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Elucidating the mechanisms behind sonoporation with adeno-associated virus-loaded microbubbles

Bart Geers UGent, Ine Lentacker UGent, Angelika Alonso, Niek Sanders UGent, Jo Demeester UGent, Stephen Meairs and Stefaan De Smedt UGent (2011) MOLECULAR PHARMACEUTICS. 8(6). p.2244-2251
abstract
Microbubbles are Food and Drug Administration (FDA) approved contrast agents for ultrasound imaging. It has been reported that applying ultrasound on drug-loaded microbubbles facilitates drug uptake by cells, due to so-named sonoporation. However, the biophysics behind sonoporation are not fully understood. It is believed that sonoporation results in a "direct" delivery of drugs in the cytoplasm of cells, though it has been suggested as well that sonoporation facilitates endocytosis which would improve the internalization of drugs by cells. To get a better understanding of sonoporation, this study reports on the ultrasound assisted delivery of adeno-associated virus (AAV) loaded on the surface of microbubbles. AAVs rely on endocytosis for efficient transduction of cells and are, consequently, an elegant tool to evaluate whether endocytosis is involved in ultrasound-induced sonoporation. Applying ultrasound on AAV-loaded microbubbles dearly improved the internalization of AAVs by cells, though transduction of the cells did not occur, indicating that by sonoporation substances become directly delivered in the cytosol of cells.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
sonoporation, microbubbles, AAV, PEGylation, GENE-TRANSFER, ULTRASOUND EXPOSURE, CAVITATION BUBBLES, VIRAL VECTORS, DELIVERY, CELLS, DRUG, ENTRY, ultrasound
journal title
MOLECULAR PHARMACEUTICS
Mol. Pharm.
volume
8
issue
6
pages
2244 - 2251
Web of Science type
Article
Web of Science id
000297537300026
JCR category
PHARMACOLOGY & PHARMACY
JCR impact factor
4.782 (2011)
JCR rank
27/259 (2011)
JCR quartile
1 (2011)
ISSN
1543-8384
DOI
10.1021/mp200112y
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2938732
handle
http://hdl.handle.net/1854/LU-2938732
date created
2012-06-26 16:28:05
date last changed
2012-07-05 13:49:47
@article{2938732,
  abstract     = {Microbubbles are Food and Drug Administration (FDA) approved contrast agents for ultrasound imaging. It has been reported that applying ultrasound on drug-loaded microbubbles facilitates drug uptake by cells, due to so-named sonoporation. However, the biophysics behind sonoporation are not fully understood. It is believed that sonoporation results in a {\textacutedbl}direct{\textacutedbl} delivery of drugs in the cytoplasm of cells, though it has been suggested as well that sonoporation facilitates endocytosis which would improve the internalization of drugs by cells. To get a better understanding of sonoporation, this study reports on the ultrasound assisted delivery of adeno-associated virus (AAV) loaded on the surface of microbubbles. AAVs rely on endocytosis for efficient transduction of cells and are, consequently, an elegant tool to evaluate whether endocytosis is involved in ultrasound-induced sonoporation. Applying ultrasound on AAV-loaded microbubbles dearly improved the internalization of AAVs by cells, though transduction of the cells did not occur, indicating that by sonoporation substances become directly delivered in the cytosol of cells.},
  author       = {Geers, Bart and Lentacker, Ine and Alonso, Angelika and Sanders, Niek and Demeester, Jo and Meairs, Stephen and De Smedt, Stefaan},
  issn         = {1543-8384},
  journal      = {MOLECULAR PHARMACEUTICS},
  keyword      = {sonoporation,microbubbles,AAV,PEGylation,GENE-TRANSFER,ULTRASOUND EXPOSURE,CAVITATION BUBBLES,VIRAL VECTORS,DELIVERY,CELLS,DRUG,ENTRY,ultrasound},
  language     = {eng},
  number       = {6},
  pages        = {2244--2251},
  title        = {Elucidating the mechanisms behind sonoporation with adeno-associated virus-loaded microbubbles},
  url          = {http://dx.doi.org/10.1021/mp200112y},
  volume       = {8},
  year         = {2011},
}

Chicago
Geers, Bart, Ine Lentacker, Angelika Alonso, Niek Sanders, Jo Demeester, Stephen Meairs, and Stefaan De Smedt. 2011. “Elucidating the Mechanisms Behind Sonoporation with Adeno-associated Virus-loaded Microbubbles.” Molecular Pharmaceutics 8 (6): 2244–2251.
APA
Geers, B., Lentacker, I., Alonso, A., Sanders, N., Demeester, J., Meairs, S., & De Smedt, S. (2011). Elucidating the mechanisms behind sonoporation with adeno-associated virus-loaded microbubbles. MOLECULAR PHARMACEUTICS, 8(6), 2244–2251.
Vancouver
1.
Geers B, Lentacker I, Alonso A, Sanders N, Demeester J, Meairs S, et al. Elucidating the mechanisms behind sonoporation with adeno-associated virus-loaded microbubbles. MOLECULAR PHARMACEUTICS. 2011;8(6):2244–51.
MLA
Geers, Bart, Ine Lentacker, Angelika Alonso, et al. “Elucidating the Mechanisms Behind Sonoporation with Adeno-associated Virus-loaded Microbubbles.” MOLECULAR PHARMACEUTICS 8.6 (2011): 2244–2251. Print.