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Biphenyl and benzoate metabolism in a genomic context: outlining genome-wide metabolic networks in Burkholderia xenovorans LB400

Vincent Denef, J Park, TV Tsoi, JM Rouillard, H Zhang, JA Wibbenmeyer, Willy Verstraete UGent, E Gulari, SA Hashsham and JM Tiedje (2004) APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 70(8). p.4961-4970
abstract
We designed and successfully implemented the use of in situ-synthesized 45-mer oligonucleotide DNA microarrays (XeoChips) for genome-wide expression profiling of Burkholderia xenovorans LB400, which is among the best aerobic polychlorinated biphenyl degraders known so far. We conducted differential gene expression profiling during exponential growth on succinate, benzoate, and biphenyl as sole carbon sources and investigated the transcriptome of early-stationary-phase cells grown on biphenyl. Based on these experiments, we outlined metabolic pathways and summarized other cellular functions in the organism relevant for biphenyl and benzoate degradation. All genes previously identified as being directly involved in biphenyl degradation were up-regulated when cells were grown on biphenyl compared to expression in succinate-grown cells. For benzoate degradation, however, genes for an aerobic coenzyme A activation pathway were upregulated in biphenyl-grown cells, while the pathway for benzoate degradation via hydroxylation was upregulated in benzoate-grown cells. The early-stationary-phase biphenyl-grown cells showed similar expression of biphenyl pathway genes, but a surprising up-regulation of C-1 metabolic pathway genes was observed. The microarray results were validated by quantitative reverse transcription PCR with a subset of genes of interest. The XeoChips showed a chip-to-chip variation of 13.9%, compared to the 21.6% variation for spotted oligonucleotide microarrays, which is less variation than that typically reported for PCR product microarrays.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PSEUDOMONAS-PSEUDOALCALIGENES KF707, SP STRAIN LB400, DEGRADE POLYCHLORINATED-BIPHENYLS, MICROARRAY ANALYSIS, PCB BIODEGRADATION, GENE-EXPRESSION, GROWTH-PHASE, BPH LOCUS, PATHWAY, OLIGONUCLEOTIDE
journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Appl. Environ. Microbiol.
volume
70
issue
8
pages
4961-4970 pages
Web of Science type
Article
Web of Science id
000223290100070
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.81 (2004)
JCR rank
16/133 (2004)
JCR quartile
1 (2004)
ISSN
0099-2240
DOI
10.1128/AEM.70.4961-4970.2004
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
295827
handle
http://hdl.handle.net/1854/LU-295827
date created
2004-11-05 15:21:00
date last changed
2016-12-19 15:42:31
@article{295827,
  abstract     = {We designed and successfully implemented the use of in situ-synthesized 45-mer oligonucleotide DNA microarrays (XeoChips) for genome-wide expression profiling of Burkholderia xenovorans LB400, which is among the best aerobic polychlorinated biphenyl degraders known so far. We conducted differential gene expression profiling during exponential growth on succinate, benzoate, and biphenyl as sole carbon sources and investigated the transcriptome of early-stationary-phase cells grown on biphenyl. Based on these experiments, we outlined metabolic pathways and summarized other cellular functions in the organism relevant for biphenyl and benzoate degradation. All genes previously identified as being directly involved in biphenyl degradation were up-regulated when cells were grown on biphenyl compared to expression in succinate-grown cells. For benzoate degradation, however, genes for an aerobic coenzyme A activation pathway were upregulated in biphenyl-grown cells, while the pathway for benzoate degradation via hydroxylation was upregulated in benzoate-grown cells. The early-stationary-phase biphenyl-grown cells showed similar expression of biphenyl pathway genes, but a surprising up-regulation of C-1 metabolic pathway genes was observed. The microarray results were validated by quantitative reverse transcription PCR with a subset of genes of interest. The XeoChips showed a chip-to-chip variation of 13.9\%, compared to the 21.6\% variation for spotted oligonucleotide microarrays, which is less variation than that typically reported for PCR product microarrays.},
  author       = {Denef, Vincent and Park, J and Tsoi, TV and Rouillard, JM and Zhang, H and Wibbenmeyer, JA and Verstraete, Willy and Gulari, E and Hashsham, SA and Tiedje, JM},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {PSEUDOMONAS-PSEUDOALCALIGENES KF707,SP STRAIN LB400,DEGRADE POLYCHLORINATED-BIPHENYLS,MICROARRAY ANALYSIS,PCB BIODEGRADATION,GENE-EXPRESSION,GROWTH-PHASE,BPH LOCUS,PATHWAY,OLIGONUCLEOTIDE},
  language     = {eng},
  number       = {8},
  pages        = {4961--4970},
  title        = {Biphenyl and benzoate metabolism in a genomic context: outlining genome-wide metabolic networks in Burkholderia xenovorans LB400},
  url          = {http://dx.doi.org/10.1128/AEM.70.4961-4970.2004},
  volume       = {70},
  year         = {2004},
}

Chicago
Denef, Vincent, J Park, TV Tsoi, JM Rouillard, H Zhang, JA Wibbenmeyer, Willy Verstraete, E Gulari, SA Hashsham, and JM Tiedje. 2004. “Biphenyl and Benzoate Metabolism in a Genomic Context: Outlining Genome-wide Metabolic Networks in Burkholderia Xenovorans LB400.” Applied and Environmental Microbiology 70 (8): 4961–4970.
APA
Denef, V., Park, J., Tsoi, T., Rouillard, J., Zhang, H., Wibbenmeyer, J., Verstraete, W., et al. (2004). Biphenyl and benzoate metabolism in a genomic context: outlining genome-wide metabolic networks in Burkholderia xenovorans LB400. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(8), 4961–4970.
Vancouver
1.
Denef V, Park J, Tsoi T, Rouillard J, Zhang H, Wibbenmeyer J, et al. Biphenyl and benzoate metabolism in a genomic context: outlining genome-wide metabolic networks in Burkholderia xenovorans LB400. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2004;70(8):4961–70.
MLA
Denef, Vincent, J Park, TV Tsoi, et al. “Biphenyl and Benzoate Metabolism in a Genomic Context: Outlining Genome-wide Metabolic Networks in Burkholderia Xenovorans LB400.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 70.8 (2004): 4961–4970. Print.