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pFiD188, the linear virulence plasmid of Rhodococcus fascians D188

Isolde Francis UGent, Annick De Keyser UGent, Philippe De Backer UGent, Carmen Simón-Mateo, Jutta Kalkus, Ine Pertry UGent, Wilson Ardiles Diaz UGent, Riet De Rycke UGent, Olivier M Vandeputte and Mondher El Jaziri, et al. (2012) MOLECULAR PLANT-MICROBE INTERACTIONS. 25(5). p.637-647
abstract
Rhodococcus fascians is currently the only phytopathogen of which the virulence genes occur on a linear plasmid. To get insight into the origin of this replicon and into the virulence strategy of this broad-spectrum phytopathogen, the sequence of the linear plasmid of strain D188, pFiD188, was determined. Analysis of the 198,917 bp revealed four syntenic regions with linear plasmids of R. erythropolis, R. jostii, and R. opacus, suggesting a common origin of these replicons. Mutational analysis of pFi_086 and pFi_102, similar to cutinases and type IV peptidases, respectively, showed that conserved region R2 was involved in plasmid dispersal and pointed toward a novel function for actino-bacterial cutinases in conjugation. Additionally, pFiD188 had three regions that were unique for R. fascians. Functional analysis of the stk and nrp loci of regions U2 and U3, respectively, indicated that their role in symptom development was limited compared with that of the previously identified fas, aft, and hyp virulence loci situated in region U1. Thus, pFiD188 is a typical rhodococcal linear plasmid with a composite structure that encodes core functions involved in plasmid maintenance and accessory functions, some possibly acquired through horizontal gene transfer, implicated in virulence and the interaction with the host.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
COMPLETE NUCLEOTIDE-SEQUENCE, BIOTECHNOLOGICAL APPLICATIONS, MYCOBACTERIUM-TUBERCULOSIS, CORYNEBACTERIUM-FASCIANS, PATHOGENICITY ISLANDS, AVIRULENT STRAINS, PLANT DEVELOPMENT, BINDING PROTEIN, STREPTOMYCES, GENOME
journal title
MOLECULAR PLANT-MICROBE INTERACTIONS
Mol. Plant-Microbe Interact.
volume
25
issue
5
pages
637 - 647
Web of Science type
Article
Web of Science id
000303083000004
JCR category
PLANT SCIENCES
JCR impact factor
4.307 (2012)
JCR rank
17/193 (2012)
JCR quartile
1 (2012)
ISSN
0894-0282
DOI
10.1094/MPMI-08-11-0215
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2914680
handle
http://hdl.handle.net/1854/LU-2914680
date created
2012-06-21 13:39:49
date last changed
2012-06-21 17:26:16
@article{2914680,
  abstract     = {Rhodococcus fascians is currently the only phytopathogen of which the virulence genes occur on a linear plasmid. To get insight into the origin of this replicon and into the virulence strategy of this broad-spectrum phytopathogen, the sequence of the linear plasmid of strain D188, pFiD188, was determined. Analysis of the 198,917 bp revealed four syntenic regions with linear plasmids of R. erythropolis, R. jostii, and R. opacus, suggesting a common origin of these replicons. Mutational analysis of pFi\_086 and pFi\_102, similar to cutinases and type IV peptidases, respectively, showed that conserved region R2 was involved in plasmid dispersal and pointed toward a novel function for actino-bacterial cutinases in conjugation. Additionally, pFiD188 had three regions that were unique for R. fascians. Functional analysis of the stk and nrp loci of regions U2 and U3, respectively, indicated that their role in symptom development was limited compared with that of the previously identified fas, aft, and hyp virulence loci situated in region U1. Thus, pFiD188 is a typical rhodococcal linear plasmid with a composite structure that encodes core functions involved in plasmid maintenance and accessory functions, some possibly acquired through horizontal gene transfer, implicated in virulence and the interaction with the host.},
  author       = {Francis, Isolde and De Keyser, Annick and De Backer, Philippe and Sim{\'o}n-Mateo, Carmen and Kalkus, Jutta and Pertry, Ine and Ardiles Diaz, Wilson and De Rycke, Riet and Vandeputte, Olivier M and El Jaziri, Mondher and Holsters, Marcella and Vereecke, Danny},
  issn         = {0894-0282},
  journal      = {MOLECULAR PLANT-MICROBE INTERACTIONS},
  keyword      = {COMPLETE NUCLEOTIDE-SEQUENCE,BIOTECHNOLOGICAL APPLICATIONS,MYCOBACTERIUM-TUBERCULOSIS,CORYNEBACTERIUM-FASCIANS,PATHOGENICITY ISLANDS,AVIRULENT STRAINS,PLANT DEVELOPMENT,BINDING PROTEIN,STREPTOMYCES,GENOME},
  language     = {eng},
  number       = {5},
  pages        = {637--647},
  title        = {pFiD188, the linear virulence plasmid of Rhodococcus fascians D188},
  url          = {http://dx.doi.org/10.1094/MPMI-08-11-0215},
  volume       = {25},
  year         = {2012},
}

Chicago
Francis, Isolde, Annick De Keyser, Philippe De Backer, Carmen Simón-Mateo, Jutta Kalkus, Ine Pertry, Wilson Ardiles Diaz, et al. 2012. “pFiD188, the Linear Virulence Plasmid of Rhodococcus Fascians D188.” Molecular Plant-microbe Interactions 25 (5): 637–647.
APA
Francis, I., De Keyser, A., De Backer, P., Simón-Mateo, C., Kalkus, J., Pertry, I., Ardiles Diaz, W., et al. (2012). pFiD188, the linear virulence plasmid of Rhodococcus fascians D188. MOLECULAR PLANT-MICROBE INTERACTIONS, 25(5), 637–647.
Vancouver
1.
Francis I, De Keyser A, De Backer P, Simón-Mateo C, Kalkus J, Pertry I, et al. pFiD188, the linear virulence plasmid of Rhodococcus fascians D188. MOLECULAR PLANT-MICROBE INTERACTIONS. 2012;25(5):637–47.
MLA
Francis, Isolde, Annick De Keyser, Philippe De Backer, et al. “pFiD188, the Linear Virulence Plasmid of Rhodococcus Fascians D188.” MOLECULAR PLANT-MICROBE INTERACTIONS 25.5 (2012): 637–647. Print.