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Influence of 17 beta-estradiol, progesterone, and dexamethasone on diapedesis and viability of bovine blood polymorphonuclear leukocytes

(2004) JOURNAL OF DAIRY SCIENCE. 87(10). p.3340-3349
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Organization
Abstract
The aim of the current study was to investigate whether polymorphonuclear leukocyte (PMN) diapedesis and viability are influenced by steroid hormones. Using an in vitro model with different types of cell layers ( bovine mammary epithelial cells and fibroblasts), we investigate whether steroid hormone treatments (17beta-estradiol, progesterone, and dexamethasone) have an influence on the diapedesis capacity and viability of PMN. In addition, we studied apoptosis of PMN in the in vitro model and evaluated the influence of different types of cell layers and steroid hormone treatments on this process. A significant decrease in the number of viable PMN in the lower compartment of the in vitro model (i.e., number of migrated PMN x viability after migration) was found after 17beta-estradiol treatment, whereas no influence was detected after progesterone or dexamethasone treatment. The effect of 17beta-estradiol was not due to a lower viability before migration as none of the treatments caused a significant effect on the viability before diapedesis. This treatment effect was not influenced by endogenous 17beta-estradiol or progesterone levels before isolation because there was no correlation between these plasma levels and PMN diapedesis capacity or viability. Furthermore, migration through epithelial cells caused a significant decrease in viability of PMN due to increased apoptosis but not necrosis.
Keywords
diapedesis, steroid, viability, polymorphonuclear leukocyte, SEX STEROID-HORMONES, HUMAN NEUTROPHILS, MAMMARY-GLAND, ESCHERICHIA-COLI, BURST ACTIVITY, IN-VITRO, APOPTOSIS, MILK, GLUCOCORTICOIDS, MASTITIS

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MLA
Lamote, Inge et al. “Influence of 17 Beta-estradiol, Progesterone, and Dexamethasone on Diapedesis and Viability of Bovine Blood Polymorphonuclear Leukocytes.” JOURNAL OF DAIRY SCIENCE 87.10 (2004): 3340–3349. Print.
APA
Lamote, Inge, Meyer, E., Duchateau, L., & Burvenich, C. (2004). Influence of 17 beta-estradiol, progesterone, and dexamethasone on diapedesis and viability of bovine blood polymorphonuclear leukocytes. JOURNAL OF DAIRY SCIENCE, 87(10), 3340–3349.
Chicago author-date
Lamote, Inge, Evelyne Meyer, Luc Duchateau, and Christian Burvenich. 2004. “Influence of 17 Beta-estradiol, Progesterone, and Dexamethasone on Diapedesis and Viability of Bovine Blood Polymorphonuclear Leukocytes.” Journal of Dairy Science 87 (10): 3340–3349.
Chicago author-date (all authors)
Lamote, Inge, Evelyne Meyer, Luc Duchateau, and Christian Burvenich. 2004. “Influence of 17 Beta-estradiol, Progesterone, and Dexamethasone on Diapedesis and Viability of Bovine Blood Polymorphonuclear Leukocytes.” Journal of Dairy Science 87 (10): 3340–3349.
Vancouver
1.
Lamote I, Meyer E, Duchateau L, Burvenich C. Influence of 17 beta-estradiol, progesterone, and dexamethasone on diapedesis and viability of bovine blood polymorphonuclear leukocytes. JOURNAL OF DAIRY SCIENCE. AMER DAIRY SCIENCE ASSOC; 2004;87(10):3340–9.
IEEE
[1]
I. Lamote, E. Meyer, L. Duchateau, and C. Burvenich, “Influence of 17 beta-estradiol, progesterone, and dexamethasone on diapedesis and viability of bovine blood polymorphonuclear leukocytes,” JOURNAL OF DAIRY SCIENCE, vol. 87, no. 10, pp. 3340–3349, 2004.
@article{295420,
  abstract     = {The aim of the current study was to investigate whether polymorphonuclear leukocyte (PMN) diapedesis and viability are influenced by steroid hormones. Using an in vitro model with different types of cell layers ( bovine mammary epithelial cells and fibroblasts), we investigate whether steroid hormone treatments (17beta-estradiol, progesterone, and dexamethasone) have an influence on the diapedesis capacity and viability of PMN. In addition, we studied apoptosis of PMN in the in vitro model and evaluated the influence of different types of cell layers and steroid hormone treatments on this process. A significant decrease in the number of viable PMN in the lower compartment of the in vitro model (i.e., number of migrated PMN x viability after migration) was found after 17beta-estradiol treatment, whereas no influence was detected after progesterone or dexamethasone treatment. The effect of 17beta-estradiol was not due to a lower viability before migration as none of the treatments caused a significant effect on the viability before diapedesis. This treatment effect was not influenced by endogenous 17beta-estradiol or progesterone levels before isolation because there was no correlation between these plasma levels and PMN diapedesis capacity or viability. Furthermore, migration through epithelial cells caused a significant decrease in viability of PMN due to increased apoptosis but not necrosis.},
  author       = {Lamote, Inge and Meyer, Evelyne and Duchateau, Luc and Burvenich, Christian},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keywords     = {diapedesis,steroid,viability,polymorphonuclear leukocyte,SEX STEROID-HORMONES,HUMAN NEUTROPHILS,MAMMARY-GLAND,ESCHERICHIA-COLI,BURST ACTIVITY,IN-VITRO,APOPTOSIS,MILK,GLUCOCORTICOIDS,MASTITIS},
  language     = {eng},
  number       = {10},
  pages        = {3340--3349},
  publisher    = {AMER DAIRY SCIENCE ASSOC},
  title        = {Influence of 17 beta-estradiol, progesterone, and dexamethasone on diapedesis and viability of bovine blood polymorphonuclear leukocytes},
  volume       = {87},
  year         = {2004},
}

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