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Fluorescent castasterone reveals BRI1 signaling from the plasma membrane

Niloufer Irani UGent, Simone Di Rubbo UGent, Evelien Mylle UGent, Jos Van den Begin UGent, Joanna Pizon UGent, Jaroslava Hniliková, Miroslav Šiša, Dieter Buyst UGent, Josep Vilarrasa-Blasi and Anna Maria Szatmari UGent, et al. (2012) NATURE CHEMICAL BIOLOGY. 8(6). p.583-589
abstract
Receptor-mediated endocytosis is an integral part of signal transduction as it mediates signal attenuation and provides spatial and temporal dimensions to signaling events. One of the best-studied leucine-rich repeat receptor-like kinases in plants, BRASSINOSTEROID INSENSITIVE 1 (BRI1), perceives its ligand, the brassinosteroid (BR) hormone, at the cell surface and is constitutively endocytosed. However, the importance of endocytosis for BR signaling remains unclear. Here we developed a bioactive, fluorescent BR analog, Alexa Fluor 647-castasterone (AFCS), and visualized the endocytosis of BRI1-AFCS complexes in living Arabidopsis thaliana cells. Impairment of endocytosis dependent on clathrin and the guanine nucleotide exchange factor for ARF GTPases (ARF-GEF) GNOM enhanced BR signaling by retaining active BRI1-ligand complexes at the plasma membrane. Increasing the trans-Golgi network/early endosome pool of BRI1-BR complexes did not affect BR signaling. Our findings provide what is to our knowledge the first visualization of receptor-ligand complexes in plants and reveal clathrin- and ARF-GEF-dependent endocytic regulation of BR signaling from the plasma membrane.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ARABIDOPSIS, CLATHRIN, AUXIN TRANSPORT, DEPENDENT ENDOCYTOSIS, BRASSINOSTEROID BIOSYNTHESIS, PLANT-CELLS, STEROID-BINDING PROTEIN-1, RECEPTOR KINASE BRI1, TRANSDUCTION, INHIBITION
journal title
NATURE CHEMICAL BIOLOGY
Nat. Chem. Biol.
volume
8
issue
6
pages
583 - 589
Web of Science type
Article
Web of Science id
000304256300015
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
12.948 (2012)
JCR rank
9/288 (2012)
JCR quartile
1 (2012)
ISSN
1552-4450
DOI
10.1038/NCHEMBIO.958
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2964959
handle
http://hdl.handle.net/1854/LU-2964959
date created
2012-07-30 12:14:59
date last changed
2012-08-08 13:57:51
@article{2964959,
  abstract     = {Receptor-mediated endocytosis is an integral part of signal transduction as it mediates signal attenuation and provides spatial and temporal dimensions to signaling events. One of the best-studied leucine-rich repeat receptor-like kinases in plants, BRASSINOSTEROID INSENSITIVE 1 (BRI1), perceives its ligand, the brassinosteroid (BR) hormone, at the cell surface and is constitutively endocytosed. However, the importance of endocytosis for BR signaling remains unclear. Here we developed a bioactive, fluorescent BR analog, Alexa Fluor 647-castasterone (AFCS), and visualized the endocytosis of BRI1-AFCS complexes in living Arabidopsis thaliana cells. Impairment of endocytosis dependent on clathrin and the guanine nucleotide exchange factor for ARF GTPases (ARF-GEF) GNOM enhanced BR signaling by retaining active BRI1-ligand complexes at the plasma membrane. Increasing the trans-Golgi network/early endosome pool of BRI1-BR complexes did not affect BR signaling. Our findings provide what is to our knowledge the first visualization of receptor-ligand complexes in plants and reveal clathrin- and ARF-GEF-dependent endocytic regulation of BR signaling from the plasma membrane.},
  author       = {Irani, Niloufer and Di Rubbo, Simone and Mylle, Evelien and Van den Begin, Jos and Pizon, Joanna and Hnilikov{\'a}, Jaroslava and \v{S}i\v{s}a, Miroslav and Buyst, Dieter and Vilarrasa-Blasi, Josep and Szatmari, Anna Maria and Van Damme, Dani{\"e}l and Mishev, Kiril and Codreanu, Mirela-Corina and Kohout, Ladislav and Strnad, Miroslav and Ca{\~n}o-Delgado, Ana I and Friml, Jiri and Madder, Annemieke and Russinova, Eugenia},
  issn         = {1552-4450},
  journal      = {NATURE CHEMICAL BIOLOGY},
  keyword      = {ARABIDOPSIS,CLATHRIN,AUXIN TRANSPORT,DEPENDENT ENDOCYTOSIS,BRASSINOSTEROID BIOSYNTHESIS,PLANT-CELLS,STEROID-BINDING PROTEIN-1,RECEPTOR KINASE BRI1,TRANSDUCTION,INHIBITION},
  language     = {eng},
  number       = {6},
  pages        = {583--589},
  title        = {Fluorescent castasterone reveals BRI1 signaling from the plasma membrane},
  url          = {http://dx.doi.org/10.1038/NCHEMBIO.958},
  volume       = {8},
  year         = {2012},
}

Chicago
Irani, Niloufer, Simone Di Rubbo, Evelien Mylle, Jos Van den Begin, Joanna Pizon, Jaroslava Hniliková, Miroslav Šiša, et al. 2012. “Fluorescent Castasterone Reveals BRI1 Signaling from the Plasma Membrane.” Nature Chemical Biology 8 (6): 583–589.
APA
Irani, N., Di Rubbo, S., Mylle, E., Van den Begin, J., Pizon, J., Hniliková, J., Šiša, M., et al. (2012). Fluorescent castasterone reveals BRI1 signaling from the plasma membrane. NATURE CHEMICAL BIOLOGY, 8(6), 583–589.
Vancouver
1.
Irani N, Di Rubbo S, Mylle E, Van den Begin J, Pizon J, Hniliková J, et al. Fluorescent castasterone reveals BRI1 signaling from the plasma membrane. NATURE CHEMICAL BIOLOGY. 2012;8(6):583–9.
MLA
Irani, Niloufer, Simone Di Rubbo, Evelien Mylle, et al. “Fluorescent Castasterone Reveals BRI1 Signaling from the Plasma Membrane.” NATURE CHEMICAL BIOLOGY 8.6 (2012): 583–589. Print.