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Agroinfiltration of intact leaves as a method for the transient and stable transformation of saponin producing Maesa lanceolata

Ahmad Faizal UGent and Danny Geelen UGent (2012) PLANT CELL REPORTS. 31(8). p.1517-1526
abstract
A method has been developed to genetically transform the medicinal plant Maesa lanceolata. Initially we tested conditions for transient expression of GFP bearing constructs in agroinfiltrated leaves. Leaf tissues of M. lanceolata were infiltrated with Agrobacterium tumefaciens carrying a nuclear-targeted GFP-construct to allow the quantification of the transformation efficiency. The number of transfected cells depended on the bacterial density, bacterial strains, the co-cultivation time, and presence of acetosyringone. The transient transformation assay generated the highest ratio of transfected cells over non-transfected cells upon 5 days post infiltration using A. tumefaciens strain LBA4404 at an OD600 = 1.0 in the presence of 100 µM acetosyringone and in the absence of a viral suppressor construct. In a second series of experiments we set up a stable transformation protocol that resulted in the regeneration of kanamycin resistant plants expressing nuclear GFP. This transformation protocol will be used to introduce overexpression and RNAi constructs into M. lanceolata plants that may interfere with triterpenoid saponin biosynthesis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
AGROBACTERIUM-MEDIATED TRANSFORMATION, saponin, stable transformation, Maesa lanceolata, transient gene expression, Agroinfiltration, FUNCTIONAL GENE ASSAY, EXPRESSION SYSTEM, TRITERPENOID SAPONINS, PLANT TRANSFORMATION, VIRAL SUPPRESSORS, RAPID TOOL, IN-VIVO, ARABIDOPSIS, GRAPEVINE
journal title
PLANT CELL REPORTS
Plant Cell Reports
volume
31
issue
8
pages
1517 - 1526
Web of Science type
Article
Web of Science id
000306438200014
JCR category
PLANT SCIENCES
JCR impact factor
2.509 (2012)
JCR rank
49/193 (2012)
JCR quartile
2 (2012)
ISSN
0721-7714
DOI
10.1007/s00299-012-1266-4
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2964182
handle
http://hdl.handle.net/1854/LU-2964182
date created
2012-07-25 10:53:36
date last changed
2012-09-25 14:59:52
@article{2964182,
  abstract     = {A method has been developed to genetically transform the medicinal plant Maesa lanceolata. Initially we tested conditions for transient expression of GFP bearing constructs in agroinfiltrated leaves. Leaf tissues of M. lanceolata were infiltrated with Agrobacterium tumefaciens carrying a nuclear-targeted GFP-construct to allow the quantification of the transformation efficiency. The number of transfected cells depended on the bacterial density, bacterial strains, the co-cultivation time, and presence of acetosyringone. The transient transformation assay generated the highest ratio of transfected cells over non-transfected cells upon 5 days post infiltration using A. tumefaciens strain LBA4404 at an OD600 = 1.0 in the presence of 100 {\textmu}M acetosyringone and in the absence of a viral suppressor construct. In a second series of experiments we set up a stable transformation protocol that resulted in the regeneration of kanamycin resistant plants expressing nuclear GFP. This transformation protocol will be used to introduce overexpression and RNAi constructs into M. lanceolata plants that may interfere with triterpenoid saponin biosynthesis.},
  author       = {Faizal, Ahmad and Geelen, Danny},
  issn         = {0721-7714},
  journal      = {PLANT CELL REPORTS},
  keyword      = {AGROBACTERIUM-MEDIATED TRANSFORMATION,saponin,stable transformation,Maesa lanceolata,transient gene expression,Agroinfiltration,FUNCTIONAL GENE ASSAY,EXPRESSION SYSTEM,TRITERPENOID SAPONINS,PLANT TRANSFORMATION,VIRAL SUPPRESSORS,RAPID TOOL,IN-VIVO,ARABIDOPSIS,GRAPEVINE},
  language     = {eng},
  number       = {8},
  pages        = {1517--1526},
  title        = {Agroinfiltration of intact leaves as a method for the transient and stable transformation of saponin producing Maesa lanceolata},
  url          = {http://dx.doi.org/10.1007/s00299-012-1266-4},
  volume       = {31},
  year         = {2012},
}

Chicago
Faizal, Ahmad, and Danny Geelen. 2012. “Agroinfiltration of Intact Leaves as a Method for the Transient and Stable Transformation of Saponin Producing Maesa Lanceolata.” Plant Cell Reports 31 (8): 1517–1526.
APA
Faizal, A., & Geelen, D. (2012). Agroinfiltration of intact leaves as a method for the transient and stable transformation of saponin producing Maesa lanceolata. PLANT CELL REPORTS, 31(8), 1517–1526.
Vancouver
1.
Faizal A, Geelen D. Agroinfiltration of intact leaves as a method for the transient and stable transformation of saponin producing Maesa lanceolata. PLANT CELL REPORTS. 2012;31(8):1517–26.
MLA
Faizal, Ahmad, and Danny Geelen. “Agroinfiltration of Intact Leaves as a Method for the Transient and Stable Transformation of Saponin Producing Maesa Lanceolata.” PLANT CELL REPORTS 31.8 (2012): 1517–1526. Print.