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SPEECHLESS integrates brassinosteroid and stomata signalling pathways

(2012) NATURE CELL BIOLOGY. 14(5). p.548-U214
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Abstract
Stomatal formation is regulated by multiple developmental and environmental signals, but how these signals are integrated to control this process is not fully understood(1). In Arabidopsis thaliana, the basic helix-loop-helix transcription factor SPEECHLESS (SPCH) regulates the entry, amplifying and spacing divisions that occur during stomatal lineage development. SPCH activity is negatively regulated by mitogen-activated protein kinase (MAPK)-mediated phosphorylation(2). Here, we show that in addition to MAPKs, SPCH activity is also modulated by brassinosteroid (BR) signalling. The GSK3/SHAGGY-like kinase BIN2 (BR INSENSITIVE2) phosphorylates residues overlapping those targeted by the MAPKs, as well as four residues in the amino-terminal region of the protein outside the MAPK target domain. These phosphorylation events antagonize SPCH activity and limit epidermal cell proliferation. Conversely, inhibition of BIN2 activity in vivo stabilizes SPCH and triggers excessive stomatal and non-stomatal cell formation. We demonstrate that through phosphorylation inputs from both MAPKs and BIN2, SPCH serves as an integration node for stomata and BR signalling pathways to control stomatal development in Arabidopsis.
Keywords
IDENTIFICATION, DIFFERENTIATION, GSK3-LIKE KINASES, SECRETORY PEPTIDE, TRANSCRIPTION FACTORS, PHOSPHORYLATION SITES, RECEPTOR KINASES, ARABIDOPSIS-THALIANA, ASYMMETRIC CELL-DIVISION, BIOSYNTHESIS

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MLA
Gudesblat, Gustavo, Joanna Schneider-Pizon, Camilla Betti, et al. “SPEECHLESS Integrates Brassinosteroid and Stomata Signalling Pathways.” NATURE CELL BIOLOGY 14.5 (2012): 548–U214. Print.
APA
Gudesblat, G., Schneider-Pizon, J., Betti, C., Mayerhofer, J., Vanhoutte, I., van Dongen, W., Boeren, S., et al. (2012). SPEECHLESS integrates brassinosteroid and stomata signalling pathways. NATURE CELL BIOLOGY, 14(5), 548–U214.
Chicago author-date
Gudesblat, Gustavo, Joanna Schneider-Pizon, Camilla Betti, Juliane Mayerhofer, Isabelle Vanhoutte, Walter van Dongen, Sjef Boeren, et al. 2012. “SPEECHLESS Integrates Brassinosteroid and Stomata Signalling Pathways.” Nature Cell Biology 14 (5): 548–U214.
Chicago author-date (all authors)
Gudesblat, Gustavo, Joanna Schneider-Pizon, Camilla Betti, Juliane Mayerhofer, Isabelle Vanhoutte, Walter van Dongen, Sjef Boeren, Miroslava Zhiponova, Sacco de Vries, Claudia Jonak, and Eugenia Russinova. 2012. “SPEECHLESS Integrates Brassinosteroid and Stomata Signalling Pathways.” Nature Cell Biology 14 (5): 548–U214.
Vancouver
1.
Gudesblat G, Schneider-Pizon J, Betti C, Mayerhofer J, Vanhoutte I, van Dongen W, et al. SPEECHLESS integrates brassinosteroid and stomata signalling pathways. NATURE CELL BIOLOGY. 2012;14(5):548–U214.
IEEE
[1]
G. Gudesblat et al., “SPEECHLESS integrates brassinosteroid and stomata signalling pathways,” NATURE CELL BIOLOGY, vol. 14, no. 5, pp. 548-U214, 2012.
@article{2914778,
  abstract     = {Stomatal formation is regulated by multiple developmental and environmental signals, but how these signals are integrated to control this process is not fully understood(1). In Arabidopsis thaliana, the basic helix-loop-helix transcription factor SPEECHLESS (SPCH) regulates the entry, amplifying and spacing divisions that occur during stomatal lineage development. SPCH activity is negatively regulated by mitogen-activated protein kinase (MAPK)-mediated phosphorylation(2). Here, we show that in addition to MAPKs, SPCH activity is also modulated by brassinosteroid (BR) signalling. The GSK3/SHAGGY-like kinase BIN2 (BR INSENSITIVE2) phosphorylates residues overlapping those targeted by the MAPKs, as well as four residues in the amino-terminal region of the protein outside the MAPK target domain. These phosphorylation events antagonize SPCH activity and limit epidermal cell proliferation. Conversely, inhibition of BIN2 activity in vivo stabilizes SPCH and triggers excessive stomatal and non-stomatal cell formation. We demonstrate that through phosphorylation inputs from both MAPKs and BIN2, SPCH serves as an integration node for stomata and BR signalling pathways to control stomatal development in Arabidopsis.},
  author       = {Gudesblat, Gustavo and Schneider-Pizon, Joanna and Betti, Camilla and Mayerhofer, Juliane and Vanhoutte, Isabelle and van Dongen, Walter and Boeren, Sjef and Zhiponova, Miroslava and de Vries, Sacco and Jonak, Claudia and Russinova, Eugenia},
  issn         = {1465-7392},
  journal      = {NATURE CELL BIOLOGY},
  keywords     = {IDENTIFICATION,DIFFERENTIATION,GSK3-LIKE KINASES,SECRETORY PEPTIDE,TRANSCRIPTION FACTORS,PHOSPHORYLATION SITES,RECEPTOR KINASES,ARABIDOPSIS-THALIANA,ASYMMETRIC CELL-DIVISION,BIOSYNTHESIS},
  language     = {eng},
  number       = {5},
  pages        = {548--U214},
  title        = {SPEECHLESS integrates brassinosteroid and stomata signalling pathways},
  url          = {http://dx.doi.org/10.1038/ncb2471},
  volume       = {14},
  year         = {2012},
}

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