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Stem/progenitor cells in non-lactating versus lactating equine mammary gland

(2012) STEM CELLS AND DEVELOPMENT. 21(16). p.3055-3067
Author
Organization
Abstract
The mammary gland is a highly regenerative organ that can undergo multiple cycles of proliferation, lactation, and involution. Based on the facts that (i) mammary stem/progenitor cells (MaSC) are proposed to be the driving forces behind mammary growth and function and (ii) variation exists between mammalian species with regard to physiological and pathological functioning of this organ, we believe that studying MaSC from different mammals is of great comparative interest. Over the years, important data has been gathered on MaSC of men and mice, although knowledge on MaSC in other mammals remains limited. Therefore, the aim of this work was to isolate and characterize MaSC from the mammary gland of horses. Hereby, our salient findings were that the isolated equine cells met the 2 in vitro hallmark properties of stem cells, namely the ability to self-renew and to differentiate into multiple cell lineages. Moreover, the cells were immunophenotyped using markers for CD29, CD44, CD49f, and Ki67. Finally, we propose the mammosphere assay as a valuable in vitro assay to study MaSC during different physiological phases since it was observed that equine lactating mammary gland contains significantly more mammosphere-initiating cells than the inactive, nonlactating gland (a reflection of MaSC self-renewal) and, moreover, that these spheres were significantly larger in size upon initial cultivation (a reflection of progenitor cell proliferation). Taken together, this study not only extends the current knowledge of mammary gland biology, but also benefits the comparative approach to study and compare MaSC in different mammalian species.
Keywords
DIFFERENTIATION, TUMORS, CULTURE, EXPRESSION, FUNCTIONAL-CHARACTERIZATION, IN-VITRO, ADULT HUMAN BREAST, MESENCHYMAL STROMAL CELLS, EPIDERMAL-GROWTH-FACTOR, EPITHELIAL STEM-CELLS

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Citation

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MLA
Spaas, Jan et al. “Stem/progenitor Cells in Non-lactating Versus Lactating Equine Mammary Gland.” STEM CELLS AND DEVELOPMENT 21.16 (2012): 3055–3067. Print.
APA
Spaas, J., Chiers, K., Bussche, L., Burvenich, C., & Van de Walle, G. (2012). Stem/progenitor cells in non-lactating versus lactating equine mammary gland. STEM CELLS AND DEVELOPMENT, 21(16), 3055–3067.
Chicago author-date
Spaas, Jan, Koen Chiers, Leen Bussche, Christian Burvenich, and Gerlinde Van de Walle. 2012. “Stem/progenitor Cells in Non-lactating Versus Lactating Equine Mammary Gland.” Stem Cells and Development 21 (16): 3055–3067.
Chicago author-date (all authors)
Spaas, Jan, Koen Chiers, Leen Bussche, Christian Burvenich, and Gerlinde Van de Walle. 2012. “Stem/progenitor Cells in Non-lactating Versus Lactating Equine Mammary Gland.” Stem Cells and Development 21 (16): 3055–3067.
Vancouver
1.
Spaas J, Chiers K, Bussche L, Burvenich C, Van de Walle G. Stem/progenitor cells in non-lactating versus lactating equine mammary gland. STEM CELLS AND DEVELOPMENT. 2012;21(16):3055–67.
IEEE
[1]
J. Spaas, K. Chiers, L. Bussche, C. Burvenich, and G. Van de Walle, “Stem/progenitor cells in non-lactating versus lactating equine mammary gland,” STEM CELLS AND DEVELOPMENT, vol. 21, no. 16, pp. 3055–3067, 2012.
@article{2911417,
  abstract     = {The mammary gland is a highly regenerative organ that can undergo multiple cycles of proliferation, lactation, and involution. Based on the facts that (i) mammary stem/progenitor cells (MaSC) are proposed to be the driving forces behind mammary growth and function and (ii) variation exists between mammalian species with regard to physiological and pathological functioning of this organ, we believe that studying MaSC from different mammals is of great comparative interest. Over the years, important data has been gathered on MaSC of men and mice, although knowledge on MaSC in other mammals remains limited. Therefore, the aim of this work was to isolate and characterize MaSC from the mammary gland of horses. Hereby, our salient findings were that the isolated equine cells met the 2 in vitro hallmark properties of stem cells, namely the ability to self-renew and to differentiate into multiple cell lineages. Moreover, the cells were immunophenotyped using markers for CD29, CD44, CD49f, and Ki67. Finally, we propose the mammosphere assay as a valuable in vitro assay to study MaSC during different physiological phases since it was observed that equine lactating mammary gland contains significantly more mammosphere-initiating cells than the inactive, nonlactating gland (a reflection of MaSC self-renewal) and, moreover, that these spheres were significantly larger in size upon initial cultivation (a reflection of progenitor cell proliferation). Taken together, this study not only extends the current knowledge of mammary gland biology, but also benefits the comparative approach to study and compare MaSC in different mammalian species.},
  author       = {Spaas, Jan and Chiers, Koen and Bussche, Leen and Burvenich, Christian and Van de Walle, Gerlinde},
  issn         = {1547-3287},
  journal      = {STEM CELLS AND DEVELOPMENT},
  keywords     = {DIFFERENTIATION,TUMORS,CULTURE,EXPRESSION,FUNCTIONAL-CHARACTERIZATION,IN-VITRO,ADULT HUMAN BREAST,MESENCHYMAL STROMAL CELLS,EPIDERMAL-GROWTH-FACTOR,EPITHELIAL STEM-CELLS},
  language     = {eng},
  number       = {16},
  pages        = {3055--3067},
  title        = {Stem/progenitor cells in non-lactating versus lactating equine mammary gland},
  url          = {http://dx.doi.org/10.1089/scd.2012.0042},
  volume       = {21},
  year         = {2012},
}

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