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Plakophilin-3 is required for late embryonic amphibian development, exhibiting roles in ectodermal and neural tissues

(2012) PLOS ONE. 7(4).
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Abstract
The p120-catenin family has undergone a significant expansion during the evolution of vertebrates, resulting in varied functions that have yet to be discerned or fully characterized. Likewise, members of the plakophilins, a related catenin subfamily, are found throughout the cell with little known about their functions outside the desmosomal plaque. While the plakophilin-3 (Pkp3) knockout mouse resulted in skin defects, we find larger, including lethal effects following its depletion in Xenopus. Pkp3, unlike some other characterized catenins in amphibians, does not have significant maternal deposits of mRNA. However, during embryogenesis, two Pkp3 protein products whose temporal expression is partially complimentary become expressed. Only the smaller of these products is found in adult Xenopus tissues, with an expression pattern exhibiting distinctions as well as overlaps with those observed in mammalian studies. We determined that Xenopus Pkp3 depletion causes a skin fragility phenotype in keeping with the mouse knockout, but more novel, Xenopus tailbud embryos are hyposensitive to touch even in embryos lacking outward discernable phenotypes, and we additionally resolved disruptions in certain peripheral neural structures, altered establishment and migration of neural crest, and defects in ectodermal multiciliated cells. The use of two distinct morpholinos, as well as rescue approaches, indicated the specificity of these effects. Our results point to the requirement of Pkp3 in amphibian embryogenesis, with functional roles in a number of tissue types.
Keywords
DESMOSOMAL PLAQUE, CELL-ADHESION, RHO-FAMILY GTPASES, P120 CATENIN, BETA-CATENIN, TRANSCRIPTION FACTOR, EPITHELIAL-CELLS, PROTEIN PLAKOPHILIN-2, ACTIN CYTOSKELETON, FRAGILITY-SYNDROME

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Chicago
Munoz, William A, Malgorzata Kloc, Kyucheol Cho, Moonsup Lee, Ilse Hofmann, Amy Sater, Kris Vleminckx, and Pierre D McCrea. 2012. “Plakophilin-3 Is Required for Late Embryonic Amphibian Development, Exhibiting Roles in Ectodermal and Neural Tissues.” Plos One 7 (4).
APA
Munoz, W. A., Kloc, M., Cho, K., Lee, M., Hofmann, I., Sater, A., Vleminckx, K., et al. (2012). Plakophilin-3 is required for late embryonic amphibian development, exhibiting roles in ectodermal and neural tissues. PLOS ONE, 7(4).
Vancouver
1.
Munoz WA, Kloc M, Cho K, Lee M, Hofmann I, Sater A, et al. Plakophilin-3 is required for late embryonic amphibian development, exhibiting roles in ectodermal and neural tissues. PLOS ONE. 2012;7(4).
MLA
Munoz, William A, Malgorzata Kloc, Kyucheol Cho, et al. “Plakophilin-3 Is Required for Late Embryonic Amphibian Development, Exhibiting Roles in Ectodermal and Neural Tissues.” PLOS ONE 7.4 (2012): n. pag. Print.
@article{2941018,
  abstract     = {The p120-catenin family has undergone a significant expansion during the evolution of vertebrates, resulting in varied functions that have yet to be discerned or fully characterized. Likewise, members of the plakophilins, a related catenin subfamily, are found throughout the cell with little known about their functions outside the desmosomal plaque. While the plakophilin-3 (Pkp3) knockout mouse resulted in skin defects, we find larger, including lethal effects following its depletion in Xenopus. Pkp3, unlike some other characterized catenins in amphibians, does not have significant maternal deposits of mRNA. However, during embryogenesis, two Pkp3 protein products whose temporal expression is partially complimentary become expressed. Only the smaller of these products is found in adult Xenopus tissues, with an expression pattern exhibiting distinctions as well as overlaps with those observed in mammalian studies. We determined that Xenopus Pkp3 depletion causes a skin fragility phenotype in keeping with the mouse knockout, but more novel, Xenopus tailbud embryos are hyposensitive to touch even in embryos lacking outward discernable phenotypes, and we additionally resolved disruptions in certain peripheral neural structures, altered establishment and migration of neural crest, and defects in ectodermal multiciliated cells. The use of two distinct morpholinos, as well as rescue approaches, indicated the specificity of these effects. Our results point to the requirement of Pkp3 in amphibian embryogenesis, with functional roles in a number of tissue types.},
  articleno    = {e34342},
  author       = {Munoz, William A and Kloc, Malgorzata and Cho, Kyucheol and Lee, Moonsup and Hofmann, Ilse and Sater, Amy and Vleminckx, Kris and McCrea, Pierre D},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {DESMOSOMAL PLAQUE,CELL-ADHESION,RHO-FAMILY GTPASES,P120 CATENIN,BETA-CATENIN,TRANSCRIPTION FACTOR,EPITHELIAL-CELLS,PROTEIN PLAKOPHILIN-2,ACTIN CYTOSKELETON,FRAGILITY-SYNDROME},
  language     = {eng},
  number       = {4},
  pages        = {14},
  title        = {Plakophilin-3 is required for late embryonic amphibian development, exhibiting roles in ectodermal and neural tissues},
  url          = {http://dx.doi.org/10.1371/journal.pone.0034342},
  volume       = {7},
  year         = {2012},
}

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