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In vitro regulation of Mac-1 expression on bovine polymorphonuclear leukocytes by endotoxin and tumor necrosis factor-alpha at different stages of lactation

A DIEZ-FRAILE, Evelyne Meyer (UGent) , Luc Duchateau (UGent) , Max Paape (UGent) and Christian Burvenich (UGent)
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Abstract
The purpose of this in vitro study is to clarify some of the underlying mechanisms leading to the decreased migratory capacity of polymorphonuclear leukocytes (PMN) during mastitis in dairy cows soon after calving. Surface expression of Mac-1 (CD11b, CR3) on PMN and of CD14 on monocytes was measured in early- (EL), peak- (PL), and midlactation (ML) by flow cytometric analysis. In addition, we evaluated the effect of lipopolysaccharide (LPS) and tumor necrosis factor (TNF)-alpha on CD11b surface expression in PMN at different stages of lactation in a whole blood model. During EL, while resting monocytes expressed diminished levels of CD14, the basal expression of CD11b on PMN was not significantly altered. The relative increase of CD11b on PMN after incubation with LPS or TNF-alpha did not significantly differ among EL, PL, or ML at any of the concentrations tested. The current findings do not support an important role for basal CD11b levels nor for a defective mobilization of CD11b by LPS and TNF-alpha in the reduced migratory capacity of PMN during EL.
Keywords
ESCHERICHIA-COLI, PERIPARTURIENT PERIOD, NEUTROPHILS, ADHESION, RECEPTOR, COWS, BLOOD, MILK, COLIFORM MASTITIS, L-SELECTIN

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Chicago
DIEZ-FRAILE, A, Evelyne Meyer, Luc Duchateau, Max Paape, and Christian Burvenich. 2004. “In Vitro Regulation of Mac-1 Expression on Bovine Polymorphonuclear Leukocytes by Endotoxin and Tumor Necrosis Factor-alpha at Different Stages of Lactation.” Canadian Journal of Veterinary Research-revue Canadienne De Recherche Veterinaire 68 (3): 232–235.
APA
DIEZ-FRAILE, A., Meyer, E., Duchateau, L., Paape, M., & Burvenich, C. (2004). In vitro regulation of Mac-1 expression on bovine polymorphonuclear leukocytes by endotoxin and tumor necrosis factor-alpha at different stages of lactation. CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE, 68(3), 232–235.
Vancouver
1.
DIEZ-FRAILE A, Meyer E, Duchateau L, Paape M, Burvenich C. In vitro regulation of Mac-1 expression on bovine polymorphonuclear leukocytes by endotoxin and tumor necrosis factor-alpha at different stages of lactation. CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE. CANADIAN VET MED ASSOC; 2004;68(3):232–5.
MLA
DIEZ-FRAILE, A, Evelyne Meyer, Luc Duchateau, et al. “In Vitro Regulation of Mac-1 Expression on Bovine Polymorphonuclear Leukocytes by Endotoxin and Tumor Necrosis Factor-alpha at Different Stages of Lactation.” CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE 68.3 (2004): 232–235. Print.
@article{295091,
  abstract     = {The purpose of this in vitro study is to clarify some of the underlying mechanisms leading to the decreased migratory capacity of polymorphonuclear leukocytes (PMN) during mastitis in dairy cows soon after calving. Surface expression of Mac-1 (CD11b, CR3) on PMN and of CD14 on monocytes was measured in early- (EL), peak- (PL), and midlactation (ML) by flow cytometric analysis. In addition, we evaluated the effect of lipopolysaccharide (LPS) and tumor necrosis factor (TNF)-alpha on CD11b surface expression in PMN at different stages of lactation in a whole blood model. During EL, while resting monocytes expressed diminished levels of CD14, the basal expression of CD11b on PMN was not significantly altered. The relative increase of CD11b on PMN after incubation with LPS or TNF-alpha did not significantly differ among EL, PL, or ML at any of the concentrations tested. The current findings do not support an important role for basal CD11b levels nor for a defective mobilization of CD11b by LPS and TNF-alpha in the reduced migratory capacity of PMN during EL.},
  author       = {DIEZ-FRAILE, A and Meyer, Evelyne and Duchateau, Luc and Paape, Max and Burvenich, Christian},
  issn         = {0830-9000},
  journal      = {CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE},
  keyword      = {ESCHERICHIA-COLI,PERIPARTURIENT PERIOD,NEUTROPHILS,ADHESION,RECEPTOR,COWS,BLOOD,MILK,COLIFORM MASTITIS,L-SELECTIN},
  language     = {eng},
  number       = {3},
  pages        = {232--235},
  publisher    = {CANADIAN VET MED ASSOC},
  title        = {In vitro regulation of Mac-1 expression on bovine polymorphonuclear leukocytes by endotoxin and tumor necrosis factor-alpha at different stages of lactation},
  volume       = {68},
  year         = {2004},
}

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