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Construction of a genetic linkage map with SSR, AFLP and morphological markers to locate QTLs controlling pathotype-specific powdery mildew resistance in diploid roses

Hossein Hosseini Moghaddam UGent, Leen Leus UGent, Jan De Riek, Johan Van Huylenbroeck and Erik Van Bockstaele UGent (2012) EUPHYTICA. 184(3). p.413-427
abstract
Disease resistance is a sought-after trait in plant breeding programmes. One strategy to make resistance more durable is to increase the number of resistance genes, thereby increasing the number of pathotypes withstood. One of the most important diseases on roses is powdery mildew (PM) (Podosphaera pannosa). Recent studies show that pathotypes of PM and different types of resistances in roses exist. The results of this study aim to contribute to PM resistance in roses by the development of pathotype-specific markers on a genetic map. A diploid rose population (90 genotypes) derived from a cross between Rosa wichurana and Rosa 'Yesterday' was used to construct a genetic linkage map encompassing 20 AFLP primer combinations, 43 SSR, and 2 morphological markers. By applying the F1 pseudo test cross population strategy, two parental linkage maps were constructed (parent 'Yesterday' 536 cM; parent R. wichurana 526 cM). Both parental maps consisted of seven linkage groups with an average length of 70 cM (Kosambi) corresponding to the seven haploid rose chromosomes. These new maps were used to identify QTLs controlling disease resistance. The offspring population was screened for resistance to two PM pathotypes, R-E and R-P. QTLs for controlling pathotype-specific disease resistance were mapped by applying Kruskal-Wallis rank-sum tests and simple interval mapping. With two pathotypes analysed, nine QTL loci were detected on linkage groups 2, 3, 5 and 6, explaining 15-73% of the phenotypic variance for pathotype-specific disease response. The genetic maps developed here will be useful for future rose breeding, pathotype-specific resistance research and development of a consensus map for roses.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Disease resistance, Linkage mapping, Molecular markers, Pathotype-specific, QTL mapping, R. wichurana, QUANTITATIVE TRAIT LOCI, DISEASE RESISTANCE, ASSISTED SELECTION, SEGREGATION DISTORTION, MOLECULAR MARKERS, PLANT, RICE, MICROSATELLITE, POPULATION, VALIDATION
journal title
EUPHYTICA
Euphytica
volume
184
issue
3
pages
413 - 427
Web of Science type
Article
Web of Science id
000301783300012
JCR category
HORTICULTURE
JCR impact factor
1.643 (2012)
JCR rank
7/32 (2012)
JCR quartile
1 (2012)
ISSN
0014-2336
DOI
10.1007/s10681-011-0616-6
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2964527
handle
http://hdl.handle.net/1854/LU-2964527
date created
2012-07-27 09:48:42
date last changed
2014-05-26 10:03:50
@article{2964527,
  abstract     = {Disease resistance is a sought-after trait in plant breeding programmes. One strategy to make resistance more durable is to increase the number of resistance genes, thereby increasing the number of pathotypes withstood. One of the most important diseases on roses is powdery mildew (PM) (Podosphaera pannosa). Recent studies show that pathotypes of PM and different types of resistances in roses exist. The results of this study aim to contribute to PM resistance in roses by the development of pathotype-specific markers on a genetic map. A diploid rose population (90 genotypes) derived from a cross between Rosa wichurana and Rosa 'Yesterday' was used to construct a genetic linkage map encompassing 20 AFLP primer combinations, 43 SSR, and 2 morphological markers. By applying the F1 pseudo test cross population strategy, two parental linkage maps were constructed (parent 'Yesterday' 536 cM; parent R. wichurana 526 cM). Both parental maps consisted of seven linkage groups with an average length of 70 cM (Kosambi) corresponding to the seven haploid rose chromosomes. These new maps were used to identify QTLs controlling disease resistance. The offspring population was screened for resistance to two PM pathotypes, R-E and R-P. QTLs for controlling pathotype-specific disease resistance were mapped by applying Kruskal-Wallis rank-sum tests and simple interval mapping. With two pathotypes analysed, nine QTL loci were detected on linkage groups 2, 3, 5 and 6, explaining 15-73\% of the phenotypic variance for pathotype-specific disease response. The genetic maps developed here will be useful for future rose breeding, pathotype-specific resistance research and development of a consensus map for roses.},
  author       = {Hosseini Moghaddam, Hossein and Leus, Leen and De Riek, Jan and Van Huylenbroeck, Johan and Van Bockstaele, Erik},
  issn         = {0014-2336},
  journal      = {EUPHYTICA},
  keyword      = {Disease resistance,Linkage mapping,Molecular markers,Pathotype-specific,QTL mapping,R. wichurana,QUANTITATIVE TRAIT LOCI,DISEASE RESISTANCE,ASSISTED SELECTION,SEGREGATION DISTORTION,MOLECULAR MARKERS,PLANT,RICE,MICROSATELLITE,POPULATION,VALIDATION},
  language     = {eng},
  number       = {3},
  pages        = {413--427},
  title        = {Construction of a genetic linkage map with SSR, AFLP and morphological markers to locate QTLs controlling pathotype-specific powdery mildew resistance in diploid roses},
  url          = {http://dx.doi.org/10.1007/s10681-011-0616-6},
  volume       = {184},
  year         = {2012},
}

Chicago
Hosseini Moghaddam, Hossein, Leen Leus, Jan De Riek, Johan Van Huylenbroeck, and Erik Van Bockstaele. 2012. “Construction of a Genetic Linkage Map with SSR, AFLP and Morphological Markers to Locate QTLs Controlling Pathotype-specific Powdery Mildew Resistance in Diploid Roses.” Euphytica 184 (3): 413–427.
APA
Hosseini Moghaddam, H., Leus, L., De Riek, J., Van Huylenbroeck, J., & Van Bockstaele, E. (2012). Construction of a genetic linkage map with SSR, AFLP and morphological markers to locate QTLs controlling pathotype-specific powdery mildew resistance in diploid roses. EUPHYTICA, 184(3), 413–427.
Vancouver
1.
Hosseini Moghaddam H, Leus L, De Riek J, Van Huylenbroeck J, Van Bockstaele E. Construction of a genetic linkage map with SSR, AFLP and morphological markers to locate QTLs controlling pathotype-specific powdery mildew resistance in diploid roses. EUPHYTICA. 2012;184(3):413–27.
MLA
Hosseini Moghaddam, Hossein, Leen Leus, Jan De Riek, et al. “Construction of a Genetic Linkage Map with SSR, AFLP and Morphological Markers to Locate QTLs Controlling Pathotype-specific Powdery Mildew Resistance in Diploid Roses.” EUPHYTICA 184.3 (2012): 413–427. Print.