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Citrobacter werkmanii, a new candidate for the production of 1,3-propanediol: strain selection and carbon source optimization

Veerle Maervoet UGent, Joeri Beauprez UGent, Sofie De Maeseneire UGent, Wim Soetaert UGent and Marjan De Mey UGent (2012) GREEN CHEMISTRY. 14(8). p.2168-2178
abstract
The past decade 1,3-propanedial (PDO) has been elected as one of the top added value bio-based chemical building blocks in many reports, leading to Klebsiella sp., Clostridium sp., and Escherichia coli based production platforms. However, Citrobacter sp. are also known to naturally produce PDO. In this work a range of Citrobacter sp. has been screened for their PDO production capacity and their natural resistance against high PDO titers, leading to the selection of a new candidate for the production of PDO from glycerol, namely Citrobacter werkmanii DSM17579. In batch fermentation, a production rate of 2.84 g/L/h and a yield of 0.62 mol/mol glycerol was obtained starting from 60 g/L and 20 g/L glycerol, respectively. The metabolism of this organism was further studied by perturbing it with 18 carbon sources as co-substrates. These results pointed to the potential use of cheap waste streams such as ligno/hemicellulosic hydrolysates for the production of PDO. Furthermore, the sugar alcohol D-mannitol and D-galactose enhance the production yield significantly (0.83 mol/mol and 0.81 mol/mol, respectively, an enhancement of about 30 % compared to glycerol as sole carbon source). The latter indicates the potential of whey based waste streams for the production of PDO. These are, to date, the highest yields reported for natural producing Enterobacteriaceae using co-substrates for the production of PDO from glycerol.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
1_3-Propanediol, Citrobacter werkmanii, Co-substrate, Inhibition, Glycerol, ESCHERICHIA-COLI, CLOSTRIDIUM-BUTYRICUM, KLEBSIELLA-PNEUMONIAE, CRUDE GLYCEROL, MICROBIAL-PRODUCTION, FERMENTATION, FREUNDII, PURIFICATION, INHIBITION, GLUCOSE
journal title
GREEN CHEMISTRY
Green Chem.
volume
14
issue
8
pages
2168 - 2178
Web of Science type
Article
Web of Science id
000306658200012
JCR category
CHEMISTRY, MULTIDISCIPLINARY
JCR impact factor
6.828 (2012)
JCR rank
18/150 (2012)
JCR quartile
1 (2012)
ISSN
1463-9262
DOI
10.1039/c2gc35369e
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2917695
handle
http://hdl.handle.net/1854/LU-2917695
date created
2012-06-25 11:18:53
date last changed
2014-05-26 10:03:29
@article{2917695,
  abstract     = {The past decade 1,3-propanedial (PDO) has been elected as one of the top added value bio-based chemical building blocks in many reports, leading to Klebsiella sp., Clostridium sp., and Escherichia coli based production platforms. However, Citrobacter sp. are also known to naturally produce PDO. In this work a range of Citrobacter sp. has been screened for their PDO production capacity and their natural resistance against high PDO titers, leading to the selection of a new candidate for the production of PDO from glycerol, namely Citrobacter werkmanii DSM17579. In batch fermentation, a production rate of 2.84 g/L/h and a yield of 0.62 mol/mol glycerol was obtained starting from 60 g/L and 20 g/L glycerol, respectively. The metabolism of this organism was further studied by perturbing it with 18 carbon sources as co-substrates. These results pointed to the potential use of cheap waste streams such as ligno/hemicellulosic hydrolysates for the production of PDO. Furthermore, the sugar alcohol D-mannitol and D-galactose enhance the production yield significantly (0.83 mol/mol and 0.81 mol/mol, respectively, an enhancement of about 30 \% compared to glycerol as sole carbon source). The latter indicates the potential of whey based waste streams for the production of PDO. These are, to date, the highest yields reported for natural producing Enterobacteriaceae using co-substrates for the production of PDO from glycerol.},
  author       = {Maervoet, Veerle and Beauprez, Joeri and De Maeseneire, Sofie and Soetaert, Wim and De Mey, Marjan},
  issn         = {1463-9262},
  journal      = {GREEN CHEMISTRY},
  keyword      = {1\_3-Propanediol,Citrobacter werkmanii,Co-substrate,Inhibition,Glycerol,ESCHERICHIA-COLI,CLOSTRIDIUM-BUTYRICUM,KLEBSIELLA-PNEUMONIAE,CRUDE GLYCEROL,MICROBIAL-PRODUCTION,FERMENTATION,FREUNDII,PURIFICATION,INHIBITION,GLUCOSE},
  language     = {eng},
  number       = {8},
  pages        = {2168--2178},
  title        = {Citrobacter werkmanii, a new candidate for the production of 1,3-propanediol: strain selection and carbon source optimization},
  url          = {http://dx.doi.org/10.1039/c2gc35369e},
  volume       = {14},
  year         = {2012},
}

Chicago
Maervoet, Veerle, Joeri Beauprez, Sofie De Maeseneire, Wim Soetaert, and Marjan De Mey. 2012. “Citrobacter Werkmanii, a New Candidate for the Production of 1,3-propanediol: Strain Selection and Carbon Source Optimization.” Green Chemistry 14 (8): 2168–2178.
APA
Maervoet, V., Beauprez, J., De Maeseneire, S., Soetaert, W., & De Mey, M. (2012). Citrobacter werkmanii, a new candidate for the production of 1,3-propanediol: strain selection and carbon source optimization. GREEN CHEMISTRY, 14(8), 2168–2178.
Vancouver
1.
Maervoet V, Beauprez J, De Maeseneire S, Soetaert W, De Mey M. Citrobacter werkmanii, a new candidate for the production of 1,3-propanediol: strain selection and carbon source optimization. GREEN CHEMISTRY. 2012;14(8):2168–78.
MLA
Maervoet, Veerle, Joeri Beauprez, Sofie De Maeseneire, et al. “Citrobacter Werkmanii, a New Candidate for the Production of 1,3-propanediol: Strain Selection and Carbon Source Optimization.” GREEN CHEMISTRY 14.8 (2012): 2168–2178. Print.