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Effect of proinflammatory mediators and glucocorticoids on L-selectin expression in peripheral blood neutrophils from dairy cows in various stages of lactation

Araceli Diez Fraile, Evelyne Meyer UGent, Luc Duchateau UGent and Christian Burvenich UGent (2004) AMERICAN JOURNAL OF VETERINARY RESEARCH. 65(10). p.1421-1426
abstract
Objective-To determine whether proinflammatory mediators and glucocorticoids affect CD62L(L-selectin) expression on peripheral blood neutrophils from cows in various stages of lactation. Animals-100 healthy dairy cows during early (13.1 +/- 0.79 days after parturition; n = 31), peak (58.7 +/- 1.64 days after parturition; 31), and mid (1372 +/- 2.59 days after parturition; 38) lactation. Procedure-In vitro effects of relevant proinflammatory mediators that are released in response to mastitis caused by gram-negative bacteria such as Lipopolysaccharide (endotoxin), tumor necrosis factor-alpha, and platelet-activating factor (PAIF) on CD62L expression on bovine neutrophils were assessed by flow cytometry. Influences of cortisol and dexamethasone on CD62L expression on bovine neutrophils were also investigated. Results-Basal CD62L expression on neutrophils from cows during early, peak, and mid lactation were similar. Lipopolysaccharide and tumor necrosis factor-a had no effect on CD62L expression on neutrophils from cows at any stage of lactation. Conversely, PAF elicited a time- and dose-dependent, down regulatory effect on CD62L expression. However, no differential shedding of CD62L from neutrophils of cows at any stage of lactation were detected. In addition, no effects on CD62L expression on bovine neutrophils after whole blood incubation with cortisol or dexamethasone were observed. Incubation with glucocorticoids did not prevent the down regulatory effect of PAF on CD62L expression. Conclusions and Clinical Relevance-Comparable basal CD62L expression on bovine neutrophils and equal amounts of CD62L shedding from bovine neutrophils during all stages of lactation suggest that variations in CD62L density are not a likely cause of susceptibility of cows to coliform-induced mastitis during early lactation.
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author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ADHESION MOLECULES, IN-VITRO, POLYMORPHONUCLEAR LEUKOCYTES, COLIFORM MASTITIS, BOVINE NEUTROPHILS, PLATELET-ACTIVATING-FACTOR, NECROSIS-FACTOR-ALPHA, IMMUNE-SYSTEM, ESCHERICHIA-COLI, STAPHYLOCOCCUS-AUREUS
journal title
AMERICAN JOURNAL OF VETERINARY RESEARCH
Am. J. Vet. Res.
volume
65
issue
10
pages
1421-1426 pages
Web of Science type
Article
Web of Science id
000224069100015
JCR category
VETERINARY SCIENCES
JCR impact factor
1.125 (2004)
JCR rank
32/123 (2004)
JCR quartile
2 (2004)
ISSN
0002-9645
language
English
UGent publication?
yes
classification
A1
additional info
No full text
id
296003
handle
http://hdl.handle.net/1854/LU-296003
date created
2004-11-15 11:19:00
date last changed
2016-12-19 15:42:29
@article{296003,
  abstract     = {Objective-To determine whether proinflammatory mediators and glucocorticoids affect CD62L(L-selectin) expression on peripheral blood neutrophils from cows in various stages of lactation.
Animals-100 healthy dairy cows during early (13.1 +/- 0.79 days after parturition; n = 31), peak (58.7 +/- 1.64 days after parturition; 31), and mid (1372 +/- 2.59 days after parturition; 38) lactation.
Procedure-In vitro effects of relevant proinflammatory mediators that are released in response to mastitis caused by gram-negative bacteria such as Lipopolysaccharide (endotoxin), tumor necrosis factor-alpha, and platelet-activating factor (PAIF) on CD62L expression on bovine neutrophils were assessed by flow cytometry. Influences of cortisol and dexamethasone on CD62L expression on bovine neutrophils were also investigated.
Results-Basal CD62L expression on neutrophils from cows during early, peak, and mid lactation were similar. Lipopolysaccharide and tumor necrosis factor-a had no effect on CD62L expression on neutrophils from cows at any stage of lactation. Conversely, PAF elicited a time- and dose-dependent, down regulatory effect on CD62L expression. However, no differential shedding of CD62L from neutrophils of cows at any stage of lactation were detected. In addition, no effects on CD62L expression on bovine neutrophils after whole blood incubation with cortisol or dexamethasone were observed. Incubation with glucocorticoids did not prevent the down regulatory effect of PAF on CD62L expression.
Conclusions and Clinical Relevance-Comparable basal CD62L expression on bovine neutrophils and equal amounts of CD62L shedding from bovine neutrophils during all stages of lactation suggest that variations in CD62L density are not a likely cause of susceptibility of cows to coliform-induced mastitis during early lactation.},
  author       = {Diez Fraile, Araceli and Meyer, Evelyne and Duchateau, Luc and Burvenich, Christian},
  issn         = {0002-9645},
  journal      = {AMERICAN JOURNAL OF VETERINARY RESEARCH},
  keyword      = {ADHESION MOLECULES,IN-VITRO,POLYMORPHONUCLEAR LEUKOCYTES,COLIFORM MASTITIS,BOVINE NEUTROPHILS,PLATELET-ACTIVATING-FACTOR,NECROSIS-FACTOR-ALPHA,IMMUNE-SYSTEM,ESCHERICHIA-COLI,STAPHYLOCOCCUS-AUREUS},
  language     = {eng},
  number       = {10},
  pages        = {1421--1426},
  title        = {Effect of proinflammatory mediators and glucocorticoids on L-selectin expression in peripheral blood neutrophils from dairy cows in various stages of lactation},
  volume       = {65},
  year         = {2004},
}

Chicago
Diez Fraile, Araceli, Evelyne Meyer, Luc Duchateau, and Christian Burvenich. 2004. “Effect of Proinflammatory Mediators and Glucocorticoids on L-selectin Expression in Peripheral Blood Neutrophils from Dairy Cows in Various Stages of Lactation.” American Journal of Veterinary Research 65 (10): 1421–1426.
APA
Diez Fraile, A., Meyer, E., Duchateau, L., & Burvenich, C. (2004). Effect of proinflammatory mediators and glucocorticoids on L-selectin expression in peripheral blood neutrophils from dairy cows in various stages of lactation. AMERICAN JOURNAL OF VETERINARY RESEARCH, 65(10), 1421–1426.
Vancouver
1.
Diez Fraile A, Meyer E, Duchateau L, Burvenich C. Effect of proinflammatory mediators and glucocorticoids on L-selectin expression in peripheral blood neutrophils from dairy cows in various stages of lactation. AMERICAN JOURNAL OF VETERINARY RESEARCH. 2004;65(10):1421–6.
MLA
Diez Fraile, Araceli, Evelyne Meyer, Luc Duchateau, et al. “Effect of Proinflammatory Mediators and Glucocorticoids on L-selectin Expression in Peripheral Blood Neutrophils from Dairy Cows in Various Stages of Lactation.” AMERICAN JOURNAL OF VETERINARY RESEARCH 65.10 (2004): 1421–1426. Print.