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Validation of a method for simultaneous isolation of shiga toxin-producing Escherichia coli O26, O103, O111, and O145 from minced beef by an international ring-trial

Karen Verstraete, Lieven De Zutter UGent, Joris Robyn, Georges Daube, Lieve Herman, Marc Heyndrickx UGent, Marie-Athenais de Schaetzen and Koen De Reu (2012) FOODBORNE PATHOGENS AND DISEASE. 9(5). p.412-417
abstract
An isolation method described by Posse et al. (FEMS Microbiol Lett 2008; 282: 124-131) was satisfactorily validated in an international ring-trial using artificially contaminated minced beef samples. Until now, no validated method existed for the simultaneous isolation of Shiga toxin-producing Escherichia coli serogroups O26, O103, O111, and O145 in food. Twelve laboratories from five European countries participated and received 16 inoculated beef samples contaminated with cold-stressed cells of the four serogroups O26, O103, O111, and O145 in two levels (approximately 30 and 300 CFU 25 g(-1)) in duplicate. In addition, they received four non-inoculated samples. The isolation protocol comprised a selective enrichment step, a selective isolation step on a non-O157 agar plate differentiating the serogroups by color, followed by confirmation by plating on confirmation agar media and agglutination. All laboratories were able to isolate the inoculated serogroups from the samples, both for the high and the low inoculation level. Results did not differ whether in-house-prepared or ready-to-use non-O157 agar plates were used, demonstrating that by following the instructions laboratories managed to perform the complete protocol with success.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PCR, FOOD, SEROTYPES, O157, CATTLE FECES, IMMUNOMAGNETIC SEPARATION
journal title
FOODBORNE PATHOGENS AND DISEASE
Foodborne Pathog. Dis.
volume
9
issue
5
pages
412 - 417
Web of Science type
Article
Web of Science id
000303595800005
JCR category
FOOD SCIENCE & TECHNOLOGY
JCR impact factor
2.283 (2012)
JCR rank
28/123 (2012)
JCR quartile
1 (2012)
ISSN
1535-3141
DOI
10.1089/fpd.2011.0998
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
2914021
handle
http://hdl.handle.net/1854/LU-2914021
date created
2012-06-21 09:58:10
date last changed
2012-07-05 16:48:00
@article{2914021,
  abstract     = {An isolation method described by Posse et al. (FEMS Microbiol Lett 2008; 282: 124-131) was satisfactorily validated in an international ring-trial using artificially contaminated minced beef samples. Until now, no validated method existed for the simultaneous isolation of Shiga toxin-producing Escherichia coli serogroups O26, O103, O111, and O145 in food. Twelve laboratories from five European countries participated and received 16 inoculated beef samples contaminated with cold-stressed cells of the four serogroups O26, O103, O111, and O145 in two levels (approximately 30 and 300 CFU 25 g(-1)) in duplicate. In addition, they received four non-inoculated samples. The isolation protocol comprised a selective enrichment step, a selective isolation step on a non-O157 agar plate differentiating the serogroups by color, followed by confirmation by plating on confirmation agar media and agglutination. All laboratories were able to isolate the inoculated serogroups from the samples, both for the high and the low inoculation level. Results did not differ whether in-house-prepared or ready-to-use non-O157 agar plates were used, demonstrating that by following the instructions laboratories managed to perform the complete protocol with success.},
  author       = {Verstraete, Karen and De Zutter, Lieven and Robyn, Joris and Daube, Georges and Herman, Lieve and Heyndrickx, Marc and de Schaetzen, Marie-Athenais and De Reu, Koen},
  issn         = {1535-3141},
  journal      = {FOODBORNE PATHOGENS AND DISEASE},
  keyword      = {PCR,FOOD,SEROTYPES,O157,CATTLE FECES,IMMUNOMAGNETIC SEPARATION},
  language     = {eng},
  number       = {5},
  pages        = {412--417},
  title        = {Validation of a method for simultaneous isolation of shiga toxin-producing Escherichia coli O26, O103, O111, and O145 from minced beef by an international ring-trial},
  url          = {http://dx.doi.org/10.1089/fpd.2011.0998},
  volume       = {9},
  year         = {2012},
}

Chicago
Verstraete, Karen, Lieven De Zutter, Joris Robyn, Georges Daube, Lieve Herman, Marc Heyndrickx, Marie-Athenais de Schaetzen, and Koen De Reu. 2012. “Validation of a Method for Simultaneous Isolation of Shiga Toxin-producing Escherichia Coli O26, O103, O111, and O145 from Minced Beef by an International Ring-trial.” Foodborne Pathogens and Disease 9 (5): 412–417.
APA
Verstraete, Karen, De Zutter, L., Robyn, J., Daube, G., Herman, L., Heyndrickx, M., de Schaetzen, M.-A., et al. (2012). Validation of a method for simultaneous isolation of shiga toxin-producing Escherichia coli O26, O103, O111, and O145 from minced beef by an international ring-trial. FOODBORNE PATHOGENS AND DISEASE, 9(5), 412–417.
Vancouver
1.
Verstraete K, De Zutter L, Robyn J, Daube G, Herman L, Heyndrickx M, et al. Validation of a method for simultaneous isolation of shiga toxin-producing Escherichia coli O26, O103, O111, and O145 from minced beef by an international ring-trial. FOODBORNE PATHOGENS AND DISEASE. 2012;9(5):412–7.
MLA
Verstraete, Karen, Lieven De Zutter, Joris Robyn, et al. “Validation of a Method for Simultaneous Isolation of Shiga Toxin-producing Escherichia Coli O26, O103, O111, and O145 from Minced Beef by an International Ring-trial.” FOODBORNE PATHOGENS AND DISEASE 9.5 (2012): 412–417. Print.