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U-HPLC-MS/MS to quantify liposoluble antioxidants in red-ripe tomatoes, grown under different salt stress levels

Lieven Van Meulebroek UGent, Lynn Vanhaecke UGent, Tom De Swaef UGent, Kathy Steppe UGent and Hubert De Brabander UGent (2012) JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 60(2). p.566-573
abstract
The growing interest of consumers in healthy food challenges growers to continuously improve the nutritional quality of their crops. In this research, the potential of a more saline growth environment for improved antioxidant concentration in tomato fruit was studied, and an U-HPLC-MS/MS method for the determination of lycopene, beta-carotene, and alpha-tocopherol was optimized. Analytes were thereby separated on a 1.9 mu m Hypersil GOLD C-18 column and quantified on a TSQ Vantage triple-quadropole mass spectrometer. The method displayed a short analysis time (6 min), a high specificity, and an excellent repeatability (<= 6.39%). Furthermore, it was demonstrated that the electrical conductivity level of the applied nutrient solution did not unambiguously influence antioxidant concentration in tomato fruits. Future research should focus on moderate salt stress only and should aim at reducing natural variation by more closely controlling the growth environment and a more objective determination of the ripening degree.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
antioxidants, tomato, salt stress, ultrahigh-performance liquid chromatography, tandem mass spectrometry, liquid-liquid extraction, electrical conductivity, LIQUID-CHROMATOGRAPHY, MASS-SPECTROMETRY, FRUIT-QUALITY, HUMAN PLASMA, CAROTENOIDS, CONDUCTIVITY, IRRIGATION, COMPONENTS, PRESSURE, LYCOPENE
journal title
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
J. Agric. Food Chem.
volume
60
issue
2
pages
566 - 573
Web of Science type
Article
Web of Science id
000299584600004
JCR category
AGRICULTURE, MULTIDISCIPLINARY
JCR impact factor
2.906 (2012)
JCR rank
1/56 (2012)
JCR quartile
1 (2012)
ISSN
0021-8561
DOI
10.1021/jf2028329
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2914322
handle
http://hdl.handle.net/1854/LU-2914322
date created
2012-06-21 11:06:44
date last changed
2012-06-22 15:40:15
@article{2914322,
  abstract     = {The growing interest of consumers in healthy food challenges growers to continuously improve the nutritional quality of their crops. In this research, the potential of a more saline growth environment for improved antioxidant concentration in tomato fruit was studied, and an U-HPLC-MS/MS method for the determination of lycopene, beta-carotene, and alpha-tocopherol was optimized. Analytes were thereby separated on a 1.9 mu m Hypersil GOLD C-18 column and quantified on a TSQ Vantage triple-quadropole mass spectrometer. The method displayed a short analysis time (6 min), a high specificity, and an excellent repeatability ({\textlangle}= 6.39\%). Furthermore, it was demonstrated that the electrical conductivity level of the applied nutrient solution did not unambiguously influence antioxidant concentration in tomato fruits. Future research should focus on moderate salt stress only and should aim at reducing natural variation by more closely controlling the growth environment and a more objective determination of the ripening degree.},
  author       = {Van Meulebroek, Lieven and Vanhaecke, Lynn and De Swaef, Tom and Steppe, Kathy and De Brabander, Hubert},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keyword      = {antioxidants,tomato,salt stress,ultrahigh-performance liquid chromatography,tandem mass spectrometry,liquid-liquid extraction,electrical conductivity,LIQUID-CHROMATOGRAPHY,MASS-SPECTROMETRY,FRUIT-QUALITY,HUMAN PLASMA,CAROTENOIDS,CONDUCTIVITY,IRRIGATION,COMPONENTS,PRESSURE,LYCOPENE},
  language     = {eng},
  number       = {2},
  pages        = {566--573},
  title        = {U-HPLC-MS/MS to quantify liposoluble antioxidants in red-ripe tomatoes, grown under different salt stress levels},
  url          = {http://dx.doi.org/10.1021/jf2028329},
  volume       = {60},
  year         = {2012},
}

Chicago
Van Meulebroek, Lieven, Lynn Vanhaecke, Tom De Swaef, Kathy Steppe, and Hubert De Brabander. 2012. “U-HPLC-MS/MS to Quantify Liposoluble Antioxidants in Red-ripe Tomatoes, Grown Under Different Salt Stress Levels.” Journal of Agricultural and Food Chemistry 60 (2): 566–573.
APA
Van Meulebroek, L., Vanhaecke, L., De Swaef, T., Steppe, K., & De Brabander, H. (2012). U-HPLC-MS/MS to quantify liposoluble antioxidants in red-ripe tomatoes, grown under different salt stress levels. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 60(2), 566–573.
Vancouver
1.
Van Meulebroek L, Vanhaecke L, De Swaef T, Steppe K, De Brabander H. U-HPLC-MS/MS to quantify liposoluble antioxidants in red-ripe tomatoes, grown under different salt stress levels. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2012;60(2):566–73.
MLA
Van Meulebroek, Lieven, Lynn Vanhaecke, Tom De Swaef, et al. “U-HPLC-MS/MS to Quantify Liposoluble Antioxidants in Red-ripe Tomatoes, Grown Under Different Salt Stress Levels.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 60.2 (2012): 566–573. Print.