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Ultrahigh-performance liquid chromatographic-tandem mass spectrometric multimycotoxin method for quantitating 26 mycotoxins in maize silage

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Abstract
A multianalyte method was developed to identify and quantitate 26 mycotoxins simultaneously in maize silage by means of ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The extraction and cleanup procedure consists of two extraction steps followed by purification on a Waters Oasis HLB column. The method developed was validated with the requirements of Commission Decision 2002/657/EC taken into account. The limit of detection and quantitation ranges were 5-348 and 11-695 ng/g, respectively. Apparent recovery varied between 61 and 116%, whereas repeatability and reproducibility were within the ranges of 3-45 and 5-49%, respectively. The method developed was successfully applied for maize silage samples taken at the cutting surface and 1 m behind that surface. Mainly Fusarium toxins (beauvericin, deoxynivalenol, enniatins, fumonisins, fusaric acid, and zearalenone) were detected, but postharvest toxins such as mycophenolic acid and roquefortine C were identified as well.
Keywords
UHPLC-MS/MS, validation, multimycotoxin analysis, FOOD, FEEDS, FUNGI, IMPACT, CATTLE, ANIMALS, VALIDATION, CORN-SILAGE, WHEAT, LC-MS/MS, maize silage

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Chicago
Van Pamel, Els, Annemieke Verbeken, Geertrui Vlaemynck, Johan De Boever, and Els Daeseleire. 2011. “Ultrahigh-performance Liquid Chromatographic-tandem Mass Spectrometric Multimycotoxin Method for Quantitating 26 Mycotoxins in Maize Silage.” Journal of Agricultural and Food Chemistry 59 (18): 9747–9755.
APA
Van Pamel, E., Verbeken, A., Vlaemynck, G., De Boever, J., & Daeseleire, E. (2011). Ultrahigh-performance liquid chromatographic-tandem mass spectrometric multimycotoxin method for quantitating 26 mycotoxins in maize silage. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 59(18), 9747–9755.
Vancouver
1.
Van Pamel E, Verbeken A, Vlaemynck G, De Boever J, Daeseleire E. Ultrahigh-performance liquid chromatographic-tandem mass spectrometric multimycotoxin method for quantitating 26 mycotoxins in maize silage. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2011;59(18):9747–55.
MLA
Van Pamel, Els, Annemieke Verbeken, Geertrui Vlaemynck, et al. “Ultrahigh-performance Liquid Chromatographic-tandem Mass Spectrometric Multimycotoxin Method for Quantitating 26 Mycotoxins in Maize Silage.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 59.18 (2011): 9747–9755. Print.
@article{2939784,
  abstract     = {A multianalyte method was developed to identify and quantitate 26 mycotoxins simultaneously in maize silage by means of ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The extraction and cleanup procedure consists of two extraction steps followed by purification on a Waters Oasis HLB column. The method developed was validated with the requirements of Commission Decision 2002/657/EC taken into account. The limit of detection and quantitation ranges were 5-348 and 11-695 ng/g, respectively. Apparent recovery varied between 61 and 116\%, whereas repeatability and reproducibility were within the ranges of 3-45 and 5-49\%, respectively. The method developed was successfully applied for maize silage samples taken at the cutting surface and 1 m behind that surface. Mainly Fusarium toxins (beauvericin, deoxynivalenol, enniatins, fumonisins, fusaric acid, and zearalenone) were detected, but postharvest toxins such as mycophenolic acid and roquefortine C were identified as well.},
  author       = {Van Pamel, Els and Verbeken, Annemieke and Vlaemynck, Geertrui and De Boever, Johan and Daeseleire, Els},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keyword      = {UHPLC-MS/MS,validation,multimycotoxin analysis,FOOD,FEEDS,FUNGI,IMPACT,CATTLE,ANIMALS,VALIDATION,CORN-SILAGE,WHEAT,LC-MS/MS,maize silage},
  language     = {eng},
  number       = {18},
  pages        = {9747--9755},
  title        = {Ultrahigh-performance liquid chromatographic-tandem mass spectrometric multimycotoxin method for quantitating 26 mycotoxins in maize silage},
  url          = {http://dx.doi.org/10.1021/jf202614h},
  volume       = {59},
  year         = {2011},
}

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