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Plakophilin-3 is required for late embryonic amphibian development, exhibiting roles in ectodermal and neural tissues

William A Munoz, Malgorzata Kloc, Kyucheol Cho, Moonsup Lee, Ilse Hofmann, Amy Sater, Kris Vleminckx UGent and Pierre D McCrea (2012) PLOS ONE. 7(4).
abstract
The p120-catenin family has undergone a significant expansion during the evolution of vertebrates, resulting in varied functions that have yet to be discerned or fully characterized. Likewise, members of the plakophilins, a related catenin subfamily, are found throughout the cell with little known about their functions outside the desmosomal plaque. While the plakophilin-3 (Pkp3) knockout mouse resulted in skin defects, we find larger, including lethal effects following its depletion in Xenopus. Pkp3, unlike some other characterized catenins in amphibians, does not have significant maternal deposits of mRNA. However, during embryogenesis, two Pkp3 protein products whose temporal expression is partially complimentary become expressed. Only the smaller of these products is found in adult Xenopus tissues, with an expression pattern exhibiting distinctions as well as overlaps with those observed in mammalian studies. We determined that Xenopus Pkp3 depletion causes a skin fragility phenotype in keeping with the mouse knockout, but more novel, Xenopus tailbud embryos are hyposensitive to touch even in embryos lacking outward discernable phenotypes, and we additionally resolved disruptions in certain peripheral neural structures, altered establishment and migration of neural crest, and defects in ectodermal multiciliated cells. The use of two distinct morpholinos, as well as rescue approaches, indicated the specificity of these effects. Our results point to the requirement of Pkp3 in amphibian embryogenesis, with functional roles in a number of tissue types.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DESMOSOMAL PLAQUE, CELL-ADHESION, RHO-FAMILY GTPASES, P120 CATENIN, BETA-CATENIN, TRANSCRIPTION FACTOR, EPITHELIAL-CELLS, PROTEIN PLAKOPHILIN-2, ACTIN CYTOSKELETON, FRAGILITY-SYNDROME
journal title
PLOS ONE
PLoS One
volume
7
issue
4
article_number
e34342
pages
14 pages
Web of Science type
Article
Web of Science id
000305010500006
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.73 (2012)
JCR rank
7/56 (2012)
JCR quartile
1 (2012)
ISSN
1932-6203
DOI
10.1371/journal.pone.0034342
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
2941018
handle
http://hdl.handle.net/1854/LU-2941018
date created
2012-06-28 11:06:22
date last changed
2012-07-09 16:51:54
@article{2941018,
  abstract     = {The p120-catenin family has undergone a significant expansion during the evolution of vertebrates, resulting in varied functions that have yet to be discerned or fully characterized. Likewise, members of the plakophilins, a related catenin subfamily, are found throughout the cell with little known about their functions outside the desmosomal plaque. While the plakophilin-3 (Pkp3) knockout mouse resulted in skin defects, we find larger, including lethal effects following its depletion in Xenopus. Pkp3, unlike some other characterized catenins in amphibians, does not have significant maternal deposits of mRNA. However, during embryogenesis, two Pkp3 protein products whose temporal expression is partially complimentary become expressed. Only the smaller of these products is found in adult Xenopus tissues, with an expression pattern exhibiting distinctions as well as overlaps with those observed in mammalian studies. We determined that Xenopus Pkp3 depletion causes a skin fragility phenotype in keeping with the mouse knockout, but more novel, Xenopus tailbud embryos are hyposensitive to touch even in embryos lacking outward discernable phenotypes, and we additionally resolved disruptions in certain peripheral neural structures, altered establishment and migration of neural crest, and defects in ectodermal multiciliated cells. The use of two distinct morpholinos, as well as rescue approaches, indicated the specificity of these effects. Our results point to the requirement of Pkp3 in amphibian embryogenesis, with functional roles in a number of tissue types.},
  articleno    = {e34342},
  author       = {Munoz, William A and Kloc, Malgorzata and Cho, Kyucheol and Lee, Moonsup and Hofmann, Ilse and Sater, Amy and Vleminckx, Kris and McCrea, Pierre D},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {DESMOSOMAL PLAQUE,CELL-ADHESION,RHO-FAMILY GTPASES,P120 CATENIN,BETA-CATENIN,TRANSCRIPTION FACTOR,EPITHELIAL-CELLS,PROTEIN PLAKOPHILIN-2,ACTIN CYTOSKELETON,FRAGILITY-SYNDROME},
  language     = {eng},
  number       = {4},
  pages        = {14},
  title        = {Plakophilin-3 is required for late embryonic amphibian development, exhibiting roles in ectodermal and neural tissues},
  url          = {http://dx.doi.org/10.1371/journal.pone.0034342},
  volume       = {7},
  year         = {2012},
}

Chicago
Munoz, William A, Malgorzata Kloc, Kyucheol Cho, Moonsup Lee, Ilse Hofmann, Amy Sater, Kris Vleminckx, and Pierre D McCrea. 2012. “Plakophilin-3 Is Required for Late Embryonic Amphibian Development, Exhibiting Roles in Ectodermal and Neural Tissues.” Plos One 7 (4).
APA
Munoz, W. A., Kloc, M., Cho, K., Lee, M., Hofmann, I., Sater, A., Vleminckx, K., et al. (2012). Plakophilin-3 is required for late embryonic amphibian development, exhibiting roles in ectodermal and neural tissues. PLOS ONE, 7(4).
Vancouver
1.
Munoz WA, Kloc M, Cho K, Lee M, Hofmann I, Sater A, et al. Plakophilin-3 is required for late embryonic amphibian development, exhibiting roles in ectodermal and neural tissues. PLOS ONE. 2012;7(4).
MLA
Munoz, William A, Malgorzata Kloc, Kyucheol Cho, et al. “Plakophilin-3 Is Required for Late Embryonic Amphibian Development, Exhibiting Roles in Ectodermal and Neural Tissues.” PLOS ONE 7.4 (2012): n. pag. Print.