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Intramyocardial implantation of bone marrow-derived stem cells enhances perfusion in chronic myocardial infarction: dependency on initial perfusion depth and follow-up assessed by gated pinhole SPECT

(2007) JOURNAL OF NUCLEAR MEDICINE. 48(3). p.405-412
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Abstract
Cell therapy-induced changes in the perfusion of areas of myocardial infarction (MI) remain unclear. This study investigated whether an original pinhole SPECT technique could be applied to a rat Ml model to analyze local improvement in myocardial perfusion relating to engraftment sites of bone marrow-derived stem cells (BMSCs). Methods: Four-month-old MI rats were either untreated (n = 8) or treated (n = 10) by intramyocardial injection of (111)in-labeled BMSCs. Early distribution of In-111-BMSCs within the MI target was evidenced by dual In-111/Tc-99m pinhole SPECT 48 h later. Myocardial perfusion was serially monitored by Tc-99m-sestamibi pinhole gated SPECT up to 3 mo after transplantation. Results: Forty-eight hours after transplantation, In-111-BMSCs were observed in all treated rats and in 18 of their 32 underperfused MI segments (< 70% sestamibi uptake before transplantation). During the subsequent 3-mo follow-up, the perfusion of MI segments worsened in untreated rats (absolute change in sestamibi uptake, -3% +/- 3%; P < 0.05) but improved in treated rats (+4% +/- 7%; P < 0.05). This perfusion improvement was unrelated to the initial detection of In-111-BMSCs (+2% +/- 6% in segments with In-111-BMSCs vs. +5% +/- 7% in those without; not statistically significant) but was strongly associated with less severe perfusion defects before transplantation (+6% +/- 6% in segments with 60%-70% sestamibi uptake [n = 19] vs. 1% +/- 6% in those with < 60% uptake [n = 13]; P = 0.003). Conclusion: When BMSCs are injected within chronic Ml, perfusion enhancement predominates in the MI areas showing a high enough residual perfusion before treatment but not in those of the initial cell engraftment, giving evidence of dependency on the perfusion and metabolic environment at implantation sites.
Keywords
COLLATERAL PERFUSION, ISCHEMIC-MYOCARDIUM, IMPROVES CARDIAC-FUNCTION, ENDOTHELIAL PROGENITOR CELLS, pinhole SPECT, In-111-oxine, sestamibi, bone marrow mesenchymal stem cells, stem cell therapy, myocardial infarction, rats, HEART, NEOVASCULARIZATION, TRANSPLANTATION, MODEL, SIZE, RECONSTRUCTION

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Citation

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Chicago
Tran, Nguyen, Philippe R Franken, Fatiha Maskali, Joseph Nloga, Pablo Maureira, Sylvain Poussier, Frederique Groubatch, Christian Vanhove, Jean-Pierre Villemot, and Pierre-Yves Marie. 2007. “Intramyocardial Implantation of Bone Marrow-derived Stem Cells Enhances Perfusion in Chronic Myocardial Infarction: Dependency on Initial Perfusion Depth and Follow-up Assessed by Gated Pinhole SPECT.” Journal of Nuclear Medicine 48 (3): 405–412.
APA
Tran, N., Franken, P. R., Maskali, F., Nloga, J., Maureira, P., Poussier, S., Groubatch, F., et al. (2007). Intramyocardial implantation of bone marrow-derived stem cells enhances perfusion in chronic myocardial infarction: dependency on initial perfusion depth and follow-up assessed by gated pinhole SPECT. JOURNAL OF NUCLEAR MEDICINE, 48(3), 405–412.
Vancouver
1.
Tran N, Franken PR, Maskali F, Nloga J, Maureira P, Poussier S, et al. Intramyocardial implantation of bone marrow-derived stem cells enhances perfusion in chronic myocardial infarction: dependency on initial perfusion depth and follow-up assessed by gated pinhole SPECT. JOURNAL OF NUCLEAR MEDICINE. 2007;48(3):405–12.
MLA
Tran, Nguyen, Philippe R Franken, Fatiha Maskali, et al. “Intramyocardial Implantation of Bone Marrow-derived Stem Cells Enhances Perfusion in Chronic Myocardial Infarction: Dependency on Initial Perfusion Depth and Follow-up Assessed by Gated Pinhole SPECT.” JOURNAL OF NUCLEAR MEDICINE 48.3 (2007): 405–412. Print.
@article{2916006,
  abstract     = {Cell therapy-induced changes in the perfusion of areas of myocardial infarction (MI) remain unclear. This study investigated whether an original pinhole SPECT technique could be applied to a rat Ml model to analyze local improvement in myocardial perfusion relating to engraftment sites of bone marrow-derived stem cells (BMSCs). Methods: Four-month-old MI rats were either untreated (n = 8) or treated (n = 10) by intramyocardial injection of (111)in-labeled BMSCs. Early distribution of In-111-BMSCs within the MI target was evidenced by dual In-111/Tc-99m pinhole SPECT 48 h later. Myocardial perfusion was serially monitored by Tc-99m-sestamibi pinhole gated SPECT up to 3 mo after transplantation. Results: Forty-eight hours after transplantation, In-111-BMSCs were observed in all treated rats and in 18 of their 32 underperfused MI segments (< 70% sestamibi uptake before transplantation). During the subsequent 3-mo follow-up, the perfusion of MI segments worsened in untreated rats (absolute change in sestamibi uptake, -3% +/- 3%; P < 0.05) but improved in treated rats (+4% +/- 7%; P < 0.05). This perfusion improvement was unrelated to the initial detection of In-111-BMSCs (+2% +/- 6% in segments with In-111-BMSCs vs. +5% +/- 7% in those without; not statistically significant) but was strongly associated with less severe perfusion defects before transplantation (+6% +/- 6% in segments with 60%-70% sestamibi uptake [n = 19] vs. 1% +/- 6% in those with < 60% uptake [n = 13]; P = 0.003). Conclusion: When BMSCs are injected within chronic Ml, perfusion enhancement predominates in the MI areas showing a high enough residual perfusion before treatment but not in those of the initial cell engraftment, giving evidence of dependency on the perfusion and metabolic environment at implantation sites.},
  author       = {Tran, Nguyen and Franken, Philippe R and Maskali, Fatiha and Nloga, Joseph and Maureira, Pablo and Poussier, Sylvain and Groubatch, Frederique and Vanhove, Christian and Villemot, Jean-Pierre and Marie, Pierre-Yves},
  issn         = {0161-5505},
  journal      = {JOURNAL OF NUCLEAR MEDICINE},
  keywords     = {COLLATERAL PERFUSION,ISCHEMIC-MYOCARDIUM,IMPROVES CARDIAC-FUNCTION,ENDOTHELIAL PROGENITOR CELLS,pinhole SPECT,In-111-oxine,sestamibi,bone marrow mesenchymal stem cells,stem cell therapy,myocardial infarction,rats,HEART,NEOVASCULARIZATION,TRANSPLANTATION,MODEL,SIZE,RECONSTRUCTION},
  language     = {eng},
  number       = {3},
  pages        = {405--412},
  title        = {Intramyocardial implantation of bone marrow-derived stem cells enhances perfusion in chronic myocardial infarction: dependency on initial perfusion depth and follow-up assessed by gated pinhole SPECT},
  url          = {http://jnm.snmjournals.org/content/48/3/405.abstract},
  volume       = {48},
  year         = {2007},
}

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