Ghent University Academic Bibliography

Advanced

Influence of 17 beta-estradiol, progesterone, and dexamethasone on diapedesis and viability of bovine blood polymorphonuclear leukocytes

Inge Lamote, Evelyne Meyer UGent, Luc Duchateau UGent and Christian Burvenich UGent (2004) JOURNAL OF DAIRY SCIENCE. 87(10). p.3340-3349
abstract
The aim of the current study was to investigate whether polymorphonuclear leukocyte (PMN) diapedesis and viability are influenced by steroid hormones. Using an in vitro model with different types of cell layers ( bovine mammary epithelial cells and fibroblasts), we investigate whether steroid hormone treatments (17beta-estradiol, progesterone, and dexamethasone) have an influence on the diapedesis capacity and viability of PMN. In addition, we studied apoptosis of PMN in the in vitro model and evaluated the influence of different types of cell layers and steroid hormone treatments on this process. A significant decrease in the number of viable PMN in the lower compartment of the in vitro model (i.e., number of migrated PMN x viability after migration) was found after 17beta-estradiol treatment, whereas no influence was detected after progesterone or dexamethasone treatment. The effect of 17beta-estradiol was not due to a lower viability before migration as none of the treatments caused a significant effect on the viability before diapedesis. This treatment effect was not influenced by endogenous 17beta-estradiol or progesterone levels before isolation because there was no correlation between these plasma levels and PMN diapedesis capacity or viability. Furthermore, migration through epithelial cells caused a significant decrease in viability of PMN due to increased apoptosis but not necrosis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
diapedesis, steroid, viability, polymorphonuclear leukocyte, SEX STEROID-HORMONES, HUMAN NEUTROPHILS, MAMMARY-GLAND, ESCHERICHIA-COLI, BURST ACTIVITY, IN-VITRO, APOPTOSIS, MILK, GLUCOCORTICOIDS, MASTITIS
journal title
JOURNAL OF DAIRY SCIENCE
J. Dairy Sci.
volume
87
issue
10
pages
3340-3349 pages
publisher
AMER DAIRY SCIENCE ASSOC
Web of Science type
Article
Web of Science id
000224011800025
JCR category
AGRICULTURE, DAIRY & ANIMAL SCIENCE
JCR impact factor
2.134 (2004)
JCR rank
3/41 (2004)
JCR quartile
1 (2004)
ISSN
0022-0302
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
295420
handle
http://hdl.handle.net/1854/LU-295420
date created
2004-10-25 08:42:00
date last changed
2010-06-23 11:48:09
@article{295420,
  abstract     = {The aim of the current study was to investigate whether polymorphonuclear leukocyte (PMN) diapedesis and viability are influenced by steroid hormones. Using an in vitro model with different types of cell layers ( bovine mammary epithelial cells and fibroblasts), we investigate whether steroid hormone treatments (17beta-estradiol, progesterone, and dexamethasone) have an influence on the diapedesis capacity and viability of PMN. In addition, we studied apoptosis of PMN in the in vitro model and evaluated the influence of different types of cell layers and steroid hormone treatments on this process. A significant decrease in the number of viable PMN in the lower compartment of the in vitro model (i.e., number of migrated PMN x viability after migration) was found after 17beta-estradiol treatment, whereas no influence was detected after progesterone or dexamethasone treatment. The effect of 17beta-estradiol was not due to a lower viability before migration as none of the treatments caused a significant effect on the viability before diapedesis. This treatment effect was not influenced by endogenous 17beta-estradiol or progesterone levels before isolation because there was no correlation between these plasma levels and PMN diapedesis capacity or viability. Furthermore, migration through epithelial cells caused a significant decrease in viability of PMN due to increased apoptosis but not necrosis.},
  author       = {Lamote, Inge and Meyer, Evelyne and Duchateau, Luc and Burvenich, Christian},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keyword      = {diapedesis,steroid,viability,polymorphonuclear leukocyte,SEX STEROID-HORMONES,HUMAN NEUTROPHILS,MAMMARY-GLAND,ESCHERICHIA-COLI,BURST ACTIVITY,IN-VITRO,APOPTOSIS,MILK,GLUCOCORTICOIDS,MASTITIS},
  language     = {eng},
  number       = {10},
  pages        = {3340--3349},
  publisher    = {AMER DAIRY SCIENCE ASSOC},
  title        = {Influence of 17 beta-estradiol, progesterone, and dexamethasone on diapedesis and viability of bovine blood polymorphonuclear leukocytes},
  volume       = {87},
  year         = {2004},
}

Chicago
Lamote, Inge, Evelyne Meyer, Luc Duchateau, and Christian Burvenich. 2004. “Influence of 17 Beta-estradiol, Progesterone, and Dexamethasone on Diapedesis and Viability of Bovine Blood Polymorphonuclear Leukocytes.” Journal of Dairy Science 87 (10): 3340–3349.
APA
Lamote, I., Meyer, E., Duchateau, L., & Burvenich, C. (2004). Influence of 17 beta-estradiol, progesterone, and dexamethasone on diapedesis and viability of bovine blood polymorphonuclear leukocytes. JOURNAL OF DAIRY SCIENCE, 87(10), 3340–3349.
Vancouver
1.
Lamote I, Meyer E, Duchateau L, Burvenich C. Influence of 17 beta-estradiol, progesterone, and dexamethasone on diapedesis and viability of bovine blood polymorphonuclear leukocytes. JOURNAL OF DAIRY SCIENCE. AMER DAIRY SCIENCE ASSOC; 2004;87(10):3340–9.
MLA
Lamote, Inge, Evelyne Meyer, Luc Duchateau, et al. “Influence of 17 Beta-estradiol, Progesterone, and Dexamethasone on Diapedesis and Viability of Bovine Blood Polymorphonuclear Leukocytes.” JOURNAL OF DAIRY SCIENCE 87.10 (2004): 3340–3349. Print.