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Comparison of L-selectin and Mac-1 expression on blood and milk neutrophils during experimental Escherichia coli-induced mastitis in cows

A DIEZ-FRAILLE, J MEHRZAD, Evelyne Meyer UGent, Luc Duchateau UGent and Christian Burvenich UGent (2004) AMERICAN JOURNAL OF VETERINARY RESEARCH. 65(8). p.1164-1171
abstract
Objective-To evaluate L-selectin (CD62L) and Mac-1 (CD11b) expression at the surface of blood and milk neutrophils during the early inflammatory response to Escherichia coli-induced mastitis in cows. Animals-6 healthy Holstein heifers in early lactation. Procedure-Blood and milk samples were collected before and after intramammary administration of 10(4) CFU's of E coli in the left mammary gland quarters. Bacterial counts and electrolyte concentrations in milk, rectal temperature, differential blood leukocyte counts, milk somatic cell counts, neutrophil viability, and the expression of CD62L and CD11b on blood and milk neutrophils were determined longitudinally. Results-Bacteria grew during the first 6 hours after inoculation with a pronounced leukocytic influx. Coincident with neutrophil influx was an increase in CD62L(+) and CD11b(+) milk neutrophils, as well as an improved viability of milk neutrophils. The peak of the inflammatory reaction was reached approximately 12 hours after E coli inoculation. From that time forward, changes in CD62L and CD11b expression were opposed to each other, with a decrease in CD62L expression and an increase in CD11b expression on blood and milk neutrophils; the magnitude of the differences in CD62L and CD11b expression between blood and milk neutrophils decreased. Percentages of CD62L(+) and CD11b(+) milk neutrophils increased to percentages that were similar to blood neutrophils (ie, approx 92%). Conclusions and Clinical Relevance-The presence of adhesion molecules on a large percentage of milk neutrophils during the acute inflammatory response, together with the changes in receptor density, suggest a major role for CD62L and CD11b in neutrophil function during coliform mastitis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
STAPHYLOCOCCUS-AUREUS, LEUKOCYTES, INCREASED SURFACE EXPRESSION, ADHESION MOLECULES, MAMMARY-GLAND, DAIRY-COWS, LIPOTEICHOIC ACID, MONOCLONAL-ANTIBODIES, CD11B/CD18, UP-REGULATION
journal title
AMERICAN JOURNAL OF VETERINARY RESEARCH
Am. J. Vet. Res.
volume
65
issue
8
pages
1164-1171 pages
publisher
AMER VETERINARY MEDICAL ASSOC
Web of Science type
Article
Web of Science id
000222910500019
JCR category
VETERINARY SCIENCES
JCR impact factor
1.125 (2004)
JCR rank
32/123 (2004)
JCR quartile
2 (2004)
ISSN
0002-9645
language
English
UGent publication?
yes
classification
A1
additional info
No full text
id
295093
handle
http://hdl.handle.net/1854/LU-295093
date created
2004-10-04 10:11:00
date last changed
2016-12-19 15:42:29
@article{295093,
  abstract     = {Objective-To evaluate L-selectin (CD62L) and Mac-1 (CD11b) expression at the surface of blood and milk neutrophils during the early inflammatory response to Escherichia coli-induced mastitis in cows.
Animals-6 healthy Holstein heifers in early lactation.
Procedure-Blood and milk samples were collected before and after intramammary administration of 10(4) CFU's of E coli in the left mammary gland quarters. Bacterial counts and electrolyte concentrations in milk, rectal temperature, differential blood leukocyte counts, milk somatic cell counts, neutrophil viability, and the expression of CD62L and CD11b on blood and milk neutrophils were determined longitudinally.
Results-Bacteria grew during the first 6 hours after inoculation with a pronounced leukocytic influx. Coincident with neutrophil influx was an increase in CD62L(+) and CD11b(+) milk neutrophils, as well as an improved viability of milk neutrophils. The peak of the inflammatory reaction was reached approximately 12 hours after E coli inoculation. From that time forward, changes in CD62L and CD11b expression were opposed to each other, with a decrease in CD62L expression and an increase in CD11b expression on blood and milk neutrophils; the magnitude of the differences in CD62L and CD11b expression between blood and milk neutrophils decreased. Percentages of CD62L(+) and CD11b(+) milk neutrophils increased to percentages that were similar to blood neutrophils (ie, approx 92\%).
Conclusions and Clinical Relevance-The presence of adhesion molecules on a large percentage of milk neutrophils during the acute inflammatory response, together with the changes in receptor density, suggest a major role for CD62L and CD11b in neutrophil function during coliform mastitis.},
  author       = {DIEZ-FRAILLE, A and MEHRZAD, J and Meyer, Evelyne and Duchateau, Luc and Burvenich, Christian},
  issn         = {0002-9645},
  journal      = {AMERICAN JOURNAL OF VETERINARY RESEARCH},
  keyword      = {STAPHYLOCOCCUS-AUREUS,LEUKOCYTES,INCREASED SURFACE EXPRESSION,ADHESION MOLECULES,MAMMARY-GLAND,DAIRY-COWS,LIPOTEICHOIC ACID,MONOCLONAL-ANTIBODIES,CD11B/CD18,UP-REGULATION},
  language     = {eng},
  number       = {8},
  pages        = {1164--1171},
  publisher    = {AMER VETERINARY MEDICAL ASSOC},
  title        = {Comparison of L-selectin and Mac-1 expression on blood and milk neutrophils during experimental Escherichia coli-induced mastitis in cows},
  volume       = {65},
  year         = {2004},
}

Chicago
DIEZ-FRAILLE, A, J MEHRZAD, Evelyne Meyer, Luc Duchateau, and Christian Burvenich. 2004. “Comparison of L-selectin and Mac-1 Expression on Blood and Milk Neutrophils During Experimental Escherichia Coli-induced Mastitis in Cows.” American Journal of Veterinary Research 65 (8): 1164–1171.
APA
DIEZ-FRAILLE, A., MEHRZAD, J., Meyer, E., Duchateau, L., & Burvenich, C. (2004). Comparison of L-selectin and Mac-1 expression on blood and milk neutrophils during experimental Escherichia coli-induced mastitis in cows. AMERICAN JOURNAL OF VETERINARY RESEARCH, 65(8), 1164–1171.
Vancouver
1.
DIEZ-FRAILLE A, MEHRZAD J, Meyer E, Duchateau L, Burvenich C. Comparison of L-selectin and Mac-1 expression on blood and milk neutrophils during experimental Escherichia coli-induced mastitis in cows. AMERICAN JOURNAL OF VETERINARY RESEARCH. AMER VETERINARY MEDICAL ASSOC; 2004;65(8):1164–71.
MLA
DIEZ-FRAILLE, A, J MEHRZAD, Evelyne Meyer, et al. “Comparison of L-selectin and Mac-1 Expression on Blood and Milk Neutrophils During Experimental Escherichia Coli-induced Mastitis in Cows.” AMERICAN JOURNAL OF VETERINARY RESEARCH 65.8 (2004): 1164–1171. Print.