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GATA-4 and MEF2C transcription factors control the tissue-specific expression of the αT-catenin gene CTNNA3

Griet Vanpoucke, Steven Goossens (UGent) , Bram De Craene (UGent) , Barbara Gilbert (UGent) , Frans Van Roy (UGent) and Geert Berx (UGent)
(2004) NUCLEIC ACIDS RESEARCH. 32(14). p.4155-4165
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Abstract
alphaT-catenin is a recently identified member of the alpha-catenin family of cell-cell adhesion molecules. Its expression is restricted mainly to cardiomyocytes, although it is also expressed in skeletal muscle, testis and brain. Like other alpha-catenins, alphaT-catenin provides an indispensable link between a cadherin-based adhesion complex and the actin cytoskeleton, resulting in strong cell-cell adhesion. We show here that the tissue-specificity of alphaT-catenin expression is controlled by its promoter region. By in silico, analysis, we found that the alphaT-catenin promoter contains several binding sites for cardiac and muscle-specific transcription factors. By co-transfection studies in P19 embryonal carcinoma cells, we demonstrated that MEF2C and GATA-4 each have an activating effect on the alphaT-catenin promoter. Transfections with wildtype and mutant promoter constructs in cardiac HL-1 cells indicated that one GATA box is absolutely required for high alphaT-catenin promoter activity in these cells. Furthermore, we showed that the GATA-4 transcription factor specifically binds and activates the alphaT-catenin promoter in vivo in cardiac HL-1 cells. In vivo promoter analysis in transgenic mice revealed that the isolated alphaT-catenin promoter region could direct the tissue-specific expression of a LacZ reporter gene in concordance with endogenous alphaT-catenin expression.
Keywords
PROSTATE-CANCER CELLS, E-CADHERIN, ALZHEIMERS-DISEASE, HUMAN BRAIN, FACTOR 2C, PROTEIN, ADHESION, HEART, MORPHOGENESIS, PROMOTERS

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Citation

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MLA
Vanpoucke, Griet, Steven Goossens, Bram De Craene, et al. “GATA-4 and MEF2C Transcription Factors Control the Tissue-specific Expression of the αT-catenin Gene CTNNA3.” NUCLEIC ACIDS RESEARCH 32.14 (2004): 4155–4165. Print.
APA
Vanpoucke, G., Goossens, S., De Craene, B., Gilbert, B., Van Roy, F., & Berx, G. (2004). GATA-4 and MEF2C transcription factors control the tissue-specific expression of the αT-catenin gene CTNNA3. NUCLEIC ACIDS RESEARCH, 32(14), 4155–4165.
Chicago author-date
Vanpoucke, Griet, Steven Goossens, Bram De Craene, Barbara Gilbert, Frans Van Roy, and Geert Berx. 2004. “GATA-4 and MEF2C Transcription Factors Control the Tissue-specific Expression of the αT-catenin Gene CTNNA3.” Nucleic Acids Research 32 (14): 4155–4165.
Chicago author-date (all authors)
Vanpoucke, Griet, Steven Goossens, Bram De Craene, Barbara Gilbert, Frans Van Roy, and Geert Berx. 2004. “GATA-4 and MEF2C Transcription Factors Control the Tissue-specific Expression of the αT-catenin Gene CTNNA3.” Nucleic Acids Research 32 (14): 4155–4165.
Vancouver
1.
Vanpoucke G, Goossens S, De Craene B, Gilbert B, Van Roy F, Berx G. GATA-4 and MEF2C transcription factors control the tissue-specific expression of the αT-catenin gene CTNNA3. NUCLEIC ACIDS RESEARCH. 2004;32(14):4155–65.
IEEE
[1]
G. Vanpoucke, S. Goossens, B. De Craene, B. Gilbert, F. Van Roy, and G. Berx, “GATA-4 and MEF2C transcription factors control the tissue-specific expression of the αT-catenin gene CTNNA3,” NUCLEIC ACIDS RESEARCH, vol. 32, no. 14, pp. 4155–4165, 2004.
@article{296178,
  abstract     = {alphaT-catenin is a recently identified member of the alpha-catenin family of cell-cell adhesion molecules. Its expression is restricted mainly to cardiomyocytes, although it is also expressed in skeletal muscle, testis and brain. Like other alpha-catenins, alphaT-catenin provides an indispensable link between a cadherin-based adhesion complex and the actin cytoskeleton, resulting in strong cell-cell adhesion. We show here that the tissue-specificity of alphaT-catenin expression is controlled by its promoter region. By in silico, analysis, we found that the alphaT-catenin promoter contains several binding sites for cardiac and muscle-specific transcription factors. By co-transfection studies in P19 embryonal carcinoma cells, we demonstrated that MEF2C and GATA-4 each have an activating effect on the alphaT-catenin promoter. Transfections with wildtype and mutant promoter constructs in cardiac HL-1 cells indicated that one GATA box is absolutely required for high alphaT-catenin promoter activity in these cells. Furthermore, we showed that the GATA-4 transcription factor specifically binds and activates the alphaT-catenin promoter in vivo in cardiac HL-1 cells. In vivo promoter analysis in transgenic mice revealed that the isolated alphaT-catenin promoter region could direct the tissue-specific expression of a LacZ reporter gene in concordance with endogenous alphaT-catenin expression.},
  author       = {Vanpoucke, Griet and Goossens, Steven and De Craene, Bram and Gilbert, Barbara and Van Roy, Frans and Berx, Geert},
  issn         = {0305-1048},
  journal      = {NUCLEIC ACIDS RESEARCH},
  keywords     = {PROSTATE-CANCER CELLS,E-CADHERIN,ALZHEIMERS-DISEASE,HUMAN BRAIN,FACTOR 2C,PROTEIN,ADHESION,HEART,MORPHOGENESIS,PROMOTERS},
  language     = {eng},
  number       = {14},
  pages        = {4155--4165},
  title        = {GATA-4 and MEF2C transcription factors control the tissue-specific expression of the αT-catenin gene CTNNA3},
  url          = {http://dx.doi.org/10.1093/nar/gkh727},
  volume       = {32},
  year         = {2004},
}

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