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The fatty acid profile of subcutaneous and abdominal fat in dairy cows with left displacement of the abomasum

Miel Hostens UGent, Veerle Fievez UGent, JLMR Leroy, Gijs Van Ranst UGent, Bruno Vlaeminck UGent and Geert Opsomer UGent (2012) JOURNAL OF DAIRY SCIENCE. 95(7). p.3756-3765
abstract
The objective of this study was to determine the fatty acid (FA) profile and assess desaturase indices of nonesterified fatty acids (NEFA) in the blood, as well as in the abdominal (ABD) and subcutaneous (SUBC) fat stores, in dairy cows with left displacement of the abomasum (LDA). Blood, ABD, and SUBC samples were taken from 50 Holstein cows offered for surgery to correct LDA. The FA profile of the 3 compartments was determined by gas chromatography after lipid extraction, methylation, and, in the case of blood plasma, separation of lipid classes. The most abundant FA in all 3 compartments were 16:0, 18:0, and 18:1 cis-9, with a total proportion of 82.5, 68.0, and 74.1 g/100 g of FA in ABD, NEFA, and SUBC, respectively. A principal component analysis was performed on the entire FA profile as well as on the Δ9-desaturase indices (14:1 cis-9/14:0, 16:1 cis-9/16:0, 18:1 cis-9/18:0). The principal component analysis extracted 2 principal components (PC), representing 51.6% (PC1) and 21.1% (PC2) of the total variance in FA composition of the 3 compartments. The loading plot for the regression factors revealed a strong positive correlation between PC1 with the Δ9-desaturase indices and the proportions of 14:1 cis-9 and 16:1 cis-9, and revealed a negative correlation with the proportion of 18:0 and saturated FA. The correlation with PC2 was positive for the proportion of unsaturated FA, 18:2n-6, and 18:3n-3, and negative for the proportion of 14:0, 16:0, and saturated FA. The SUBC could be distinguished from the NEFA and ABD by a positive score for PC1, whereas differentiation among the latter 2 compartments could be made by a positive (NEFA) or negative (ABD) score for PC2. The Δ9-desaturase indices for C14 and C16 differed between all compartments but were numerically closer for NEFA and ABD versus NEFA and SUBC. The desaturase indices of the main FA (18:1 cis-9 and 18:0) did not differ between NEFA and ABD. These results support the existence of a different FA composition in ABD compared with SUBC. The greater similarity between the FA profiles of ABD and NEFA compared with SUBC and NEFA and the closer desaturase indices of ABD and NEFA support the hypothesis of a preferential mobilization of ABD fat in dairy cows with LDA.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
subcutaneous fat, cow, nonesterified fatty acids, abdominal fat, CONJUGATED LINOLEIC-ACID, ADIPOSE-TISSUE, FOLLICULAR-FLUID, ENZYME-ACTIVITIES, ADIPOCYTE SIZE, LIVER LIPIDS, BLOOD-FLOW, IN-VITRO, PLASMA, STEERS
