Ghent University Academic Bibliography

Advanced

Role of inflammasomes in host defense against Citrobacter rodentium infection

Zhiping Liu, Md Hasan Zaki, Peter Vogel, Prajwal Gurung, B Brett Finlay, Wanyin Deng, Mohamed Lamkanfi UGent and Thirumala-Devi Kanneganti (2012) JOURNAL OF BIOLOGICAL CHEMISTRY. 287(20). p.16955-16964
abstract
Citrobacter rodentium is an enteric bacterial pathogen of the mouse intestinal tract that triggers inflammatory responses resembling those of humans infected with enteropathogenic and enterohemorrhagic Escherichia coli. Inflammasome signaling is emerging as a central regulator of inflammatory and host responses to several pathogens, but the in vivo role of inflammasome signaling in host defense against C. rodentium has not been characterized. Here, we show that mice lacking the inflammasome components Nlrp3, Nlrc4, and caspase-1 were hypersusceptible to C. rodentium-induced gastrointestinal inflammation. This was due to defective interleukin (IL)-1 beta and IL-18 production given that il-1 beta(-/-) and il-18(-/-) mice also suffered from increased bacterial burdens and exacerbated histopathology. C. rodentium specifically activated the Nlrp3 inflammasome in in vitro-infected macrophages independently of a functional bacterial type III secretion system. Thus, production of IL-1 beta and IL-18 downstream of the Nlrp3 and Nlrc4 inflammasomes plays a critical role in host defense against enteric infections caused by C. rodentium.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
VIRULENCE, MYD88, CASPASE-1, SECRETION, ACTIVATION, INTERLEUKIN-1-BETA, DISEASE, EFFACING PATHOGEN, MICE DEFICIENT, INDUCED COLITIS
journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
J. Biol. Chem.
volume
287
issue
20
pages
16955 - 16964
Web of Science type
Article
Web of Science id
000304030900080
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
4.651 (2012)
JCR rank
61/288 (2012)
JCR quartile
1 (2012)
ISSN
0021-9258
DOI
10.1074/jbc.M112.358705
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2914514
handle
http://hdl.handle.net/1854/LU-2914514
date created
2012-06-21 11:58:01
date last changed
2012-06-25 09:53:08
@article{2914514,
  abstract     = {Citrobacter rodentium is an enteric bacterial pathogen of the mouse intestinal tract that triggers inflammatory responses resembling those of humans infected with enteropathogenic and enterohemorrhagic Escherichia coli. Inflammasome signaling is emerging as a central regulator of inflammatory and host responses to several pathogens, but the in vivo role of inflammasome signaling in host defense against C. rodentium has not been characterized. Here, we show that mice lacking the inflammasome components Nlrp3, Nlrc4, and caspase-1 were hypersusceptible to C. rodentium-induced gastrointestinal inflammation. This was due to defective interleukin (IL)-1 beta and IL-18 production given that il-1 beta(-/-) and il-18(-/-) mice also suffered from increased bacterial burdens and exacerbated histopathology. C. rodentium specifically activated the Nlrp3 inflammasome in in vitro-infected macrophages independently of a functional bacterial type III secretion system. Thus, production of IL-1 beta and IL-18 downstream of the Nlrp3 and Nlrc4 inflammasomes plays a critical role in host defense against enteric infections caused by C. rodentium.},
  author       = {Liu, Zhiping and Zaki, Md Hasan and Vogel, Peter and Gurung, Prajwal and Finlay, B Brett and Deng, Wanyin and Lamkanfi, Mohamed and Kanneganti, Thirumala-Devi},
  issn         = {0021-9258},
  journal      = {JOURNAL OF BIOLOGICAL CHEMISTRY},
  keyword      = {VIRULENCE,MYD88,CASPASE-1,SECRETION,ACTIVATION,INTERLEUKIN-1-BETA,DISEASE,EFFACING PATHOGEN,MICE DEFICIENT,INDUCED COLITIS},
  language     = {eng},
  number       = {20},
  pages        = {16955--16964},
  title        = {Role of inflammasomes in host defense against Citrobacter rodentium infection},
  url          = {http://dx.doi.org/10.1074/jbc.M112.358705},
  volume       = {287},
  year         = {2012},
}

Chicago
Liu, Zhiping, Md Hasan Zaki, Peter Vogel, Prajwal Gurung, B Brett Finlay, Wanyin Deng, Mohamed Lamkanfi, and Thirumala-Devi Kanneganti. 2012. “Role of Inflammasomes in Host Defense Against Citrobacter Rodentium Infection.” Journal of Biological Chemistry 287 (20): 16955–16964.
APA
Liu, Zhiping, Zaki, M. H., Vogel, P., Gurung, P., Finlay, B. B., Deng, W., Lamkanfi, M., et al. (2012). Role of inflammasomes in host defense against Citrobacter rodentium infection. JOURNAL OF BIOLOGICAL CHEMISTRY, 287(20), 16955–16964.
Vancouver
1.
Liu Z, Zaki MH, Vogel P, Gurung P, Finlay BB, Deng W, et al. Role of inflammasomes in host defense against Citrobacter rodentium infection. JOURNAL OF BIOLOGICAL CHEMISTRY. 2012;287(20):16955–64.
MLA
Liu, Zhiping, Md Hasan Zaki, Peter Vogel, et al. “Role of Inflammasomes in Host Defense Against Citrobacter Rodentium Infection.” JOURNAL OF BIOLOGICAL CHEMISTRY 287.20 (2012): 16955–16964. Print.