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Rab27B, an ex(o)citing driver of breast cancer growth, invasion and metastasis

An Hendrix UGent, Wendy Westbroek, Marc Bracke UGent and Olivier De Wever UGent (2012) JOURNAL OF EXTRACELLULAR VESICLES. 1(suppl.). p.77-77
abstract
Cancer cells implement various exocytic routes, modulated by small Rab GTPases, to relay crucial information for fostering growth, invasion and matrix degradation. We investigated the biological role and expression status of Rab27B, a regulator of exosome release, in breast cancer. Rab27B-upregulation in estrogen receptor (ER)-positive breast cancer cells promoted G1/S phase cell cycle transition and increased proliferation, F-actin reorganization and invasion in cell culture and invasive tumor growth and hemorrhagic ascites in a xenograft mouse model. Proteomics of purified Rab27B vesicles and the secretome of Rab27B-expressing breast cancer cells were identified HSP90a as key proinvasive growth regulator. HSP90a secretion occurred in a Rab27B-dependent manner and was required for MMP-2 activation. Endogenous Rab27B mRNA and protein, but not Rab3D and Rab27A mRNA, was associated with lymph node metastasis and differentiation grade in ER-positive breast cancer samples. In conclusion, Rab27B regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans. Because of the relationship between Rab27B and cancer progression, elucidating the role of exosomes in metastatic niche formation will be the next step forward in cancer research.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
JOURNAL OF EXTRACELLULAR VESICLES
J. Extracell. Vesicles
volume
1
issue
suppl.
pages
77 - 77
conference name
1st International meeting of the International Society for Extracellular Vesicles (ISEV 2012)
conference location
Göteborg, Sweden
conference start
2012-04-18
conference end
2012-04-21
ISSN
2001-3078
DOI
10.3402/jev.v1i0.18177
language
English
UGent publication?
yes
classification
C3
id
2915616
handle
http://hdl.handle.net/1854/LU-2915616
date created
2012-06-22 11:21:32
date last changed
2012-07-13 11:59:05
@inproceedings{2915616,
  abstract     = {Cancer cells implement various exocytic routes, modulated by small Rab GTPases, to relay crucial information for fostering growth, invasion and matrix degradation. We investigated the biological role and expression status of Rab27B, a regulator of exosome release, in breast cancer. Rab27B-upregulation in estrogen receptor (ER)-positive breast cancer cells promoted G1/S phase cell cycle transition and increased proliferation, F-actin reorganization and invasion in cell culture and invasive tumor growth and hemorrhagic ascites in a xenograft mouse model. Proteomics of purified Rab27B vesicles and the secretome of Rab27B-expressing breast cancer cells were identified HSP90a as key proinvasive growth regulator. HSP90a secretion occurred in a Rab27B-dependent manner and was required for MMP-2 activation. Endogenous Rab27B mRNA and protein, but not Rab3D and Rab27A mRNA, was associated with lymph node metastasis and differentiation grade in ER-positive breast cancer samples. In conclusion, Rab27B regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans. Because of the relationship between Rab27B and cancer progression, elucidating the role of exosomes in metastatic niche formation will be the next step forward in cancer research.},
  author       = {Hendrix, An and Westbroek, Wendy and Bracke, Marc and De Wever, Olivier},
  booktitle    = {JOURNAL OF EXTRACELLULAR VESICLES},
  issn         = {2001-3078},
  language     = {eng},
  location     = {G{\"o}teborg, Sweden},
  number       = {suppl.},
  pages        = {77--77},
  title        = {Rab27B, an ex(o)citing driver of breast cancer growth, invasion and metastasis},
  url          = {http://dx.doi.org/10.3402/jev.v1i0.18177},
  volume       = {1},
  year         = {2012},
}

Chicago
Hendrix, An, Wendy Westbroek, Marc Bracke, and Olivier De Wever. 2012. “Rab27B, an Ex(o)citing Driver of Breast Cancer Growth, Invasion and Metastasis.” In Journal of Extracellular Vesicles, 1:77–77.
APA
Hendrix, A., Westbroek, W., Bracke, M., & De Wever, O. (2012). Rab27B, an ex(o)citing driver of breast cancer growth, invasion and metastasis. JOURNAL OF EXTRACELLULAR VESICLES (Vol. 1, pp. 77–77). Presented at the 1st International meeting of the International Society for Extracellular Vesicles (ISEV 2012).
Vancouver
1.
Hendrix A, Westbroek W, Bracke M, De Wever O. Rab27B, an ex(o)citing driver of breast cancer growth, invasion and metastasis. JOURNAL OF EXTRACELLULAR VESICLES. 2012. p. 77–77.
MLA
Hendrix, An, Wendy Westbroek, Marc Bracke, et al. “Rab27B, an Ex(o)citing Driver of Breast Cancer Growth, Invasion and Metastasis.” Journal of Extracellular Vesicles. Vol. 1. 2012. 77–77. Print.