied leucine-rich repeat receptor-like kinases in plants, BRASSINOSTEROID INSENSITIVE 1 (BRI1), perceives its ligand, the brassinosteroid (BR) hormone, at the cell surface and is constitutively endocytosed. However, the importance of endocytosis for BR signaling remains unclear. Here we developed a bioactive, fluorescent BR analog, Alexa Fluor 647-castasterone (AFCS), and visualized the endocytosis of BRI1-AFCS complexes in living Arabidopsis thaliana cells. Impairment of endocytosis dependent on clathrin and the guanine nucleotide exchange factor for ARF GTPases (ARF-GEF) GNOM enhanced BR signaling by retaining active BRI1-ligand complexes at the plasma membrane. Increasing the trans-Golgi network/early endosome pool of BRI1-BR complexes did not affect BR signaling. Our findings provide what is to our knowledge the first visualization of receptor-ligand complexes in plants and reveal clathrin- and ARF-GEF-dependent endocytic regulation of BR signaling from the plasma membrane.}},
  author       = {{Irani, Niloufer and Di Rubbo, Simone and Mylle, Evelien and Van den Begin, Jos and Pizon, Joanna and Hniliková, Jaroslava and Šiša, Miroslav and Buyst, Dieter and Vilarrasa-Blasi, Josep and Szatmari, Anna Maria and Van Damme, Daniël and Mishev, Kiril and Codreanu, Mirela-Corina and Kohout, Ladislav and Strnad, Miroslav and Caño-Delgado, Ana I and Friml, Jiri and Madder, Annemieke and Russinova, Eugenia}},
  issn         = {{1552-4450}},
  journal      = {{NATURE CHEMICAL BIOLOGY}},
  keywords     = {{ARABIDOPSIS,CLATHRIN,AUXIN TRANSPORT,DEPENDENT ENDOCYTOSIS,BRASSINOSTEROID BIOSYNTHESIS,PLANT-CELLS,STEROID-BINDING PROTEIN-1,RECEPTOR KINASE BRI1,TRANSDUCTION,INHIBITION}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{583--589}},
  title        = {{Fluorescent castasterone reveals BRI1 signaling from the plasma membrane}},
  url          = {{http://dx.doi.org/10.1038/NCHEMBIO.958}},
  volume       = {{8}},
  year         = {{2012}},
}

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