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pFiD188, the linear virulence plasmid of Rhodococcus fascians D188

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Abstract
Rhodococcus fascians is currently the only phytopathogen of which the virulence genes occur on a linear plasmid. To get insight into the origin of this replicon and into the virulence strategy of this broad-spectrum phytopathogen, the sequence of the linear plasmid of strain D188, pFiD188, was determined. Analysis of the 198,917 bp revealed four syntenic regions with linear plasmids of R. erythropolis, R. jostii, and R. opacus, suggesting a common origin of these replicons. Mutational analysis of pFi_086 and pFi_102, similar to cutinases and type IV peptidases, respectively, showed that conserved region R2 was involved in plasmid dispersal and pointed toward a novel function for actino-bacterial cutinases in conjugation. Additionally, pFiD188 had three regions that were unique for R. fascians. Functional analysis of the stk and nrp loci of regions U2 and U3, respectively, indicated that their role in symptom development was limited compared with that of the previously identified fas, aft, and hyp virulence loci situated in region U1. Thus, pFiD188 is a typical rhodococcal linear plasmid with a composite structure that encodes core functions involved in plasmid maintenance and accessory functions, some possibly acquired through horizontal gene transfer, implicated in virulence and the interaction with the host.
Keywords
COMPLETE NUCLEOTIDE-SEQUENCE, BIOTECHNOLOGICAL APPLICATIONS, MYCOBACTERIUM-TUBERCULOSIS, CORYNEBACTERIUM-FASCIANS, PATHOGENICITY ISLANDS, AVIRULENT STRAINS, PLANT DEVELOPMENT, BINDING PROTEIN, STREPTOMYCES, GENOME

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Chicago
Francis, Isolde, Annick De Keyser, Philippe De Backer, Carmen Simón-Mateo, Jutta Kalkus, Ine Pertry, Wilson Ardiles Diaz, et al. 2012. “pFiD188, the Linear Virulence Plasmid of Rhodococcus Fascians D188.” Molecular Plant-microbe Interactions 25 (5): 637–647.
APA
Francis, Isolde, De Keyser, A., De Backer, P., Simón-Mateo, C., Kalkus, J., Pertry, I., Ardiles Diaz, W., et al. (2012). pFiD188, the linear virulence plasmid of Rhodococcus fascians D188. MOLECULAR PLANT-MICROBE INTERACTIONS, 25(5), 637–647.
Vancouver
1.
Francis I, De Keyser A, De Backer P, Simón-Mateo C, Kalkus J, Pertry I, et al. pFiD188, the linear virulence plasmid of Rhodococcus fascians D188. MOLECULAR PLANT-MICROBE INTERACTIONS. 2012;25(5):637–47.
MLA
Francis, Isolde, Annick De Keyser, Philippe De Backer, et al. “pFiD188, the Linear Virulence Plasmid of Rhodococcus Fascians D188.” MOLECULAR PLANT-MICROBE INTERACTIONS 25.5 (2012): 637–647. Print.
@article{2914680,
  abstract     = {Rhodococcus fascians is currently the only phytopathogen of which the virulence genes occur on a linear plasmid. To get insight into the origin of this replicon and into the virulence strategy of this broad-spectrum phytopathogen, the sequence of the linear plasmid of strain D188, pFiD188, was determined. Analysis of the 198,917 bp revealed four syntenic regions with linear plasmids of R. erythropolis, R. jostii, and R. opacus, suggesting a common origin of these replicons. Mutational analysis of pFi\_086 and pFi\_102, similar to cutinases and type IV peptidases, respectively, showed that conserved region R2 was involved in plasmid dispersal and pointed toward a novel function for actino-bacterial cutinases in conjugation. Additionally, pFiD188 had three regions that were unique for R. fascians. Functional analysis of the stk and nrp loci of regions U2 and U3, respectively, indicated that their role in symptom development was limited compared with that of the previously identified fas, aft, and hyp virulence loci situated in region U1. Thus, pFiD188 is a typical rhodococcal linear plasmid with a composite structure that encodes core functions involved in plasmid maintenance and accessory functions, some possibly acquired through horizontal gene transfer, implicated in virulence and the interaction with the host.},
  author       = {Francis, Isolde and De Keyser, Annick and De Backer, Philippe and Sim{\'o}n-Mateo, Carmen and Kalkus, Jutta and Pertry, Ine and Ardiles Diaz, Wilson and De Rycke, Riet and Vandeputte, Olivier M and El Jaziri, Mondher and Holsters, Marcella and Vereecke, Danny},
  issn         = {0894-0282},
  journal      = {MOLECULAR PLANT-MICROBE INTERACTIONS},
  keyword      = {COMPLETE NUCLEOTIDE-SEQUENCE,BIOTECHNOLOGICAL APPLICATIONS,MYCOBACTERIUM-TUBERCULOSIS,CORYNEBACTERIUM-FASCIANS,PATHOGENICITY ISLANDS,AVIRULENT STRAINS,PLANT DEVELOPMENT,BINDING PROTEIN,STREPTOMYCES,GENOME},
  language     = {eng},
  number       = {5},
  pages        = {637--647},
  title        = {pFiD188, the linear virulence plasmid of Rhodococcus fascians D188},
  url          = {http://dx.doi.org/10.1094/MPMI-08-11-0215},
  volume       = {25},
  year         = {2012},
}

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