Ghent University Academic Bibliography

Advanced

Regeneration ability and genetic transformation of root type chicory (Cichorium intybus var. sativum)

Asad Maroufi UGent, Mansour Karimi UGent, Khosro Mehdi Khanlou UGent, Erik Van Bockstaele UGent and Marc De Loose UGent (2012) AFRICAN JOURNAL OF BIOTECHNOLOGY. 11(56). p.11874-11886
abstract
To develop an efficient protocol for shoot regeneration of root chicory (Cichorium intybus var. sativum), some factors, including different concentrations of plant growth regulators in Murashige and Skoog (MS) medium, type of explants and genotypes were evaluated. Initiation of callusing were best achieved in MS medium supplemented with 1-naphthaleneacetic acid (NAA) (0.1 mg l-1) plus 6-Benzylaminopurine (6-BAP) (1 mg l-1), indole-3-acetic acid (IAA) (0.01 mg l-1) plus 6-BAP (1.0 mg l-1), and IAA (0.5 mg l-1) plus (0.5 mg l-1) 6-BAP combinations on leaf and cotyledon explants. Explant-derived calli were able to produce multiple adventitious shoots in MS medium containing IAA (0.5 mg l-1) plus 6-BAP (0.5 mg l-1). MS medium containing indole-3-butylric acid IBA (1 mgl-1) efficiently induced rooting on elongated shoots. Various responses to the number of generated shoots were observed when regeneration abilities of different chicory cultivars were examined. Among root and “Witloof” cultivars, ‘Melci’ and ‘Hera’ belong to the root cultivars and exhibited higher shoot regeneration ability. Using the optimized regeneration method, genetic transformation of ‘Melci’ with Agrobacterium tumefaciens strain C58C1 RifR (pGV2260) (pTJK136) was successfully carried out. Histochemical GUS assay, polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR) analysis of putative transformed plants confirmed successful integration of the T-DNA into the chicory genome. Expression of the neomycine phosphotransferase (NPTII) in the regenerated plants was also shown by well-developed roots on root inducing medium containing 100 mg l-1 kanamycin. This simple, efficient and reproducible protocol could be useful for inducing somaclonal variation and genetic modification of root chicory cultivars to broaden genetic variation and transferring of important genes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
regeneration, 6-Benzylaminopurine (6-BAP), Chicory, transformation, indole-3-acetic acid (IAA), 1-Naphthaleneacetic acid (NAA)
journal title
AFRICAN JOURNAL OF BIOTECHNOLOGY
Afr. J. Biotechnol.
volume
11
issue
56
pages
11874 - 11886
ISSN
1684-5315
DOI
10.5897/AJB11.438
language
English
UGent publication?
yes
classification
A2
copyright statement
I have transferred the copyright for this publication to the publisher
id
2963367
handle
http://hdl.handle.net/1854/LU-2963367
date created
2012-07-18 08:53:05
date last changed
2013-10-21 09:12:27
@article{2963367,
  abstract     = {To develop an efficient protocol for shoot regeneration of root chicory (Cichorium intybus var. sativum), some factors, including different concentrations of plant growth regulators in Murashige and Skoog (MS) medium, type of explants and genotypes were evaluated. Initiation of callusing were best achieved in MS medium supplemented with 1-naphthaleneacetic acid (NAA) (0.1 mg l-1) plus 6-Benzylaminopurine (6-BAP) (1 mg l-1), indole-3-acetic acid (IAA) (0.01 mg l-1) plus 6-BAP (1.0 mg l-1), and IAA (0.5 mg l-1) plus (0.5 mg l-1) 6-BAP combinations on leaf and cotyledon explants. Explant-derived calli were able to produce multiple adventitious shoots in MS medium containing IAA (0.5 mg l-1) plus 6-BAP (0.5 mg l-1). MS medium containing indole-3-butylric acid IBA (1 mgl-1) efficiently induced rooting on elongated shoots. Various responses to the number of generated shoots were observed when regeneration abilities of different chicory cultivars were examined. Among root and {\textquotedblleft}Witloof{\textquotedblright} cultivars, {\textquoteleft}Melci{\textquoteright} and {\textquoteleft}Hera{\textquoteright} belong to the root cultivars and exhibited higher shoot regeneration ability. Using the optimized regeneration method, genetic transformation of {\textquoteleft}Melci{\textquoteright} with Agrobacterium tumefaciens strain C58C1 RifR (pGV2260) (pTJK136) was successfully carried out. Histochemical GUS assay, polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR) analysis of putative transformed plants confirmed successful integration of the T-DNA into the chicory genome. Expression of the neomycine phosphotransferase (NPTII) in the regenerated plants was also shown by well-developed roots on root inducing medium containing 100 mg l-1 kanamycin. This simple, efficient and reproducible protocol could be useful for inducing somaclonal variation and genetic modification of root chicory cultivars to broaden genetic variation and transferring of important genes.},
  author       = {Maroufi, Asad and Karimi, Mansour and Mehdi Khanlou, Khosro and Van Bockstaele, Erik and De Loose, Marc},
  issn         = {1684-5315},
  journal      = {AFRICAN JOURNAL OF BIOTECHNOLOGY},
  keyword      = {regeneration,6-Benzylaminopurine (6-BAP),Chicory,transformation,indole-3-acetic acid (IAA),1-Naphthaleneacetic acid (NAA)},
  language     = {eng},
  number       = {56},
  pages        = {11874--11886},
  title        = {Regeneration ability and genetic transformation of root type chicory (Cichorium intybus var. sativum)},
  url          = {http://dx.doi.org/10.5897/AJB11.438},
  volume       = {11},
  year         = {2012},
}

Chicago
Maroufi, Asad, Mansour Karimi, Khosro Mehdi Khanlou, Erik Van Bockstaele, and Marc De Loose. 2012. “Regeneration Ability and Genetic Transformation of Root Type Chicory (Cichorium Intybus Var. Sativum).” African Journal of Biotechnology 11 (56): 11874–11886.
APA
Maroufi, A., Karimi, M., Mehdi Khanlou, K., Van Bockstaele, E., & De Loose, M. (2012). Regeneration ability and genetic transformation of root type chicory (Cichorium intybus var. sativum). AFRICAN JOURNAL OF BIOTECHNOLOGY, 11(56), 11874–11886.
Vancouver
1.
Maroufi A, Karimi M, Mehdi Khanlou K, Van Bockstaele E, De Loose M. Regeneration ability and genetic transformation of root type chicory (Cichorium intybus var. sativum). AFRICAN JOURNAL OF BIOTECHNOLOGY. 2012;11(56):11874–86.
MLA
Maroufi, Asad, Mansour Karimi, Khosro Mehdi Khanlou, et al. “Regeneration Ability and Genetic Transformation of Root Type Chicory (Cichorium Intybus Var. Sativum).” AFRICAN JOURNAL OF BIOTECHNOLOGY 11.56 (2012): 11874–11886. Print.