journal title
JOURNAL OF DAIRY SCIENCE
J. Dairy Sci.
volume
95
issue
7
pages
3756 - 3765
Web of Science type
Article
Web of Science id
000305363100026
JCR category
AGRICULTURE, DAIRY & ANIMAL SCIENCE
JCR impact factor
2.566 (2012)
JCR rank
3/54 (2012)
JCR quartile
1 (2012)
ISSN
0022-0302
DOI
10.3168/jds.2011-5092
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2917572
handle
http://hdl.handle.net/1854/LU-2917572
date created
2012-06-25 10:23:31
date last changed
2012-09-28 15:35:30
@article{2917572,
  abstract     = {The objective of this study was to determine the fatty acid (FA) profile and assess desaturase indices of nonesterified fatty acids (NEFA) in the blood, as well as in the abdominal (ABD) and subcutaneous (SUBC) fat stores, in dairy cows with left displacement of the abomasum (LDA). Blood, ABD, and SUBC samples were taken from 50 Holstein cows offered for surgery to correct LDA. The FA profile of the 3 compartments was determined by gas chromatography after lipid extraction, methylation, and, in the case of blood plasma, separation of lipid classes. The most abundant FA in all 3 compartments were 16:0, 18:0, and 18:1 cis-9, with a total proportion of 82.5, 68.0, and 74.1 g/100 g of FA in ABD, NEFA, and SUBC, respectively. A principal component analysis was performed on the entire FA profile as well as on the \ensuremath{\Delta}9-desaturase indices (14:1 cis-9/14:0, 16:1 cis-9/16:0, 18:1 cis-9/18:0). The principal component analysis extracted 2 principal components (PC), representing 51.6\% (PC1) and 21.1\% (PC2) of the total variance in FA composition of the 3 compartments. The loading plot for the regression factors revealed a strong positive correlation between PC1 with the \ensuremath{\Delta}9-desaturase indices and the proportions of 14:1 cis-9 and 16:1 cis-9, and revealed a negative correlation with the proportion of 18:0 and saturated FA. The correlation with PC2 was positive for the proportion of unsaturated FA, 18:2n-6, and 18:3n-3, and negative for the proportion of 14:0, 16:0, and saturated FA. The SUBC could be distinguished from the NEFA and ABD by a positive score for PC1, whereas differentiation among the latter 2 compartments could be made by a positive (NEFA) or negative (ABD) score for PC2. The \ensuremath{\Delta}9-desaturase indices for C14 and C16 differed between all compartments but were numerically closer for NEFA and ABD versus NEFA and SUBC. The desaturase indices of the main FA (18:1 cis-9 and 18:0) did not differ between NEFA and ABD. These results support the existence of a different FA composition in ABD compared with SUBC. The greater similarity between the FA profiles of ABD and NEFA compared with SUBC and NEFA and the closer desaturase indices of ABD and NEFA support the hypothesis of a preferential mobilization of ABD fat in dairy cows with LDA.},
  author       = {Hostens, Miel and Fievez, Veerle and Leroy, JLMR and Van Ranst, Gijs and Vlaeminck, Bruno and Opsomer, Geert},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keyword      = {subcutaneous fat,cow,nonesterified fatty acids,abdominal fat,CONJUGATED LINOLEIC-ACID,ADIPOSE-TISSUE,FOLLICULAR-FLUID,ENZYME-ACTIVITIES,ADIPOCYTE SIZE,LIVER LIPIDS,BLOOD-FLOW,IN-VITRO,PLASMA,STEERS},
  language     = {eng},
  number       = {7},
  pages        = {3756--3765},
  title        = {The fatty acid profile of subcutaneous and abdominal fat in dairy cows with left displacement of the abomasum},
  url          = {http://dx.doi.org/10.3168/jds.2011-5092},
  volume       = {95},
  year         = {2012},
}

Chicago
Hostens, Miel, Veerle Fievez, JLMR Leroy, Gijs Van Ranst, Bruno Vlaeminck, and Geert Opsomer. 2012. “The Fatty Acid Profile of Subcutaneous and Abdominal Fat in Dairy Cows with Left Displacement of the Abomasum.” Journal of Dairy Science 95 (7): 3756–3765.
APA
Hostens, M., Fievez, V., Leroy, J., Van Ranst, G., Vlaeminck, B., & Opsomer, G. (2012). The fatty acid profile of subcutaneous and abdominal fat in dairy cows with left displacement of the abomasum. JOURNAL OF DAIRY SCIENCE, 95(7), 3756–3765.
Vancouver
1.
Hostens M, Fievez V, Leroy J, Van Ranst G, Vlaeminck B, Opsomer G. The fatty acid profile of subcutaneous and abdominal fat in dairy cows with left displacement of the abomasum. JOURNAL OF DAIRY SCIENCE. 2012;95(7):3756–65.
MLA
Hostens, Miel, Veerle Fievez, JLMR Leroy, et al. “The Fatty Acid Profile of Subcutaneous and Abdominal Fat in Dairy Cows with Left Displacement of the Abomasum.” JOURNAL OF DAIRY SCIENCE 95.7 (2012): 3756–3765. Print.