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Increased expression of IL-19 in the epithelium of patients with chronic rhinosinusitis and nasal polyps

E Pace, V Scafidi, D Di Bona, L Siena, G Chiappara, M Ferraro, S La Grutta, S Gallina, R Speciale and A Ballacchino, et al. (2012) ALLERGY. 67(7). p.878-886
abstract
Background : Chronic rhinosinusitis (CRS) is an inflammation of the nose and of the paranasal sinuses. The involvement of the respiratory epithelium in the mechanisms of CRS is poorly understood. Aims : Among proteins expressed by nasal epithelial cells in CRS, IL-19 may have key functions. We here aimed to determine the expression and regulation of IL-19. Methods : Nasal biopsies from normal subjects (n = 12), subjects with CRS but without nasal polyps (NP) (CRSsNP, n = 12) and with CRS with NP (CRSwNP, n = 15) were collected. Human Asthma Gene Array and real-time PCR were used to evaluate gene expression, western blot analysis and immunohistochemistry for protein expression. Results for IL-19 were confirmed by real-time PCR. The constitutive and stimulated (LPS, TGF beta) expression of IL-19 and cell proliferation were evaluated in a nasal epithelial cell line (RPMI 2650). Results : Human Asthma Gene Array showed an increased IL-19 gene expression in NP from patients with CRS in comparison with normal subjects. Real-time PCR confirmed the IL-19 mRNA up-regulation in patients with CRSwNP and showed an up-regulation of IL-19, at lower extent, in patients with chronic rhinosinusitis without nasal polyps (CRSsNP) in comparison with normal subjects. Western blot analysis confirmed that IL-19 is increased also at protein level in patients with CRSwNP in comparison with normal subjects. In NP, IL-19 is highly expressed in the metaplastic nasal epithelium when compared to normal or hyperplastic epithelium. LPS stimulation increased IL-19 expression, and recombinant IL-19 increased cell proliferation in nasal epithelial cells. Conclusions : IL-19 is overexpressed in the epithelium in CRSwNP and increases epithelial cell proliferation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
asthma, inflammation, nasal polyps, rhinitis, SINUS DISEASE, POTENTIAL ROLE, ASTHMA, CELLS, INTERLEUKIN-19, CYTOKINES, IL-20, ACTIVATION, RESPONSES, RECEPTOR
journal title
ALLERGY
Allergy
volume
67
issue
7
pages
878 - 886
Web of Science type
Article
Web of Science id
000304986800005
JCR category
ALLERGY
JCR impact factor
5.883 (2012)
JCR rank
2/23 (2012)
JCR quartile
1 (2012)
ISSN
0105-4538
DOI
10.1111/j.1398-9995.2012.02842.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2959855
handle
http://hdl.handle.net/1854/LU-2959855
date created
2012-07-10 11:42:02
date last changed
2015-06-17 10:11:42
@article{2959855,
  abstract     = {Background : Chronic rhinosinusitis (CRS) is an inflammation of the nose and of the paranasal sinuses. The involvement of the respiratory epithelium in the mechanisms of CRS is poorly understood.
Aims : Among proteins expressed by nasal epithelial cells in CRS, IL-19 may have key functions. We here aimed to determine the expression and regulation of IL-19.
Methods : Nasal biopsies from normal subjects (n = 12), subjects with CRS but without nasal polyps (NP) (CRSsNP, n = 12) and with CRS with NP (CRSwNP, n = 15) were collected. Human Asthma Gene Array and real-time PCR were used to evaluate gene expression, western blot analysis and immunohistochemistry for protein expression. Results for IL-19 were confirmed by real-time PCR. The constitutive and stimulated (LPS, TGF beta) expression of IL-19 and cell proliferation were evaluated in a nasal epithelial cell line (RPMI 2650).
Results : Human Asthma Gene Array showed an increased IL-19 gene expression in NP from patients with CRS in comparison with normal subjects. Real-time PCR confirmed the IL-19 mRNA up-regulation in patients with CRSwNP and showed an up-regulation of IL-19, at lower extent, in patients with chronic rhinosinusitis without nasal polyps (CRSsNP) in comparison with normal subjects. Western blot analysis confirmed that IL-19 is increased also at protein level in patients with CRSwNP in comparison with normal subjects. In NP, IL-19 is highly expressed in the metaplastic nasal epithelium when compared to normal or hyperplastic epithelium. LPS stimulation increased IL-19 expression, and recombinant IL-19 increased cell proliferation in nasal epithelial cells.
Conclusions : IL-19 is overexpressed in the epithelium in CRSwNP and increases epithelial cell proliferation.},
  author       = {Pace, E and Scafidi, V and Di Bona, D and Siena, L and Chiappara, G and Ferraro, M and La Grutta, S and Gallina, S and Speciale, R and Ballacchino, A and Bachert, Claus and Bousquet, J and Gjomarkaj, M},
  issn         = {0105-4538},
  journal      = {ALLERGY},
  keyword      = {asthma,inflammation,nasal polyps,rhinitis,SINUS DISEASE,POTENTIAL ROLE,ASTHMA,CELLS,INTERLEUKIN-19,CYTOKINES,IL-20,ACTIVATION,RESPONSES,RECEPTOR},
  language     = {eng},
  number       = {7},
  pages        = {878--886},
  title        = {Increased expression of IL-19 in the epithelium of patients with chronic rhinosinusitis and nasal polyps},
  url          = {http://dx.doi.org/10.1111/j.1398-9995.2012.02842.x},
  volume       = {67},
  year         = {2012},
}

Chicago
Pace, E, V Scafidi, D Di Bona, L Siena, G Chiappara, M Ferraro, S La Grutta, et al. 2012. “Increased Expression of IL-19 in the Epithelium of Patients with Chronic Rhinosinusitis and Nasal Polyps.” Allergy 67 (7): 878–886.
APA
Pace, E., Scafidi, V., Di Bona, D., Siena, L., Chiappara, G., Ferraro, M., La Grutta, S., et al. (2012). Increased expression of IL-19 in the epithelium of patients with chronic rhinosinusitis and nasal polyps. ALLERGY, 67(7), 878–886.
Vancouver
1.
Pace E, Scafidi V, Di Bona D, Siena L, Chiappara G, Ferraro M, et al. Increased expression of IL-19 in the epithelium of patients with chronic rhinosinusitis and nasal polyps. ALLERGY. 2012;67(7):878–86.
MLA
Pace, E, V Scafidi, D Di Bona, et al. “Increased Expression of IL-19 in the Epithelium of Patients with Chronic Rhinosinusitis and Nasal Polyps.” ALLERGY 67.7 (2012): 878–886. Print.