@article{2916971,
  abstract     = {{Background: Human T-cell leukemia virus type 1 (HTLV-1) and type 2 both target T lymphocytes, yet induce radically different phenotypic outcomes. HTLV-1 is a causative agent of Adult T-cell leukemia (ATL), whereas HTLV-2, highly similar to HTLV-1, causes no known overt disease. HTLV gene products are engaged in a dynamic struggle of activating and antagonistic interactions with host cells. Investigations focused on one or a few genes have identified several human factors interacting with HTLV viral proteins. Most of the available interaction data concern the highly investigated HTLV-1 Tax protein. Identifying shared and distinct host-pathogen protein interaction profiles for these two viruses would enlighten how they exploit distinctive or common strategies to subvert cellular pathways toward disease progression.
Results: We employ a scalable methodology for the systematic mapping and comparison of pathogen-host protein interactions that includes stringent yeast two-hybrid screening and systematic retest, as well as two independent validations through an additional protein interaction detection method and a functional transactivation assay. The final data set contained 166 interactions between 10 viral proteins and 122 human proteins. Among the 166 interactions identified, 87 and 79 involved HTLV-1 and HTLV-2 -encoded proteins, respectively. Targets for HTLV-1 and HTLV-2 proteins implicate a diverse set of cellular processes including the ubiquitin-proteasome system, the apoptosis, different cancer pathways and the Notch signaling pathway.
Conclusions: This study constitutes a first pass, with homogeneous data, at comparative analysis of host targets for HTLV-1 and -2 retroviruses, complements currently existing data for formulation of systems biology models of retroviral induced diseases and presents new insights on biological pathways involved in retroviral infection.}},
  articleno    = {{26}},
  author       = {{Simonis, Nicolas and Rual, Jean-François and Lemmens, Irma and Boxus, Mathieu and Hirozane-Kishikawa, Tomoko and Gatot, Jean-Stéphane and Dricot, Amélie and Hao, Tong and Vertommen, Didier and Legros, Sébastien and Daakour, Sarah and Klitgord, Niels and Martin, Maud and Willaert, Jean-François and Dequiedt, Franck and Navratil, Vincent and Cusick, Michael E and Burny, Arsène and Van Lint, Carine and Hill, David E and Tavernier, Jan and Kettmann, Richard and Vidal, Marc and Twizere, Jean-Claude}},
  issn         = {{1742-4690}},
  journal      = {{RETROVIROLOGY}},
  keywords     = {{TRANSCRIPTION FACTORS,INTERACTION DATABASE,PROTEIN-INTERACTION NETWORK,ANAPHASE-PROMOTING COMPLEX,VIRUS TYPE-1 TAX,T-CELL LEUKEMIA,HBZ,Tax,ORFeome,Retrovirus,HTLV,Interactome,NUCLEOTIDE-SEQUENCE,GENE-EXPRESSION,FANCONI-ANEMIA,RNA-BINDING}},
  language     = {{eng}},
  pages        = {{20}},
  title        = {{Host-pathogen interactome mapping for HTLV-1 and -2 retroviruses}},
  url          = {{http://dx.doi.org/10.1186/1742-4690-9-26}},
  volume       = {{9}},
  year         = {{2012}},
}

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