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Breaking the species barrier: derivation of germline-competent embryonic stem cells from Mus spretus x C57BL/6 hybrids

(2004) STEM CELLS. 22(4). p.441-447
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Abstract
Embryonic stem (ES) cells, which can differentiate into almost all types of cells, have been derived from the house mouse Mus musculus, rat, rabbit, humans, and other species. Transmission of the genotype to the offspring of chimeras has been achieved only with M. musculus ES cells. limiting targeted mutagenesis using ES cells to this species. Mus spretus, which exhibits many genetic polymorphisms with M. musculus, displays dominant resistance to cancer and inflammation, making derived inbred strains very useful in positional cloning and interspecies mapping. We show here for the first time the derivation of ES cells from hybrid blastocysts, obtained by the mating of two different species, namely Mus muscalus and Mus spretus, and their use for the generation of chimeric mice that transmit the Mus spretus genotype and phenotype to the offspring. These hybrid ES cells allow the genetic manipulation of Mus spretus, as an alternative to Mus musculus.
Keywords
TNF, embryonic stem cell, Mus spretus, germline transmission, PLURIPOTENTIAL CELLS, IN-VITRO, MOUSE, MICE, LINES, ESTABLISHMENT, RESISTANCE, STERILITY, EVOLUTION, STRAINS

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Citation

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Chicago
Hochepied, Tino, Luc Schoonjans, Jan Staelens, Veerle Kreemers, Sophie Danloy, Leen Puimège, Désiré Collen, Frans Van Roy, and Claude Libert. 2004. “Breaking the Species Barrier: Derivation of Germline-competent Embryonic Stem Cells from Mus Spretus x C57BL/6 Hybrids.” Stem Cells 22 (4): 441–447.
APA
Hochepied, T., Schoonjans, L., Staelens, J., Kreemers, V., Danloy, S., Puimège, L., Collen, D., et al. (2004). Breaking the species barrier: derivation of germline-competent embryonic stem cells from Mus spretus x C57BL/6 hybrids. STEM CELLS, 22(4), 441–447.
Vancouver
1.
Hochepied T, Schoonjans L, Staelens J, Kreemers V, Danloy S, Puimège L, et al. Breaking the species barrier: derivation of germline-competent embryonic stem cells from Mus spretus x C57BL/6 hybrids. STEM CELLS. 2004;22(4):441–7.
MLA
Hochepied, Tino, Luc Schoonjans, Jan Staelens, et al. “Breaking the Species Barrier: Derivation of Germline-competent Embryonic Stem Cells from Mus Spretus x C57BL/6 Hybrids.” STEM CELLS 22.4 (2004): 441–447. Print.
@article{294857,
  abstract     = {Embryonic stem (ES) cells, which can differentiate into almost all types of cells, have been derived from the house mouse Mus musculus, rat, rabbit, humans, and other species. Transmission of the genotype to the offspring of chimeras has been achieved only with M. musculus ES cells. limiting targeted mutagenesis using ES cells to this species. Mus spretus, which exhibits many genetic polymorphisms with M. musculus, displays dominant resistance to cancer and inflammation, making derived inbred strains very useful in positional cloning and interspecies mapping. We show here for the first time the derivation of ES cells from hybrid blastocysts, obtained by the mating of two different species, namely Mus muscalus and Mus spretus, and their use for the generation of chimeric mice that transmit the Mus spretus genotype and phenotype to the offspring. These hybrid ES cells allow the genetic manipulation of Mus spretus, as an alternative to Mus musculus.},
  author       = {Hochepied, Tino and Schoonjans, Luc and Staelens, Jan and Kreemers, Veerle and Danloy, Sophie and Puim{\`e}ge, Leen and Collen, D{\'e}sir{\'e} and Van Roy, Frans and Libert, Claude},
  issn         = {1066-5099},
  journal      = {STEM CELLS},
  keyword      = {TNF,embryonic stem cell,Mus spretus,germline transmission,PLURIPOTENTIAL CELLS,IN-VITRO,MOUSE,MICE,LINES,ESTABLISHMENT,RESISTANCE,STERILITY,EVOLUTION,STRAINS},
  language     = {eng},
  number       = {4},
  pages        = {441--447},
  title        = {Breaking the species barrier: derivation of germline-competent embryonic stem cells from Mus spretus x C57BL/6 hybrids},
  url          = {http://dx.doi.org/10.1634/stemcells.22-4-441},
  volume       = {22},
  year         = {2004},
}

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