Advanced search
1 file | 177.67 KB Add to list

Virulence profiling and quantification of verocytotoxin-producing Escherichia coli O145:H28 and O26:H11 isolated during an ice cream-related hemolytic uremic syndrome outbreak

Author
Organization
Abstract
In September-October 2007, a mixed-serotype outbreak of verocytotoxin-producing Escherichia coli (VTEC) O145:H28 and O26:H11 occurred in the province of Antwerp, Belgium. Five girls aged between 2 and 11 years developed hemolytic uremic syndrome, and seven other coexposed persons with bloody diarrhea were identified. Laboratory confirmation of O145:H28 infection was obtained for three hemolytic uremic syndrome patients, one of whom was coinfected with O26:H11. The epidemiological and laboratory investigations revealed ice cream as the most likely source of the outbreak. The ice cream was produced at a local dairy farm using pasteurized milk. VTEC of both serotypes with indistinguishable pulsed-field gel electrophoresis patterns were isolated from patients, ice cream, and environmental samples. Quantitative analysis of the ice cream indicated concentrations of 2.4 and 0.03 CFU/g for VTEC O145 and O26, respectively. Virulence typing revealed that the repertoire of virulence genes carried by the O145:H28 outbreak strain was comparable to that of O157 VTEC and more exhaustive as compared to the O26:H11 outbreak strain and nonrelated clinical strains belonging to these serotypes. Taken together, these data suggest that O145:H28 played the most important role in this outbreak.
Keywords
COINFECTION, JAPAN, O26-H11, INFECTION, SEROTYPES, MULTIPLEX PCR, O157, STX(2), STEC, O111

Downloads

  • G Buvens.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 177.67 KB

Citation

Please use this url to cite or link to this publication:

MLA
Buvens, Glenn, et al. “Virulence Profiling and Quantification of Verocytotoxin-Producing Escherichia Coli O145:H28 and O26:H11 Isolated during an Ice Cream-Related Hemolytic Uremic Syndrome Outbreak.” FOODBORNE PATHOGENS AND DISEASE, vol. 8, no. 3, 2011, pp. 421–26, doi:10.1089/fpd.2010.0693.
APA
Buvens, G., Possé, B., De Schrijver, K., De Zutter, L., Lauwers, S., & Pierard, D. (2011). Virulence profiling and quantification of verocytotoxin-producing Escherichia coli O145:H28 and O26:H11 isolated during an ice cream-related hemolytic uremic syndrome outbreak. FOODBORNE PATHOGENS AND DISEASE, 8(3), 421–426. https://doi.org/10.1089/fpd.2010.0693
Chicago author-date
Buvens, Glenn, Björn Possé, Koen De Schrijver, Lieven De Zutter, Sabine Lauwers, and Denis Pierard. 2011. “Virulence Profiling and Quantification of Verocytotoxin-Producing Escherichia Coli O145:H28 and O26:H11 Isolated during an Ice Cream-Related Hemolytic Uremic Syndrome Outbreak.” FOODBORNE PATHOGENS AND DISEASE 8 (3): 421–26. https://doi.org/10.1089/fpd.2010.0693.
Chicago author-date (all authors)
Buvens, Glenn, Björn Possé, Koen De Schrijver, Lieven De Zutter, Sabine Lauwers, and Denis Pierard. 2011. “Virulence Profiling and Quantification of Verocytotoxin-Producing Escherichia Coli O145:H28 and O26:H11 Isolated during an Ice Cream-Related Hemolytic Uremic Syndrome Outbreak.” FOODBORNE PATHOGENS AND DISEASE 8 (3): 421–426. doi:10.1089/fpd.2010.0693.
Vancouver
1.
Buvens G, Possé B, De Schrijver K, De Zutter L, Lauwers S, Pierard D. Virulence profiling and quantification of verocytotoxin-producing Escherichia coli O145:H28 and O26:H11 isolated during an ice cream-related hemolytic uremic syndrome outbreak. FOODBORNE PATHOGENS AND DISEASE. 2011;8(3):421–6.
IEEE
[1]
G. Buvens, B. Possé, K. De Schrijver, L. De Zutter, S. Lauwers, and D. Pierard, “Virulence profiling and quantification of verocytotoxin-producing Escherichia coli O145:H28 and O26:H11 isolated during an ice cream-related hemolytic uremic syndrome outbreak,” FOODBORNE PATHOGENS AND DISEASE, vol. 8, no. 3, pp. 421–426, 2011.
@article{2915108,
  abstract     = {{In September-October 2007, a mixed-serotype outbreak of verocytotoxin-producing Escherichia coli (VTEC) O145:H28 and O26:H11 occurred in the province of Antwerp, Belgium. Five girls aged between 2 and 11 years developed hemolytic uremic syndrome, and seven other coexposed persons with bloody diarrhea were identified. Laboratory confirmation of O145:H28 infection was obtained for three hemolytic uremic syndrome patients, one of whom was coinfected with O26:H11. The epidemiological and laboratory investigations revealed ice cream as the most likely source of the outbreak. The ice cream was produced at a local dairy farm using pasteurized milk. VTEC of both serotypes with indistinguishable pulsed-field gel electrophoresis patterns were isolated from patients, ice cream, and environmental samples. Quantitative analysis of the ice cream indicated concentrations of 2.4 and 0.03 CFU/g for VTEC O145 and O26, respectively. Virulence typing revealed that the repertoire of virulence genes carried by the O145:H28 outbreak strain was comparable to that of O157 VTEC and more exhaustive as compared to the O26:H11 outbreak strain and nonrelated clinical strains belonging to these serotypes. Taken together, these data suggest that O145:H28 played the most important role in this outbreak.}},
  author       = {{Buvens, Glenn and Possé, Björn and De Schrijver, Koen and De Zutter, Lieven and Lauwers, Sabine and Pierard, Denis}},
  issn         = {{1535-3141}},
  journal      = {{FOODBORNE PATHOGENS AND DISEASE}},
  keywords     = {{COINFECTION,JAPAN,O26-H11,INFECTION,SEROTYPES,MULTIPLEX PCR,O157,STX(2),STEC,O111}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{421--426}},
  title        = {{Virulence profiling and quantification of verocytotoxin-producing Escherichia coli O145:H28 and O26:H11 isolated during an ice cream-related hemolytic uremic syndrome outbreak}},
  url          = {{http://doi.org/10.1089/fpd.2010.0693}},
  volume       = {{8}},
  year         = {{2011}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: