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Role of inflammasomes in host defense against Citrobacter rodentium infection

(2012) JOURNAL OF BIOLOGICAL CHEMISTRY. 287(20). p.16955-16964
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Abstract
Citrobacter rodentium is an enteric bacterial pathogen of the mouse intestinal tract that triggers inflammatory responses resembling those of humans infected with enteropathogenic and enterohemorrhagic Escherichia coli. Inflammasome signaling is emerging as a central regulator of inflammatory and host responses to several pathogens, but the in vivo role of inflammasome signaling in host defense against C. rodentium has not been characterized. Here, we show that mice lacking the inflammasome components Nlrp3, Nlrc4, and caspase-1 were hypersusceptible to C. rodentium-induced gastrointestinal inflammation. This was due to defective interleukin (IL)-1 beta and IL-18 production given that il-1 beta(-/-) and il-18(-/-) mice also suffered from increased bacterial burdens and exacerbated histopathology. C. rodentium specifically activated the Nlrp3 inflammasome in in vitro-infected macrophages independently of a functional bacterial type III secretion system. Thus, production of IL-1 beta and IL-18 downstream of the Nlrp3 and Nlrc4 inflammasomes plays a critical role in host defense against enteric infections caused by C. rodentium.
Keywords
VIRULENCE, MYD88, CASPASE-1, SECRETION, ACTIVATION, INTERLEUKIN-1-BETA, DISEASE, EFFACING PATHOGEN, MICE DEFICIENT, INDUCED COLITIS

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Chicago
Liu, Zhiping, Md Hasan Zaki, Peter Vogel, Prajwal Gurung, B Brett Finlay, Wanyin Deng, Mohamed Lamkanfi, and Thirumala-Devi Kanneganti. 2012. “Role of Inflammasomes in Host Defense Against Citrobacter Rodentium Infection.” Journal of Biological Chemistry 287 (20): 16955–16964.
APA
Liu, Zhiping, Zaki, M. H., Vogel, P., Gurung, P., Finlay, B. B., Deng, W., Lamkanfi, M., et al. (2012). Role of inflammasomes in host defense against Citrobacter rodentium infection. JOURNAL OF BIOLOGICAL CHEMISTRY, 287(20), 16955–16964.
Vancouver
1.
Liu Z, Zaki MH, Vogel P, Gurung P, Finlay BB, Deng W, et al. Role of inflammasomes in host defense against Citrobacter rodentium infection. JOURNAL OF BIOLOGICAL CHEMISTRY. 2012;287(20):16955–64.
MLA
Liu, Zhiping, Md Hasan Zaki, Peter Vogel, et al. “Role of Inflammasomes in Host Defense Against Citrobacter Rodentium Infection.” JOURNAL OF BIOLOGICAL CHEMISTRY 287.20 (2012): 16955–16964. Print.
@article{2914514,
  abstract     = {Citrobacter rodentium is an enteric bacterial pathogen of the mouse intestinal tract that triggers inflammatory responses resembling those of humans infected with enteropathogenic and enterohemorrhagic Escherichia coli. Inflammasome signaling is emerging as a central regulator of inflammatory and host responses to several pathogens, but the in vivo role of inflammasome signaling in host defense against C. rodentium has not been characterized. Here, we show that mice lacking the inflammasome components Nlrp3, Nlrc4, and caspase-1 were hypersusceptible to C. rodentium-induced gastrointestinal inflammation. This was due to defective interleukin (IL)-1 beta and IL-18 production given that il-1 beta(-/-) and il-18(-/-) mice also suffered from increased bacterial burdens and exacerbated histopathology. C. rodentium specifically activated the Nlrp3 inflammasome in in vitro-infected macrophages independently of a functional bacterial type III secretion system. Thus, production of IL-1 beta and IL-18 downstream of the Nlrp3 and Nlrc4 inflammasomes plays a critical role in host defense against enteric infections caused by C. rodentium.},
  author       = {Liu, Zhiping and Zaki, Md Hasan and Vogel, Peter and Gurung, Prajwal and Finlay, B Brett and Deng, Wanyin and Lamkanfi, Mohamed and Kanneganti, Thirumala-Devi},
  issn         = {0021-9258},
  journal      = {JOURNAL OF BIOLOGICAL CHEMISTRY},
  keyword      = {VIRULENCE,MYD88,CASPASE-1,SECRETION,ACTIVATION,INTERLEUKIN-1-BETA,DISEASE,EFFACING PATHOGEN,MICE DEFICIENT,INDUCED COLITIS},
  language     = {eng},
  number       = {20},
  pages        = {16955--16964},
  title        = {Role of inflammasomes in host defense against Citrobacter rodentium infection},
  url          = {http://dx.doi.org/10.1074/jbc.M112.358705},
  volume       = {287},
  year         = {2012},
}

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