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SPEECHLESS integrates brassinosteroid and stomata signalling pathways

Gustavo Gudesblat UGent, Joanna Schneider-Pizon, Camilla Betti UGent, Juliane Mayerhofer, Isabelle Vanhoutte UGent, Walter van Dongen, Sjef Boeren, Miroslava Zhiponova UGent, Sacco de Vries and Claudia Jonak, et al. (2012) NATURE CELL BIOLOGY. 14(5). p.548-U214
abstract
Stomatal formation is regulated by multiple developmental and environmental signals, but how these signals are integrated to control this process is not fully understood(1). In Arabidopsis thaliana, the basic helix-loop-helix transcription factor SPEECHLESS (SPCH) regulates the entry, amplifying and spacing divisions that occur during stomatal lineage development. SPCH activity is negatively regulated by mitogen-activated protein kinase (MAPK)-mediated phosphorylation(2). Here, we show that in addition to MAPKs, SPCH activity is also modulated by brassinosteroid (BR) signalling. The GSK3/SHAGGY-like kinase BIN2 (BR INSENSITIVE2) phosphorylates residues overlapping those targeted by the MAPKs, as well as four residues in the amino-terminal region of the protein outside the MAPK target domain. These phosphorylation events antagonize SPCH activity and limit epidermal cell proliferation. Conversely, inhibition of BIN2 activity in vivo stabilizes SPCH and triggers excessive stomatal and non-stomatal cell formation. We demonstrate that through phosphorylation inputs from both MAPKs and BIN2, SPCH serves as an integration node for stomata and BR signalling pathways to control stomatal development in Arabidopsis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
IDENTIFICATION, DIFFERENTIATION, GSK3-LIKE KINASES, SECRETORY PEPTIDE, TRANSCRIPTION FACTORS, PHOSPHORYLATION SITES, RECEPTOR KINASES, ARABIDOPSIS-THALIANA, ASYMMETRIC CELL-DIVISION, BIOSYNTHESIS
journal title
NATURE CELL BIOLOGY
Nat. Cell Biol.
volume
14
issue
5
pages
548 - U214
Web of Science type
Article
Web of Science id
000303598300016
JCR category
CELL BIOLOGY
JCR impact factor
20.761 (2012)
JCR rank
6/181 (2012)
JCR quartile
1 (2012)
ISSN
1465-7392
DOI
10.1038/ncb2471
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2914778
handle
http://hdl.handle.net/1854/LU-2914778
date created
2012-06-21 13:56:13
date last changed
2012-06-25 15:49:19
@article{2914778,
  abstract     = {Stomatal formation is regulated by multiple developmental and environmental signals, but how these signals are integrated to control this process is not fully understood(1). In Arabidopsis thaliana, the basic helix-loop-helix transcription factor SPEECHLESS (SPCH) regulates the entry, amplifying and spacing divisions that occur during stomatal lineage development. SPCH activity is negatively regulated by mitogen-activated protein kinase (MAPK)-mediated phosphorylation(2). Here, we show that in addition to MAPKs, SPCH activity is also modulated by brassinosteroid (BR) signalling. The GSK3/SHAGGY-like kinase BIN2 (BR INSENSITIVE2) phosphorylates residues overlapping those targeted by the MAPKs, as well as four residues in the amino-terminal region of the protein outside the MAPK target domain. These phosphorylation events antagonize SPCH activity and limit epidermal cell proliferation. Conversely, inhibition of BIN2 activity in vivo stabilizes SPCH and triggers excessive stomatal and non-stomatal cell formation. We demonstrate that through phosphorylation inputs from both MAPKs and BIN2, SPCH serves as an integration node for stomata and BR signalling pathways to control stomatal development in Arabidopsis.},
  author       = {Gudesblat, Gustavo and Schneider-Pizon, Joanna and Betti, Camilla and Mayerhofer, Juliane and Vanhoutte, Isabelle and van Dongen, Walter and Boeren, Sjef and Zhiponova, Miroslava and de Vries, Sacco and Jonak, Claudia and Russinova, Eugenia},
  issn         = {1465-7392},
  journal      = {NATURE CELL BIOLOGY},
  keyword      = {IDENTIFICATION,DIFFERENTIATION,GSK3-LIKE KINASES,SECRETORY PEPTIDE,TRANSCRIPTION FACTORS,PHOSPHORYLATION SITES,RECEPTOR KINASES,ARABIDOPSIS-THALIANA,ASYMMETRIC CELL-DIVISION,BIOSYNTHESIS},
  language     = {eng},
  number       = {5},
  pages        = {548--U214},
  title        = {SPEECHLESS integrates brassinosteroid and stomata signalling pathways},
  url          = {http://dx.doi.org/10.1038/ncb2471},
  volume       = {14},
  year         = {2012},
}

Chicago
Gudesblat, Gustavo, Joanna Schneider-Pizon, Camilla Betti, Juliane Mayerhofer, Isabelle Vanhoutte, Walter van Dongen, Sjef Boeren, et al. 2012. “SPEECHLESS Integrates Brassinosteroid and Stomata Signalling Pathways.” Nature Cell Biology 14 (5): 548–U214.
APA
Gudesblat, G., Schneider-Pizon, J., Betti, C., Mayerhofer, J., Vanhoutte, I., van Dongen, W., Boeren, S., et al. (2012). SPEECHLESS integrates brassinosteroid and stomata signalling pathways. NATURE CELL BIOLOGY, 14(5), 548–U214.
Vancouver
1.
Gudesblat G, Schneider-Pizon J, Betti C, Mayerhofer J, Vanhoutte I, van Dongen W, et al. SPEECHLESS integrates brassinosteroid and stomata signalling pathways. NATURE CELL BIOLOGY. 2012;14(5):548–U214.
MLA
Gudesblat, Gustavo, Joanna Schneider-Pizon, Camilla Betti, et al. “SPEECHLESS Integrates Brassinosteroid and Stomata Signalling Pathways.” NATURE CELL BIOLOGY 14.5 (2012): 548–U214. Print.